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Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum.
Front Microbiol 2019; 10:1393FM

Abstract

Oenococcus oeni can survive well in wine (an acid-stress environment) and dominate malolactic fermentation (MLF). To demonstrate a possible role of argininosuccinate synthase gene (argG) in the acid tolerance response of O. oeni, a related argG gene was inserted into a plasmid pMG36e and heterologously expressed in Lactobacillus plantarum SL09, a wine isolate belonging to a species of relevant importance in MLF. The expression levels of the argG gene in L. plantarum were analyzed by RT-qPCR, argininosuccinate synthase (ASS) activity and cell properties (amino acids, pH, H+-ATPase activity, and ATP levels) were determined at pH 3.7 in comparison with that at pH 6.3. Results showed that the recombinant strain L. plantarum SL09 (pMG36eargG) exhibited stronger growth performance compared with the control strain (without argG gene), and the expression levels of hsp1, cfa, atp, the citrate and malate metabolic genes were apparently increased under acid stress. In addition, the recombinant strain exhibited 11.0-, 2.0-, 1.9-fold higher ASS activity, H+-ATPase activity and intracellular ATP level, compared with the corresponding values for control strain during acid-stresses condition, which may take responsible for the acid tolerance enhancement of the recombinant strain. This is the first work report on heterologous expression of argG gene, and the results presented in this study will be beneficial for the research on acid stress response of O. oeni.

Authors+Show Affiliations

College of Enology, Northwest A&F University, Yangling, China.Shandong Engineering and Technology Research Center for Ecological Fragile Belt of Yellow River Delta, Binzhou, China.College of Enology, Northwest A&F University, Yangling, China.College of Enology, Northwest A&F University, Yangling, China.College of Enology, Northwest A&F University, Yangling, China. Heyang Experimental and Demonstrational Stations for Grape, Weinan, China. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, China.College of Enology, Northwest A&F University, Yangling, China. Heyang Experimental and Demonstrational Stations for Grape, Weinan, China. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31293541

Citation

Zhao, Hongyu, et al. "Heterologous Expression of Argininosuccinate Synthase From Oenococcus Oeni Enhances the Acid Resistance of Lactobacillus Plantarum." Frontiers in Microbiology, vol. 10, 2019, p. 1393.
Zhao H, Liu L, Peng S, et al. Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum. Front Microbiol. 2019;10:1393.
Zhao, H., Liu, L., Peng, S., Yuan, L., Li, H., & Wang, H. (2019). Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum. Frontiers in Microbiology, 10, p. 1393. doi:10.3389/fmicb.2019.01393.
Zhao H, et al. Heterologous Expression of Argininosuccinate Synthase From Oenococcus Oeni Enhances the Acid Resistance of Lactobacillus Plantarum. Front Microbiol. 2019;10:1393. PubMed PMID: 31293541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum. AU - Zhao,Hongyu, AU - Liu,Longxiang, AU - Peng,Shuai, AU - Yuan,Lin, AU - Li,Hua, AU - Wang,Hua, Y1 - 2019/06/21/ PY - 2019/04/24/received PY - 2019/06/04/accepted PY - 2019/7/12/entrez PY - 2019/7/12/pubmed PY - 2019/7/12/medline KW - Lactobacillus plantarum KW - Oenococcus oeni KW - acid stress KW - argininosuccinate synthase KW - heterologous expression SP - 1393 EP - 1393 JF - Frontiers in microbiology JO - Front Microbiol VL - 10 N2 - Oenococcus oeni can survive well in wine (an acid-stress environment) and dominate malolactic fermentation (MLF). To demonstrate a possible role of argininosuccinate synthase gene (argG) in the acid tolerance response of O. oeni, a related argG gene was inserted into a plasmid pMG36e and heterologously expressed in Lactobacillus plantarum SL09, a wine isolate belonging to a species of relevant importance in MLF. The expression levels of the argG gene in L. plantarum were analyzed by RT-qPCR, argininosuccinate synthase (ASS) activity and cell properties (amino acids, pH, H+-ATPase activity, and ATP levels) were determined at pH 3.7 in comparison with that at pH 6.3. Results showed that the recombinant strain L. plantarum SL09 (pMG36eargG) exhibited stronger growth performance compared with the control strain (without argG gene), and the expression levels of hsp1, cfa, atp, the citrate and malate metabolic genes were apparently increased under acid stress. In addition, the recombinant strain exhibited 11.0-, 2.0-, 1.9-fold higher ASS activity, H+-ATPase activity and intracellular ATP level, compared with the corresponding values for control strain during acid-stresses condition, which may take responsible for the acid tolerance enhancement of the recombinant strain. This is the first work report on heterologous expression of argG gene, and the results presented in this study will be beneficial for the research on acid stress response of O. oeni. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/31293541/Heterologous_Expression_of_Argininosuccinate_Synthase_From_Oenococcus_oeni_Enhances_the_Acid_Resistance_of_Lactobacillus_plantarum L2 - https://dx.doi.org/10.3389/fmicb.2019.01393 DB - PRIME DP - Unbound Medicine ER -