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Orf virus circulation in cattle in Turkey.

Abstract

Orf virus (ORFV) causes contagious skin disease that mainly affects sheep and goats with zoonotic potential. However, there is not enough information about the association between ORFV and occurrence of skin disease in cattle. The present study describes outbreaks of ORFV infection in cattle in different provinces that are located in the Aegean, Central Anatolian and Mediterranean regions of Turkey. During the months of June and August 2017, vesicular fluid and scab samples were collected from cattle which had proliferative skin lesions. First, presence of lumpy skin disease virus (LSDV) and bovine herpesvirus 2 (BoHV-2, known as the causative agent of pseudo-lumpy skin disease) were investigated by real time PCR and PCR, respectively. Then, samples tested for the presence of parapoxviruses by PCR using primers specific to major envelope protein gene (B2L). Parapoxvirus DNA was detected in investigated samples whereas LSDV and BoHV-2 DNA were not detected. The analysis of the B2L gene sequences revealed that cattle were infected with ORFV. The isolates in the present study shared 100% sequence identity at the nucleotide and amino acid level when compared with previously characterised Turkish field ORFV isolates from goats in 2016. Results of the study show unusual infection of cattle with ORFV, and suggest that ORFV jumps the host species barrier from goats to cattle.

Authors+Show Affiliations

Department of Virology, Veterinary Faculty, Hatay Mustafa Kemal University, Antakya, 31040 Hatay, Turkey. Electronic address: dr_muratank@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31300096

Citation

Şevik, Murat. "Orf Virus Circulation in Cattle in Turkey." Comparative Immunology, Microbiology and Infectious Diseases, vol. 65, 2019, pp. 1-6.
Şevik M. Orf virus circulation in cattle in Turkey. Comp Immunol Microbiol Infect Dis. 2019;65:1-6.
Şevik, M. (2019). Orf virus circulation in cattle in Turkey. Comparative Immunology, Microbiology and Infectious Diseases, 65, pp. 1-6. doi:10.1016/j.cimid.2019.03.013.
Şevik M. Orf Virus Circulation in Cattle in Turkey. Comp Immunol Microbiol Infect Dis. 2019;65:1-6. PubMed PMID: 31300096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Orf virus circulation in cattle in Turkey. A1 - Şevik,Murat, Y1 - 2019/03/26/ PY - 2019/02/18/received PY - 2019/03/20/accepted PY - 2019/7/14/entrez KW - B2L gene KW - Cattle KW - Orf virus KW - Parapoxvirus KW - Skin disease KW - Turkey SP - 1 EP - 6 JF - Comparative immunology, microbiology and infectious diseases JO - Comp. Immunol. Microbiol. Infect. Dis. VL - 65 N2 - Orf virus (ORFV) causes contagious skin disease that mainly affects sheep and goats with zoonotic potential. However, there is not enough information about the association between ORFV and occurrence of skin disease in cattle. The present study describes outbreaks of ORFV infection in cattle in different provinces that are located in the Aegean, Central Anatolian and Mediterranean regions of Turkey. During the months of June and August 2017, vesicular fluid and scab samples were collected from cattle which had proliferative skin lesions. First, presence of lumpy skin disease virus (LSDV) and bovine herpesvirus 2 (BoHV-2, known as the causative agent of pseudo-lumpy skin disease) were investigated by real time PCR and PCR, respectively. Then, samples tested for the presence of parapoxviruses by PCR using primers specific to major envelope protein gene (B2L). Parapoxvirus DNA was detected in investigated samples whereas LSDV and BoHV-2 DNA were not detected. The analysis of the B2L gene sequences revealed that cattle were infected with ORFV. The isolates in the present study shared 100% sequence identity at the nucleotide and amino acid level when compared with previously characterised Turkish field ORFV isolates from goats in 2016. Results of the study show unusual infection of cattle with ORFV, and suggest that ORFV jumps the host species barrier from goats to cattle. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/31300096/Orf_virus_circulation_in_cattle_in_Turkey L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(19)30067-0 DB - PRIME DP - Unbound Medicine ER -