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Molecular studies of a rare DR2/LD-5a/DQw3 HLA class II haplotype. Multiple genetic mechanisms in the generation of polymorphic HLA class II genes.
J Immunol. 1988 May 15; 140(10):3631-9.JI

Abstract

A cDNA library was constructed from a homozygous B lymphoblastoid cell line (REM) obtained from an individual of a long isolated American Indian tribe, the Warao. The REM cell line expresses serologically defined determinants, DR2 and DQw3, and the T lymphocyte-defined (Dw/LD) specificity, LD-5a. T cells can recognize differences between FJO (a DR2/DQw1 cell line that expresses the Dw specificity MN2) and REM for both DR and DQ molecules. cNDA clones encoding the polymorphic DR beta 1-, DR beta 2-, DQ beta-, and DQ alpha-chains were sequenced and compared with other DR and DQ gene sequences. The DR beta 1-sequence of REM is identical to the DR beta 1-sequence of FJO; the DR beta 2-sequence is also identical to that of FJO except for one amino acid difference at position 67 in the polymorphic first domain (Leu in REM, Phe in FJO) due to a single point mutation. The DQ beta-sequence is identical to that of DR4/DQw3 haplotype; the DQ alpha is different from the DQ alpha of DR4/DQw3 haplotype and identical to the DQ alpha of both the DR3/DQw2 haplotype of a Raji cell line and the DR5/DQw3 haplotype in deduced amino acid sequence. Taken together, these findings suggest that: 1) a single amino acid difference (position 67) in the third hypervariable region of the first domain of the DR beta 2-chain in the DR2 haplotype is apparently sufficient for stimulating T cell responses; 2) the DQw3 serologic specificity may be defined mainly by the DQ beta-rather than DQ alpha-chain; and 3) multiple genetic events have probably occurred to generate the rarely found REM (DR2/LD-5a/DQw3) haplotype.

Authors+Show Affiliations

Department of Laboratory Medicine/Pathology, University of Minnesota, Minneapolis 55455.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3129499

Citation

Liu, C P., et al. "Molecular Studies of a Rare DR2/LD-5a/DQw3 HLA Class II Haplotype. Multiple Genetic Mechanisms in the Generation of Polymorphic HLA Class II Genes." Journal of Immunology (Baltimore, Md. : 1950), vol. 140, no. 10, 1988, pp. 3631-9.
Liu CP, Bach FH, Wu SK. Molecular studies of a rare DR2/LD-5a/DQw3 HLA class II haplotype. Multiple genetic mechanisms in the generation of polymorphic HLA class II genes. J Immunol. 1988;140(10):3631-9.
Liu, C. P., Bach, F. H., & Wu, S. K. (1988). Molecular studies of a rare DR2/LD-5a/DQw3 HLA class II haplotype. Multiple genetic mechanisms in the generation of polymorphic HLA class II genes. Journal of Immunology (Baltimore, Md. : 1950), 140(10), 3631-9.
Liu CP, Bach FH, Wu SK. Molecular Studies of a Rare DR2/LD-5a/DQw3 HLA Class II Haplotype. Multiple Genetic Mechanisms in the Generation of Polymorphic HLA Class II Genes. J Immunol. 1988 May 15;140(10):3631-9. PubMed PMID: 3129499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular studies of a rare DR2/LD-5a/DQw3 HLA class II haplotype. Multiple genetic mechanisms in the generation of polymorphic HLA class II genes. AU - Liu,C P, AU - Bach,F H, AU - Wu,S K, PY - 1988/5/15/pubmed PY - 1988/5/15/medline PY - 1988/5/15/entrez SP - 3631 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 140 IS - 10 N2 - A cDNA library was constructed from a homozygous B lymphoblastoid cell line (REM) obtained from an individual of a long isolated American Indian tribe, the Warao. The REM cell line expresses serologically defined determinants, DR2 and DQw3, and the T lymphocyte-defined (Dw/LD) specificity, LD-5a. T cells can recognize differences between FJO (a DR2/DQw1 cell line that expresses the Dw specificity MN2) and REM for both DR and DQ molecules. cNDA clones encoding the polymorphic DR beta 1-, DR beta 2-, DQ beta-, and DQ alpha-chains were sequenced and compared with other DR and DQ gene sequences. The DR beta 1-sequence of REM is identical to the DR beta 1-sequence of FJO; the DR beta 2-sequence is also identical to that of FJO except for one amino acid difference at position 67 in the polymorphic first domain (Leu in REM, Phe in FJO) due to a single point mutation. The DQ beta-sequence is identical to that of DR4/DQw3 haplotype; the DQ alpha is different from the DQ alpha of DR4/DQw3 haplotype and identical to the DQ alpha of both the DR3/DQw2 haplotype of a Raji cell line and the DR5/DQw3 haplotype in deduced amino acid sequence. Taken together, these findings suggest that: 1) a single amino acid difference (position 67) in the third hypervariable region of the first domain of the DR beta 2-chain in the DR2 haplotype is apparently sufficient for stimulating T cell responses; 2) the DQw3 serologic specificity may be defined mainly by the DQ beta-rather than DQ alpha-chain; and 3) multiple genetic events have probably occurred to generate the rarely found REM (DR2/LD-5a/DQw3) haplotype. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3129499/Molecular_studies_of_a_rare_DR2/LD_5a/DQw3_HLA_class_II_haplotype__Multiple_genetic_mechanisms_in_the_generation_of_polymorphic_HLA_class_II_genes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3129499 DB - PRIME DP - Unbound Medicine ER -