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The clinical significance and biological function of lncRNA SOCAR in serous ovarian carcinoma.
Gene 2019; 713:143969GENE

Abstract

BACKGROUND

Ovarian cancer (OvCa) is one of the most lethal gynecologic malignancies worldwide. Pelvic and abdominal metastasis is a leading cause for the poor prognosis of OvCa patients. The relationship between long non-coding RNAs (lncRNAs) and OvCa remains unclear. Identifying key lncRNAs related with OvCa metastasis is crucial for research on the mechanism of OvCa metastasis. This study was designed to investigate the role of a novel lncRNA, which we named SOCAR, in serous OvCa.

METHODS

LncRNA microarray and Real-time PCR were used to examine SOCAR expression in high grade serous ovarian cancer (HGSOC) and normal ovary tissues. The proliferation, migration and invasion of OvCa cell lines SKOV-3 and OVCAR-3 were analyzed by CCK-8, Transwell and Scratch wound healing assays. Western blotting was used to detect the expression of Wnt/β-catenin pathway-related proteins.

RESULTS

A novel serous OvCa-related lncRNA, SOCAR, was identified via microarray. SOCAR was overexpressed in primary HGSOC tumors compared with normal ovary tissues, and the expression of SOCAR correlated with progression in HGSOC patients. SOCAR also had higher expression in metastatic HGSOC tissues compared with primary cancer tissues. Moreover, upregulation of SOCAR promoted proliferation, migration and invasion in OvCa cells. Expression of Wnt1, β-catenin and MMP-9 were all increased by SOCAR overexpression.

CONCLUSION

SOCAR is related with HGSOC oncogenesis and progression. It may promote proliferation, migration and invasion in OvCa cells partially by upregulating MMP-9 through the Wnt/β-catenin pathway.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China.Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China.Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China; Department of Obstetrics, Xiamen Maternity and Child Health Care Hospital, Xiamen 361003, China.Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China.Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China.Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China.Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China.Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China.Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City 110001, China.Department of Obstetrics and Gynecology, Shengjing hospital of China Medical University, 36 San Hao Street, Heping District, Shenyang City 110004, China. Electronic address: drzhouxin@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31299360

Citation

Guo, Qixuan, et al. "The Clinical Significance and Biological Function of lncRNA SOCAR in Serous Ovarian Carcinoma." Gene, vol. 713, 2019, p. 143969.
Guo Q, Wang L, Zhu L, et al. The clinical significance and biological function of lncRNA SOCAR in serous ovarian carcinoma. Gene. 2019;713:143969.
Guo, Q., Wang, L., Zhu, L., Lu, X., Song, Y., Sun, J., ... Zhou, X. (2019). The clinical significance and biological function of lncRNA SOCAR in serous ovarian carcinoma. Gene, 713, p. 143969. doi:10.1016/j.gene.2019.143969.
Guo Q, et al. The Clinical Significance and Biological Function of lncRNA SOCAR in Serous Ovarian Carcinoma. Gene. 2019 Sep 10;713:143969. PubMed PMID: 31299360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The clinical significance and biological function of lncRNA SOCAR in serous ovarian carcinoma. AU - Guo,Qixuan, AU - Wang,Ling, AU - Zhu,Linfei, AU - Lu,Xinxin, AU - Song,Yongxi, AU - Sun,Jingxu, AU - Wu,Zhonghua, AU - Shi,Jinxin, AU - Wang,Zhenning, AU - Zhou,Xin, Y1 - 2019/07/09/ PY - 2019/03/21/received PY - 2019/07/04/revised PY - 2019/07/08/accepted PY - 2019/7/13/pubmed PY - 2019/8/10/medline PY - 2019/7/13/entrez KW - Invasion KW - Long non-coding RNA KW - Migration KW - Serous ovarian cancer KW - Wnt/β-catenin pathway SP - 143969 EP - 143969 JF - Gene JO - Gene VL - 713 N2 - BACKGROUND: Ovarian cancer (OvCa) is one of the most lethal gynecologic malignancies worldwide. Pelvic and abdominal metastasis is a leading cause for the poor prognosis of OvCa patients. The relationship between long non-coding RNAs (lncRNAs) and OvCa remains unclear. Identifying key lncRNAs related with OvCa metastasis is crucial for research on the mechanism of OvCa metastasis. This study was designed to investigate the role of a novel lncRNA, which we named SOCAR, in serous OvCa. METHODS: LncRNA microarray and Real-time PCR were used to examine SOCAR expression in high grade serous ovarian cancer (HGSOC) and normal ovary tissues. The proliferation, migration and invasion of OvCa cell lines SKOV-3 and OVCAR-3 were analyzed by CCK-8, Transwell and Scratch wound healing assays. Western blotting was used to detect the expression of Wnt/β-catenin pathway-related proteins. RESULTS: A novel serous OvCa-related lncRNA, SOCAR, was identified via microarray. SOCAR was overexpressed in primary HGSOC tumors compared with normal ovary tissues, and the expression of SOCAR correlated with progression in HGSOC patients. SOCAR also had higher expression in metastatic HGSOC tissues compared with primary cancer tissues. Moreover, upregulation of SOCAR promoted proliferation, migration and invasion in OvCa cells. Expression of Wnt1, β-catenin and MMP-9 were all increased by SOCAR overexpression. CONCLUSION: SOCAR is related with HGSOC oncogenesis and progression. It may promote proliferation, migration and invasion in OvCa cells partially by upregulating MMP-9 through the Wnt/β-catenin pathway. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/31299360/The_clinical_significance_and_biological_function_of_lncRNA_SOCAR_in_serous_ovarian_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(19)30619-5 DB - PRIME DP - Unbound Medicine ER -