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Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans.
BMC Complement Altern Med 2019; 19(1):164BC

Abstract

BACKGROUND

Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context.

METHODS

C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated.

RESULTS

A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction.

CONCLUSIONS

The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies.

Authors+Show Affiliations

Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.College of Public Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian, 116028, China. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany. wink@uni-heidelberg.de.Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. tewin.t@chula.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31286949

Citation

Rangsinth, Panthakarn, et al. "Leaf Extract of Caesalpinia Mimosoides Enhances Oxidative Stress Resistance and Prolongs Lifespan in Caenorhabditis Elegans." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 164.
Rangsinth P, Prasansuklab A, Duangjan C, et al. Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans. BMC Complement Altern Med. 2019;19(1):164.
Rangsinth, P., Prasansuklab, A., Duangjan, C., Gu, X., Meemon, K., Wink, M., & Tencomnao, T. (2019). Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans. BMC Complementary and Alternative Medicine, 19(1), p. 164. doi:10.1186/s12906-019-2578-5.
Rangsinth P, et al. Leaf Extract of Caesalpinia Mimosoides Enhances Oxidative Stress Resistance and Prolongs Lifespan in Caenorhabditis Elegans. BMC Complement Altern Med. 2019 Jul 8;19(1):164. PubMed PMID: 31286949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leaf extract of Caesalpinia mimosoides enhances oxidative stress resistance and prolongs lifespan in Caenorhabditis elegans. AU - Rangsinth,Panthakarn, AU - Prasansuklab,Anchalee, AU - Duangjan,Chatrawee, AU - Gu,Xiaojie, AU - Meemon,Krai, AU - Wink,Michael, AU - Tencomnao,Tewin, Y1 - 2019/07/08/ PY - 2019/04/15/received PY - 2019/06/26/accepted PY - 2019/7/10/entrez PY - 2019/7/10/pubmed PY - 2019/7/10/medline KW - Aging KW - Antioxidant KW - Caenorhabditis elegans KW - Caesalpinia mimosoides KW - DAF-16 KW - Oxidative stress SP - 164 EP - 164 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Caesalpinia mimosoides, a vegetable consumed in Thailand, has been reported to exhibit in vitro antioxidant properties. The in vivo antioxidant and anti-aging activities have not been investigated. The aim of this research was to study the antioxidant activity of C. mimosoides extracts in Caenorhabditis elegans, a widely used model organism in this context. METHODS: C. elegans were treated with C. mimosoides extracts in a various concentrations. To investigate the protective effects of the extract against oxidative stress, wild-type N2 were used to determine survival rate under oxidative stress and intracellular ROS. To study underlying mechanisms, the mutant strains with GFP reporter gene including TJ356, CF1553, EU1 and LD4 were used to study DAF-16, SOD-3, SKN-1 and GST-4 gene, respectively. Lifespan and aging pigment of the worms were also investigated. RESULTS: A leaf extract of C. mimosoides improved resistance to oxidative stress and reduced intracellular ROS accumulation in nematodes. The antioxidant effects were mediated through the DAF-16/FOXO pathway and SOD-3 expression, whereas the expression of SKN-1 and GST-4 were not altered. The extract also prolonged lifespan and decreased aging pigments, while the body length and brood size of the worms were not affected by the extract, indicating low toxicity and excluding dietary restriction. CONCLUSIONS: The results of this study establish the antioxidant activity of C. mimosoides extract in vivo and suggest its potential as a dietary supplement and alternative medicine to defend against oxidative stress and aging, which should be investigated in intervention studies. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31286949/Leaf_extract_of_Caesalpinia_mimosoides_enhances_oxidative_stress_resistance_and_prolongs_lifespan_in_Caenorhabditis_elegans_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2578-5 DB - PRIME DP - Unbound Medicine ER -