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Association of P2RX7 functional variants with localized aggressive periodontitis.
J Periodontal Res. 2020 Jan; 55(1):32-40.JP

Abstract

OBJECTIVE

The purpose of this study was to investigate involvement of the P2X7 receptor in the rare condition, localized aggressive periodontitis.

MATERIAL AND METHODS

Peripheral blood from 220 African Americans (103 with localized aggressive periodontitis and 117 healthy unrelated controls) was stimulated with lipopolysaccharide from E coli and Porphyromonas gingivalis. P2RX7 single nucleotide polymorphisms rs208294 (H155Y), rs1718119 (T348A), rs2230911 (T357S) and rs3751143 (E496A) were genotyped in 103 localized aggressive periodontitis patients and 117 healthy unrelated subjects. We examined genetic association between four P2RX7 single nucleotide polymorphisms and localized aggressive periodontitis, and tested for correlations between the single nucleotide polymorphisms and inflammatory response to lipopolysaccharide in blood samples from these patients.

RESULTS

A significant association with localized aggressive periodontitis was observed with rs1718119 A (Thr) allele (P = 0.0063, odds ratio = 1.904) and with a haplotype containing this allele (P = 0.0075). Additionally, significant correlations with these data were found: the rs1718119 G allele correlated with greater production of IL-6, IL-2 and GM-CSF; the C (His) allele of rs208294 correlated with lower levels of IL-12p40; and the C (Thr) allele of rs2230911 correlated with greater levels of G-CSF.

CONCLUSION

The data from these analyses support a possible biological relationship between P2RX7 genetic variants and inflammatory response in localized aggressive periodontitis patients.

Authors+Show Affiliations

Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, USA. Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA.Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, USA. University of Florida Genetics Institute, Gainesville, Florida, USA.Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, USA.Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, USA.Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, USA.Department of Pharmacotherapy and Translational Research and Center for Pharmacogenomics, University of Florida College of Pharmacy, Gainesville, Florida, USA.Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, USA.Division of Restorative Dentistry & Periodontology, Dublin Dental University Hospital, Trinity College, Dublin, Ireland.Department of Periodontology, University of Florida College of Dentistry, Gainesville, Florida, USA.Division of Periodontology, Department of Oral Health Practice, University of Kentucky College of Dentistry, Lexington, Kentucky, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31292966

Citation

Harris, Theodore H., et al. "Association of P2RX7 Functional Variants With Localized Aggressive Periodontitis." Journal of Periodontal Research, vol. 55, no. 1, 2020, pp. 32-40.
Harris TH, Wallace MR, Huang H, et al. Association of P2RX7 functional variants with localized aggressive periodontitis. J Periodont Res. 2020;55(1):32-40.
Harris, T. H., Wallace, M. R., Huang, H., Li, H., Mohiuddeen, A., Gong, Y., Kompotiati, T., Harrison, P., Aukhil, I., & Shaddox, L. M. (2020). Association of P2RX7 functional variants with localized aggressive periodontitis. Journal of Periodontal Research, 55(1), 32-40. https://doi.org/10.1111/jre.12682
Harris TH, et al. Association of P2RX7 Functional Variants With Localized Aggressive Periodontitis. J Periodont Res. 2020;55(1):32-40. PubMed PMID: 31292966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of P2RX7 functional variants with localized aggressive periodontitis. AU - Harris,Theodore H, AU - Wallace,Margaret R, AU - Huang,Hong, AU - Li,Hua, AU - Mohiuddeen,Azeem, AU - Gong,Yan, AU - Kompotiati,Theodora, AU - Harrison,Peter, AU - Aukhil,Ikramuddin, AU - Shaddox,Luciana M, Y1 - 2019/07/10/ PY - 2019/01/10/received PY - 2019/05/22/revised PY - 2019/06/09/accepted PY - 2019/7/12/pubmed PY - 2020/3/5/medline PY - 2019/7/12/entrez SP - 32 EP - 40 JF - Journal of periodontal research JO - J. Periodont. Res. VL - 55 IS - 1 N2 - OBJECTIVE: The purpose of this study was to investigate involvement of the P2X7 receptor in the rare condition, localized aggressive periodontitis. MATERIAL AND METHODS: Peripheral blood from 220 African Americans (103 with localized aggressive periodontitis and 117 healthy unrelated controls) was stimulated with lipopolysaccharide from E coli and Porphyromonas gingivalis. P2RX7 single nucleotide polymorphisms rs208294 (H155Y), rs1718119 (T348A), rs2230911 (T357S) and rs3751143 (E496A) were genotyped in 103 localized aggressive periodontitis patients and 117 healthy unrelated subjects. We examined genetic association between four P2RX7 single nucleotide polymorphisms and localized aggressive periodontitis, and tested for correlations between the single nucleotide polymorphisms and inflammatory response to lipopolysaccharide in blood samples from these patients. RESULTS: A significant association with localized aggressive periodontitis was observed with rs1718119 A (Thr) allele (P = 0.0063, odds ratio = 1.904) and with a haplotype containing this allele (P = 0.0075). Additionally, significant correlations with these data were found: the rs1718119 G allele correlated with greater production of IL-6, IL-2 and GM-CSF; the C (His) allele of rs208294 correlated with lower levels of IL-12p40; and the C (Thr) allele of rs2230911 correlated with greater levels of G-CSF. CONCLUSION: The data from these analyses support a possible biological relationship between P2RX7 genetic variants and inflammatory response in localized aggressive periodontitis patients. SN - 1600-0765 UR - https://www.unboundmedicine.com/medline/citation/31292966/Association_of_P2RX7_functional_variants_with_localized_aggressive_periodontitis_ L2 - https://doi.org/10.1111/jre.12682 DB - PRIME DP - Unbound Medicine ER -