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Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties.
Polymers (Basel) 2019; 11(7)P

Abstract

A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides EPS B3-15 and EPS T14 and the poly-γ-glutamic acid γ-PGA-APA were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa), as well as fungi (Candida albicans) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against E. coli, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for EPS B3-15-Ag NPs and EPS T14-Ag NPs (Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL).

Authors+Show Affiliations

Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. ascala@unime.it.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.Nanobiophotonics Center, Interdisciplinary Research Institute in Bio-Nano-Sciences and Faculty of Physics, Babes-Bolyai University, T Laurian 42, 400271 Cluj-Napoca, Romania.Nanobiophotonics Center, Interdisciplinary Research Institute in Bio-Nano-Sciences and Faculty of Physics, Babes-Bolyai University, T Laurian 42, 400271 Cluj-Napoca, Romania.Nanostructured Materials and Bio-Nano-Interfaces Center, Interdisciplinary Research Institute in BioNano-Sciences, Babes-Bolyai University, T. Laurian 42, 400271 Cluj-Napoca, Romania.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31284651

Citation

Scala, Angela, et al. "Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles With Antimicrobial Properties." Polymers, vol. 11, no. 7, 2019.
Scala A, Piperno A, Hada A, et al. Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties. Polymers (Basel). 2019;11(7).
Scala, A., Piperno, A., Hada, A., Astilean, S., Vulpoi, A., Ginestra, G., ... Gugliandolo, C. (2019). Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties. Polymers, 11(7), doi:10.3390/polym11071157.
Scala A, et al. Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles With Antimicrobial Properties. Polymers (Basel). 2019 Jul 7;11(7) PubMed PMID: 31284651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties. AU - Scala,Angela, AU - Piperno,Anna, AU - Hada,Alexandru, AU - Astilean,Simion, AU - Vulpoi,Adriana, AU - Ginestra,Giovanna, AU - Marino,Andreana, AU - Nostro,Antonia, AU - Zammuto,Vincenzo, AU - Gugliandolo,Concetta, Y1 - 2019/07/07/ PY - 2019/06/11/received PY - 2019/07/03/revised PY - 2019/07/04/accepted PY - 2019/7/10/entrez PY - 2019/7/10/pubmed PY - 2019/7/10/medline KW - Ag nanoparticles KW - AgCl nanoparticles KW - Au nanoparticles KW - C. albicans KW - E. coli KW - EPS KW - P. aeruginosa KW - S. aureus JF - Polymers JO - Polymers (Basel) VL - 11 IS - 7 N2 - A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides EPS B3-15 and EPS T14 and the poly-γ-glutamic acid γ-PGA-APA were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV-Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa), as well as fungi (Candida albicans) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against E. coli, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV-Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for EPS B3-15-Ag NPs and EPS T14-Ag NPs (Minimum Inhibitory Concentration, MIC: 9.37-45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25-75 µg/mL). SN - 2073-4360 UR - https://www.unboundmedicine.com/medline/citation/31284651/Marine_Bacterial_Exopolymers-Mediated_Green_Synthesis_of_Noble_Metal_Nanoparticles_with_Antimicrobial_Properties L2 - http://www.mdpi.com/resolver?pii=polym11071157 DB - PRIME DP - Unbound Medicine ER -