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Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.
Nat Methods. 2019 08; 16(8):707-710.NM

Abstract

Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.

Authors+Show Affiliations

Department of Chemistry, KU Leuven, Heverlee, Belgium.University Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France.Department of Chemistry, KU Leuven, Heverlee, Belgium.Department of Chemistry, KU Leuven, Heverlee, Belgium.University Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France.University Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France.University Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France.Department of Chemistry, KU Leuven, Heverlee, Belgium.Department of Chemistry, KU Leuven, Heverlee, Belgium. peter.dedecker@kuleuven.be.University Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France. dominique.bourgeois@ibs.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31285624

Citation

De Zitter, Elke, et al. "Mechanistic Investigation of mEos4b Reveals a Strategy to Reduce Track Interruptions in SptPALM." Nature Methods, vol. 16, no. 8, 2019, pp. 707-710.
De Zitter E, Thédié D, Mönkemöller V, et al. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. Nat Methods. 2019;16(8):707-710.
De Zitter, E., Thédié, D., Mönkemöller, V., Hugelier, S., Beaudouin, J., Adam, V., Byrdin, M., Van Meervelt, L., Dedecker, P., & Bourgeois, D. (2019). Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. Nature Methods, 16(8), 707-710. https://doi.org/10.1038/s41592-019-0462-3
De Zitter E, et al. Mechanistic Investigation of mEos4b Reveals a Strategy to Reduce Track Interruptions in SptPALM. Nat Methods. 2019;16(8):707-710. PubMed PMID: 31285624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. AU - De Zitter,Elke, AU - Thédié,Daniel, AU - Mönkemöller,Viola, AU - Hugelier,Siewert, AU - Beaudouin,Joël, AU - Adam,Virgile, AU - Byrdin,Martin, AU - Van Meervelt,Luc, AU - Dedecker,Peter, AU - Bourgeois,Dominique, Y1 - 2019/07/08/ PY - 2018/11/20/received PY - 2019/05/24/accepted PY - 2019/7/10/pubmed PY - 2019/11/9/medline PY - 2019/7/10/entrez SP - 707 EP - 710 JF - Nature methods JO - Nat. Methods VL - 16 IS - 8 N2 - Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. SN - 1548-7105 UR - https://www.unboundmedicine.com/medline/citation/31285624/Mechanistic_investigation_of_mEos4b_reveals_a_strategy_to_reduce_track_interruptions_in_sptPALM L2 - http://dx.doi.org/10.1038/s41592-019-0462-3 DB - PRIME DP - Unbound Medicine ER -