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Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice.
Biomed Pharmacother. 2019 Oct; 118:109206.BP

Abstract

To investigate effects of berberine exerts on A20 expression and regulation of intestinal epithelial tight junctions via the TNF-α-NF-κB-MLCK pathway in Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D). C57BL/6 wild type (WT) and A20 IEC-KO mice (48 each) were randomly divided into normal control (NC), model control (MC), rifaximin and berberine groups (12 mice per group). An experimental model of IBS-D was established using 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. rifaximin and berberine mice were treated with rifaximin and berberine, respectively. Intestinal epithelial space of WT berberine mice improved more than A20 IEC-KO berberine mice compared to MC mice. WT berberine mice exhibited greater expression of A20 compared with MC mice(P < 0.01). TNF-α, NF-kB p65, MLCK, MLC, TRAF6 and RIP1 levels in A20 IEC-KO and WT berberine mice were all decreased compared to MC mice(P all<0.05). NF-κB p65, MLCK and TRAF6 levels were increased in A20 IEC-KO berberine mice as compared to WT berberine mice (P all<0.05). Intestinal epithelial levels of occludin, claudin-1, ZO-1 and F-actin increased in all berberine mice (P all<0.01-0.05), while occludin, claudin-1, and ZO-1 levels were lower in A20 IEC-KO berberine mice(P < 0.05). Berberine downregulates abnormal activation of the TNF-α-NF-κB-MLCK pathway by upregulating expression of A20 in a mouse model of IBS-D, thereby protecting intestinal epithelial tight junctions and repairing the damage IBS-D causes to the intestinal epithelial barrier.

Authors+Show Affiliations

Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China; School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. Electronic address: houqiuke@gzucm.edu.cn.Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: 1350967609@qq.com.Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: 2751710748@qq.com.Department of Orthopedics, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: huangyongquan@gzucm.edu.cn.Inernational Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: 3022577391@qq.com.Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: 3280881263@qq.com.Department of Preventive Medicine and Health Statistics, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. Electronic address: 175075547@qq.com.Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: 3342168019@qq.com.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. Electronic address: simonhan74@126.com.Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: liufb163@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31306972

Citation

Hou, Qiuke, et al. "Berberine Improves Intestinal Epithelial Tight Junctions By Upregulating A20 Expression in IBS-D Mice." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 118, 2019, p. 109206.
Hou Q, Zhu S, Zhang C, et al. Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice. Biomed Pharmacother. 2019;118:109206.
Hou, Q., Zhu, S., Zhang, C., Huang, Y., Guo, Y., Li, P., Chen, X., Wen, Y., Han, Q., & Liu, F. (2019). Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 118, 109206. https://doi.org/10.1016/j.biopha.2019.109206
Hou Q, et al. Berberine Improves Intestinal Epithelial Tight Junctions By Upregulating A20 Expression in IBS-D Mice. Biomed Pharmacother. 2019;118:109206. PubMed PMID: 31306972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice. AU - Hou,Qiuke, AU - Zhu,Shuilian, AU - Zhang,Changrong, AU - Huang,Yongquan, AU - Guo,Yajuan, AU - Li,Peiwu, AU - Chen,Xinlin, AU - Wen,Yi, AU - Han,Quanbin, AU - Liu,Fengbin, Y1 - 2019/07/12/ PY - 2019/06/01/received PY - 2019/06/25/revised PY - 2019/07/02/accepted PY - 2019/7/16/pubmed PY - 2020/2/13/medline PY - 2019/7/16/entrez KW - A20 KW - Berberine KW - IBS-D KW - Intestinal epithelial barrier KW - TNF-α-NF-κB-MLCK pathway SP - 109206 EP - 109206 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 118 N2 - To investigate effects of berberine exerts on A20 expression and regulation of intestinal epithelial tight junctions via the TNF-α-NF-κB-MLCK pathway in Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D). C57BL/6 wild type (WT) and A20 IEC-KO mice (48 each) were randomly divided into normal control (NC), model control (MC), rifaximin and berberine groups (12 mice per group). An experimental model of IBS-D was established using 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. rifaximin and berberine mice were treated with rifaximin and berberine, respectively. Intestinal epithelial space of WT berberine mice improved more than A20 IEC-KO berberine mice compared to MC mice. WT berberine mice exhibited greater expression of A20 compared with MC mice(P < 0.01). TNF-α, NF-kB p65, MLCK, MLC, TRAF6 and RIP1 levels in A20 IEC-KO and WT berberine mice were all decreased compared to MC mice(P all<0.05). NF-κB p65, MLCK and TRAF6 levels were increased in A20 IEC-KO berberine mice as compared to WT berberine mice (P all<0.05). Intestinal epithelial levels of occludin, claudin-1, ZO-1 and F-actin increased in all berberine mice (P all<0.01-0.05), while occludin, claudin-1, and ZO-1 levels were lower in A20 IEC-KO berberine mice(P < 0.05). Berberine downregulates abnormal activation of the TNF-α-NF-κB-MLCK pathway by upregulating expression of A20 in a mouse model of IBS-D, thereby protecting intestinal epithelial tight junctions and repairing the damage IBS-D causes to the intestinal epithelial barrier. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/31306972/Berberine_improves_intestinal_epithelial_tight_junctions_by_upregulating_A20_expression_in_IBS_D_mice_ DB - PRIME DP - Unbound Medicine ER -