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[Evaluation of a rapid assay for detection of PBP2a Staphylococcus aureus].
Rev Esp Quimioter 2019; 32(4):370-374RE

Abstract

OBJECTIVE

Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen causing both healthcare-associated and community-acquired infection. Rapid and accurate detection of this pathogen is crucial for the use of appropriate antimicrobial therapy and the control of nosocomial spread.

METHODS

A total of 107 S. aureus strains were assayed for methicillin resistance: Vitek2® (bioMérieux), CHROMagarTM MRSA II (BD Becton Dickinson), disk diffusion in agar for cefoxitin 30 μg and immunochromatography PBP2a SA Culture Colony Test (AlereTM). The results of conventional tests were compared with the "gold standard" PCR test for mecA gene.

RESULTS

Sensitivity and specificity were: disk diffusion for cefoxitin 100% and 100% respectively, Vitek2® 100 and 100%, CHROMagarTM MRSA II 100 and 96%, and ICPBP2a detection 98,25% and 100%.

CONCLUSIONS

ICPBP2a Culture Colony Test (AlereTM) is fast, efficient and economical technique for detection of penicillin binding protein 2a (PBP2a) from isolates. This assay is a useful tool for the management of hospital outbreaks.

Authors+Show Affiliations

Begoña Palop-Borrás, Servicio de Microbiología, Hospital Regional Universitario de Málaga, Avda. Carlos Haya, s/n 29010 Málaga, Spain. bpalop@hotmail.com.

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

31293115

Citation

Sáinz-Rodríguez, R, et al. "[Evaluation of a Rapid Assay for Detection of PBP2a Staphylococcus Aureus]." Revista Espanola De Quimioterapia : Publicacion Oficial De La Sociedad Espanola De Quimioterapia, vol. 32, no. 4, 2019, pp. 370-374.
Sáinz-Rodríguez R, de Toro-Peinado I, Valverde-Troya M, et al. [Evaluation of a rapid assay for detection of PBP2a Staphylococcus aureus]. Rev Esp Quimioter. 2019;32(4):370-374.
Sáinz-Rodríguez, R., de Toro-Peinado, I., Valverde-Troya, M., Bermúdez Ruíz, M. P., & Palop-Borrás, B. (2019). [Evaluation of a rapid assay for detection of PBP2a Staphylococcus aureus]. Revista Espanola De Quimioterapia : Publicacion Oficial De La Sociedad Espanola De Quimioterapia, 32(4), pp. 370-374.
Sáinz-Rodríguez R, et al. [Evaluation of a Rapid Assay for Detection of PBP2a Staphylococcus Aureus]. Rev Esp Quimioter. 2019;32(4):370-374. PubMed PMID: 31293115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Evaluation of a rapid assay for detection of PBP2a Staphylococcus aureus]. AU - Sáinz-Rodríguez,R, AU - de Toro-Peinado,I, AU - Valverde-Troya,M, AU - Bermúdez Ruíz,M P, AU - Palop-Borrás,B, Y1 - 2019/07/10/ PY - 2019/7/12/pubmed PY - 2019/7/12/medline PY - 2019/7/12/entrez SP - 370 EP - 374 JF - Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia JO - Rev Esp Quimioter VL - 32 IS - 4 N2 - OBJECTIVE: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen causing both healthcare-associated and community-acquired infection. Rapid and accurate detection of this pathogen is crucial for the use of appropriate antimicrobial therapy and the control of nosocomial spread. METHODS: A total of 107 S. aureus strains were assayed for methicillin resistance: Vitek2® (bioMérieux), CHROMagarTM MRSA II (BD Becton Dickinson), disk diffusion in agar for cefoxitin 30 μg and immunochromatography PBP2a SA Culture Colony Test (AlereTM). The results of conventional tests were compared with the "gold standard" PCR test for mecA gene. RESULTS: Sensitivity and specificity were: disk diffusion for cefoxitin 100% and 100% respectively, Vitek2® 100 and 100%, CHROMagarTM MRSA II 100 and 96%, and ICPBP2a detection 98,25% and 100%. CONCLUSIONS: ICPBP2a Culture Colony Test (AlereTM) is fast, efficient and economical technique for detection of penicillin binding protein 2a (PBP2a) from isolates. This assay is a useful tool for the management of hospital outbreaks. SN - 1988-9518 UR - https://www.unboundmedicine.com/medline/citation/31293115/[Evaluation_of_a_rapid_assay_for_detection_of_PBP2a_Staphylococcus_aureus] L2 - http://seq.es/abstract/july-10-3-2 DB - PRIME DP - Unbound Medicine ER -