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Treponema pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells.

Abstract

The pathological features of syphilis, a disease caused by Treponema pallidum (T. pallidum), are characterized by vascular involvement with endarteritis and periarteritis. Little is known about the interactions of infiltrating immunocytes with human dermal vascular smooth muscle cells (HDVSMCs) in arterioles during the immunopathogenesis of syphilis. In the present study, we demonstrated that stimulation of HDVSMCs with T. pallidum resulted in the upregulated gene transcription and protein expression of interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) in a dose- and time-dependent manner. Moreover, the migration and adhesion of THP-1 cells to HDVSMCs were significantly suppressed by anti-MCP-1 and anti-ICAM-1 neutralizing antibodies, respectively. Further studies revealed that T. pallidum activated the NF-κB signaling pathway in HDVSMCs. Inhibition of NF-κB suppressed T. pallidum-induced IL-6, MCP-1, and ICAM-1 expression. In addition, the migration and adhesion of THP-1 cells to T. pallidum-treated HDVSMCs were significantly decreased by pretreatment with an NF-κB inhibitor. These findings demonstrate that T. pallidum induces the production of IL-6, MCP-1, and ICAM-1 in HDVSMCs and promotes the adherence and migration of THP-1 cells to HDVSMCs through the NF-κB signaling pathway, which may provide new insight into the pathogenesis of T. pallidum infection.

Authors+Show Affiliations

Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China. Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31293985

Citation

Gao, Zheng-Xiang, et al. "Treponema Pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells." Frontiers in Cellular and Infection Microbiology, vol. 9, 2019, p. 220.
Gao ZX, Liu LL, Lin LR, et al. Treponema pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells. Front Cell Infect Microbiol. 2019;9:220.
Gao, Z. X., Liu, L. L., Lin, L. R., Tong, M. L., Liu, F., & Yang, T. C. (2019). Treponema pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells. Frontiers in Cellular and Infection Microbiology, 9, p. 220. doi:10.3389/fcimb.2019.00220.
Gao ZX, et al. Treponema Pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells. Front Cell Infect Microbiol. 2019;9:220. PubMed PMID: 31293985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treponema pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to Promote the Migration and Adhesion of THP-1 Cells. AU - Gao,Zheng-Xiang, AU - Liu,Li-Li, AU - Lin,Li-Rong, AU - Tong,Man-Li, AU - Liu,Fan, AU - Yang,Tian-Ci, Y1 - 2019/06/21/ PY - 2019/03/12/received PY - 2019/06/07/accepted PY - 2019/7/12/entrez PY - 2019/7/12/pubmed PY - 2019/7/12/medline KW - Treponema pallidum KW - adherence KW - cytokine KW - human dermal vascular smooth muscle cells KW - migration SP - 220 EP - 220 JF - Frontiers in cellular and infection microbiology JO - Front Cell Infect Microbiol VL - 9 N2 - The pathological features of syphilis, a disease caused by Treponema pallidum (T. pallidum), are characterized by vascular involvement with endarteritis and periarteritis. Little is known about the interactions of infiltrating immunocytes with human dermal vascular smooth muscle cells (HDVSMCs) in arterioles during the immunopathogenesis of syphilis. In the present study, we demonstrated that stimulation of HDVSMCs with T. pallidum resulted in the upregulated gene transcription and protein expression of interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) in a dose- and time-dependent manner. Moreover, the migration and adhesion of THP-1 cells to HDVSMCs were significantly suppressed by anti-MCP-1 and anti-ICAM-1 neutralizing antibodies, respectively. Further studies revealed that T. pallidum activated the NF-κB signaling pathway in HDVSMCs. Inhibition of NF-κB suppressed T. pallidum-induced IL-6, MCP-1, and ICAM-1 expression. In addition, the migration and adhesion of THP-1 cells to T. pallidum-treated HDVSMCs were significantly decreased by pretreatment with an NF-κB inhibitor. These findings demonstrate that T. pallidum induces the production of IL-6, MCP-1, and ICAM-1 in HDVSMCs and promotes the adherence and migration of THP-1 cells to HDVSMCs through the NF-κB signaling pathway, which may provide new insight into the pathogenesis of T. pallidum infection. SN - 2235-2988 UR - https://www.unboundmedicine.com/medline/citation/31293985/Treponema_pallidum_Induces_the_Secretion_of_HDVSMC_Inflammatory_Cytokines_to_Promote_the_Migration_and_Adhesion_of_THP-1_Cells L2 - https://doi.org/10.3389/fcimb.2019.00220 DB - PRIME DP - Unbound Medicine ER -