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The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice.
Front Plant Sci 2019; 10:752FP

Abstract

Mitogen-activated protein kinase (MAPK) cascades play central roles in response to biotic and abiotic stresses. However, the mechanisms by which various MAPK members regulate the plant immune response in rice remain elusive. In this article, to characterize the mechanisms, the knock-out and overexpression mutants of OsMPK15 were constructed and the disease resistance was investigated under the various fungal and bacterial inoculations. The knock-out mutant of OsMPK15 resulted in the constitutive expression of pathogenesis-related (PR) genes, increased accumulation of reactive oxygen species (ROS) triggered by the pathogen-associated molecular pattern (PAMP) elicitor chitin, and significantly enhanced the disease resistance to different races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo), which cause the rice blast and bacterial blight diseases, respectively. On contrary, the expression of PR genes and ROS were down-regulated in the OsMPK15-overexpressing (OsMPK15-OE) lines. Meanwhile, phytohormones such as salicylic acid (SA) and jasmonic acid (JA) were accumulated in the mpk15 mutant lines but decreased in the OsMPK15-OE lines. The expression of SA- and JA-pathway associated genes were significantly upregulated in the mpk15 mutant, whereas it was down regulated in the OsMPK15-OE lines. We conclude that OsMPK15 may negatively regulate the disease resistance through modulating SA- and JA-mediated signaling pathway.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China. Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31293603

Citation

Hong, Yongbo, et al. "The OsMPK15 Negatively Regulates Magnaporthe Oryza and Xoo Disease Resistance Via SA and JA Signaling Pathway in Rice." Frontiers in Plant Science, vol. 10, 2019, p. 752.
Hong Y, Liu Q, Cao Y, et al. The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice. Front Plant Sci. 2019;10:752.
Hong, Y., Liu, Q., Cao, Y., Zhang, Y., Chen, D., Lou, X., ... Cao, L. (2019). The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice. Frontiers in Plant Science, 10, p. 752. doi:10.3389/fpls.2019.00752.
Hong Y, et al. The OsMPK15 Negatively Regulates Magnaporthe Oryza and Xoo Disease Resistance Via SA and JA Signaling Pathway in Rice. Front Plant Sci. 2019;10:752. PubMed PMID: 31293603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice. AU - Hong,Yongbo, AU - Liu,Qunen, AU - Cao,Yongrun, AU - Zhang,Yue, AU - Chen,Daibo, AU - Lou,Xiangyang, AU - Cheng,Shihua, AU - Cao,Liyong, Y1 - 2019/06/21/ PY - 2019/01/24/received PY - 2019/05/23/accepted PY - 2019/7/12/entrez PY - 2019/7/12/pubmed PY - 2019/7/12/medline KW - M. oryzae KW - OsMPK15 KW - PRs KW - ROS KW - SA/JA KW - Xoo KW - rice SP - 752 EP - 752 JF - Frontiers in plant science JO - Front Plant Sci VL - 10 N2 - Mitogen-activated protein kinase (MAPK) cascades play central roles in response to biotic and abiotic stresses. However, the mechanisms by which various MAPK members regulate the plant immune response in rice remain elusive. In this article, to characterize the mechanisms, the knock-out and overexpression mutants of OsMPK15 were constructed and the disease resistance was investigated under the various fungal and bacterial inoculations. The knock-out mutant of OsMPK15 resulted in the constitutive expression of pathogenesis-related (PR) genes, increased accumulation of reactive oxygen species (ROS) triggered by the pathogen-associated molecular pattern (PAMP) elicitor chitin, and significantly enhanced the disease resistance to different races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo), which cause the rice blast and bacterial blight diseases, respectively. On contrary, the expression of PR genes and ROS were down-regulated in the OsMPK15-overexpressing (OsMPK15-OE) lines. Meanwhile, phytohormones such as salicylic acid (SA) and jasmonic acid (JA) were accumulated in the mpk15 mutant lines but decreased in the OsMPK15-OE lines. The expression of SA- and JA-pathway associated genes were significantly upregulated in the mpk15 mutant, whereas it was down regulated in the OsMPK15-OE lines. We conclude that OsMPK15 may negatively regulate the disease resistance through modulating SA- and JA-mediated signaling pathway. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/31293603/The_OsMPK15_Negatively_Regulates_Magnaporthe_oryza_and_Xoo_Disease_Resistance_via_SA_and_JA_Signaling_Pathway_in_Rice L2 - https://dx.doi.org/10.3389/fpls.2019.00752 DB - PRIME DP - Unbound Medicine ER -