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Nanodiamond for Sample Preparation in Proteomics.
Anal Chem 2019; 91(15):9800-9805AC

Abstract

Protein analysis of potential disease markers in blood is complicated by the fact that proteins in plasma show very different abundances. As a result, high-abundance proteins dominate the analysis, which often render the analysis of low-abundance proteins impossible. Depleting high-abundance proteins is one strategy to solve this problem. Here, we present, for the first time, a very simple approach based on selective binding of serum proteins to the surface of nanodiamonds. In our first proof-of-principle experiments, we were able to detect, on average, eight proteins that are present at a concentration of 1 ng/mL (instead of 0.5 ng/mL in the control without sample preparation). Remarkably, we detect proteins down to a concentration of 400 pg/mL after only one simple depletion step. Among the proteins we could analyze are also numerous disease biomarkers, including markers for multiple cancer forms, cardiovascular diseases, or Alzheimer's disease. Remarkably, many of the biomarkers we find also could not be detected with a state-of-the-art ultrahigh-performance liquid chromatography column (which depletes the 64 most-abundant serum proteins).

Authors+Show Affiliations

Groningen University, University Medical Center Groningen , Antonius Deusinglaan 1 , 9713 AW Groningen , The Netherlands.Groningen University, University Medical Center Groningen , Antonius Deusinglaan 1 , 9713 AW Groningen , The Netherlands.Groningen University, University Medical Center Groningen , Antonius Deusinglaan 1 , 9713 AW Groningen , The Netherlands.Groningen University, University Medical Center Groningen , Antonius Deusinglaan 1 , 9713 AW Groningen , The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31290325

Citation

Perona Martinez, Felipe, et al. "Nanodiamond for Sample Preparation in Proteomics." Analytical Chemistry, vol. 91, no. 15, 2019, pp. 9800-9805.
Perona Martinez F, Nagl A, Guluzade S, et al. Nanodiamond for Sample Preparation in Proteomics. Anal Chem. 2019;91(15):9800-9805.
Perona Martinez, F., Nagl, A., Guluzade, S., & Schirhagl, R. (2019). Nanodiamond for Sample Preparation in Proteomics. Analytical Chemistry, 91(15), pp. 9800-9805. doi:10.1021/acs.analchem.9b01459.
Perona Martinez F, et al. Nanodiamond for Sample Preparation in Proteomics. Anal Chem. 2019 Aug 6;91(15):9800-9805. PubMed PMID: 31290325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanodiamond for Sample Preparation in Proteomics. AU - Perona Martinez,Felipe, AU - Nagl,Andreas, AU - Guluzade,Sona, AU - Schirhagl,Romana, Y1 - 2019/07/23/ PY - 2019/7/11/pubmed PY - 2019/7/11/medline PY - 2019/7/11/entrez SP - 9800 EP - 9805 JF - Analytical chemistry JO - Anal. Chem. VL - 91 IS - 15 N2 - Protein analysis of potential disease markers in blood is complicated by the fact that proteins in plasma show very different abundances. As a result, high-abundance proteins dominate the analysis, which often render the analysis of low-abundance proteins impossible. Depleting high-abundance proteins is one strategy to solve this problem. Here, we present, for the first time, a very simple approach based on selective binding of serum proteins to the surface of nanodiamonds. In our first proof-of-principle experiments, we were able to detect, on average, eight proteins that are present at a concentration of 1 ng/mL (instead of 0.5 ng/mL in the control without sample preparation). Remarkably, we detect proteins down to a concentration of 400 pg/mL after only one simple depletion step. Among the proteins we could analyze are also numerous disease biomarkers, including markers for multiple cancer forms, cardiovascular diseases, or Alzheimer's disease. Remarkably, many of the biomarkers we find also could not be detected with a state-of-the-art ultrahigh-performance liquid chromatography column (which depletes the 64 most-abundant serum proteins). SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/31290325/Nanodiamond_for_Sample_Preparation_in_Proteomics L2 - https://dx.doi.org/10.1021/acs.analchem.9b01459 DB - PRIME DP - Unbound Medicine ER -