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Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity.
Front Microbiol 2019; 10:1421FM

Abstract

Parapoxvirus of red deer in New Zealand (PVNZ) is a species of the Parapoxvirus genus that causes pustular dermatitis. We identified a cluster of genes in PVNZ that encode three unique chemokine-binding proteins (CBPs) namely ORF112.0, ORF112.3 and ORF112.6. Chemokines are a large family of molecules that direct cell trafficking to sites of inflammation and through lymphatic organs. The PVNZ-CBPs were analyzed by surface plasmon resonance against a broad spectrum of CXC, CC, XC and CX3C chemokines and were found to differ in their specificity and binding affinity. ORF112.0 interacted with chemokines from the CXC, CC and XC classes of chemokines with nM affinities. The ORF112.3 showed a preference for CXC chemokines, while ORF112.6 showed pM affinity binding for CC chemokines. Structural modeling analysis showed alterations in the chemokine binding sites of the CBPs, although the core structure containing two β-sheets and three α-helices being conserved with the other parapoxvirus CBPs. Chemotaxis assays using neutrophils and monocytes revealed inhibitory impact of the CBPs on cell migration. Our results suggest that the PVNZ-CBPs are likely to have evolved through a process of gene duplication and divergence, and may have a role in suppressing inflammation and the anti-viral immune response.

Authors+Show Affiliations

Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.Virus Research Unit, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31293551

Citation

Sharif, Saeed, et al. "Chemokine-Binding Proteins Encoded By Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity." Frontiers in Microbiology, vol. 10, 2019, p. 1421.
Sharif S, Ueda N, Nakatani Y, et al. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity. Front Microbiol. 2019;10:1421.
Sharif, S., Ueda, N., Nakatani, Y., Wise, L. M., Clifton, S., Lateef, Z., ... Fleming, S. B. (2019). Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity. Frontiers in Microbiology, 10, p. 1421. doi:10.3389/fmicb.2019.01421.
Sharif S, et al. Chemokine-Binding Proteins Encoded By Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity. Front Microbiol. 2019;10:1421. PubMed PMID: 31293551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity. AU - Sharif,Saeed, AU - Ueda,Norihito, AU - Nakatani,Yoshio, AU - Wise,Lyn M, AU - Clifton,Sheree, AU - Lateef,Zabeen, AU - Mercer,Andrew A, AU - Fleming,Stephen B, Y1 - 2019/06/25/ PY - 2019/03/13/received PY - 2019/06/05/accepted PY - 2019/7/12/entrez PY - 2019/7/12/pubmed PY - 2019/7/12/medline KW - cell migration KW - chemokine KW - chemokine-binding protein KW - parapoxvirus KW - parapoxvirus of red deer in New Zealand SP - 1421 EP - 1421 JF - Frontiers in microbiology JO - Front Microbiol VL - 10 N2 - Parapoxvirus of red deer in New Zealand (PVNZ) is a species of the Parapoxvirus genus that causes pustular dermatitis. We identified a cluster of genes in PVNZ that encode three unique chemokine-binding proteins (CBPs) namely ORF112.0, ORF112.3 and ORF112.6. Chemokines are a large family of molecules that direct cell trafficking to sites of inflammation and through lymphatic organs. The PVNZ-CBPs were analyzed by surface plasmon resonance against a broad spectrum of CXC, CC, XC and CX3C chemokines and were found to differ in their specificity and binding affinity. ORF112.0 interacted with chemokines from the CXC, CC and XC classes of chemokines with nM affinities. The ORF112.3 showed a preference for CXC chemokines, while ORF112.6 showed pM affinity binding for CC chemokines. Structural modeling analysis showed alterations in the chemokine binding sites of the CBPs, although the core structure containing two β-sheets and three α-helices being conserved with the other parapoxvirus CBPs. Chemotaxis assays using neutrophils and monocytes revealed inhibitory impact of the CBPs on cell migration. Our results suggest that the PVNZ-CBPs are likely to have evolved through a process of gene duplication and divergence, and may have a role in suppressing inflammation and the anti-viral immune response. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/31293551/Chemokine-Binding_Proteins_Encoded_by_Parapoxvirus_of_Red_Deer_of_New_Zealand_Display_Evidence_of_Gene_Duplication_and_Divergence_of_Ligand_Specificity L2 - https://doi.org/10.3389/fmicb.2019.01421 DB - PRIME DP - Unbound Medicine ER -