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Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients.
Int Arch Allergy Immunol. 2019; 180(2):150-158.IA

Abstract

INTRODUCTION

Regulatory lymphocytes (CD4+ T regulatory cells [Treg], CD8+ Treg, and B regulatory cells [Breg]) play a critical role in immune homeostasis and tolerance. Common variable immunodeficiency (CVID) is associated with increased susceptibility to infections and increased frequency of inflammatory and autoimmune diseases. CD4+ Treg cell abnormalities have been reported in CVID; however, CD8+ Treg cells have not been reported in CVID. The objective of this study was to evaluate CD4+ Treg and CD8+ Treg cells in CVID patients.

METHODS

In 25 patients with CVID and age-matched healthy controls, Treg cells, evaluated in freshly isolated peripheral blood mononuclear cells (natural; nCD4+ Treg and nCD8+ Treg) and following in vitro activation with anti-CD3/CD28 monoclonal antibodies (induced; iCD4+ Treg and iCD8+ Treg) as well as Breg cells were analyzed with specific monoclonal antibodies and isotype controls using flow cytometry.

RESULTS

The proportions of nCD4+ Treg (CD4+ CD127low CD25high FoxP3+), iCD4+ Treg (CD4+ CD127low CD25high FoxP3+), iCD8+ Treg (CD8+ CD25high CD183+ FoxP3+), and Breg (CD19+ CD24high CD38high) lymphocytes were significantly lower in patients with CVID than in controls.

CONCLUSIONS

Altered regulatory lymphocytes may play a role in the pathogenesis and autoimmunity and inflammation associated with CVID.

Authors+Show Affiliations

Division of Basic and Clinical Immunology, University of California, Irvine, California, USA.Division of Basic and Clinical Immunology, University of California, Irvine, California, USA.Division of Basic and Clinical Immunology, University of California, Irvine, California, USA.Division of Basic and Clinical Immunology, University of California, Irvine, California, USA, sgupta@uci.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31284281

Citation

Yesillik, Sait, et al. "Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients." International Archives of Allergy and Immunology, vol. 180, no. 2, 2019, pp. 150-158.
Yesillik S, Agrawal S, Gollapudi SV, et al. Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients. Int Arch Allergy Immunol. 2019;180(2):150-158.
Yesillik, S., Agrawal, S., Gollapudi, S. V., & Gupta, S. (2019). Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients. International Archives of Allergy and Immunology, 180(2), 150-158. https://doi.org/10.1159/000501457
Yesillik S, et al. Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients. Int Arch Allergy Immunol. 2019;180(2):150-158. PubMed PMID: 31284281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic Analysis of CD4+ Treg, CD8+ Treg, and Breg Cells in Adult Common Variable Immunodeficiency Patients. AU - Yesillik,Sait, AU - Agrawal,Sudhanshu, AU - Gollapudi,Sastry V, AU - Gupta,Sudhir, Y1 - 2019/07/08/ PY - 2019/05/14/received PY - 2019/06/11/accepted PY - 2019/7/10/pubmed PY - 2019/11/5/medline PY - 2019/7/9/entrez KW - B regulatory cells KW - CD4 T regulatory cells KW - CD8 T regulatory cells KW - Common variable immunodeficiency SP - 150 EP - 158 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 180 IS - 2 N2 - INTRODUCTION: Regulatory lymphocytes (CD4+ T regulatory cells [Treg], CD8+ Treg, and B regulatory cells [Breg]) play a critical role in immune homeostasis and tolerance. Common variable immunodeficiency (CVID) is associated with increased susceptibility to infections and increased frequency of inflammatory and autoimmune diseases. CD4+ Treg cell abnormalities have been reported in CVID; however, CD8+ Treg cells have not been reported in CVID. The objective of this study was to evaluate CD4+ Treg and CD8+ Treg cells in CVID patients. METHODS: In 25 patients with CVID and age-matched healthy controls, Treg cells, evaluated in freshly isolated peripheral blood mononuclear cells (natural; nCD4+ Treg and nCD8+ Treg) and following in vitro activation with anti-CD3/CD28 monoclonal antibodies (induced; iCD4+ Treg and iCD8+ Treg) as well as Breg cells were analyzed with specific monoclonal antibodies and isotype controls using flow cytometry. RESULTS: The proportions of nCD4+ Treg (CD4+ CD127low CD25high FoxP3+), iCD4+ Treg (CD4+ CD127low CD25high FoxP3+), iCD8+ Treg (CD8+ CD25high CD183+ FoxP3+), and Breg (CD19+ CD24high CD38high) lymphocytes were significantly lower in patients with CVID than in controls. CONCLUSIONS: Altered regulatory lymphocytes may play a role in the pathogenesis and autoimmunity and inflammation associated with CVID. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/31284281/Phenotypic_Analysis_of_CD4+_Treg_CD8+_Treg_and_Breg_Cells_in_Adult_Common_Variable_Immunodeficiency_Patients_ L2 - https://www.karger.com?DOI=10.1159/000501457 DB - PRIME DP - Unbound Medicine ER -