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High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains.
Enzyme Microb Technol 2019; 129:109359EM

Abstract

Thermotolerant yeast Kluyveromyces marxianus can assimilate xylose but cannot produce ethanol from xylose under anaerobic conditions. Here, we constructed two recombinant K. marxianus strains, DMB5 and DMB13, that express xylose reductase (XR), NAD+- or protein-engineered NADP+-dependent xylitol dehydrogenase (XDH), and xylulokinase (XK) from K. marxianus. These strains, together with previously reported strain DMB3-7, which expresses Scheffersomyces stipitis XR and NAD+-dependent XDH and Saccharomyces cerevisiae XK, were compared to evaluate enzymatic activities and ethanol productivities at 30 °C and 40 °C. Unlike the activities of xylose metabolic enzymes in DMB3-7, enzymatic activities of XR, XDH, and XK in both DMB5 and DMB13 hardly decreased even at 40 °C, suggesting that these enzymes from K. marxianus are highly thermostable. The most efficient glucose/xylose co-fermentation at 40 °C was found in DMB13; namely, DMB13 rapidly converted xylose to ethanol, especially after glucose depletion, and showed the highest ethanol yield (0.402 g/g). These findings support the view that alteration of coenzyme specificity of XDH expressed in K. marxianus will be efficacious for high-temperature ethanol production from mixed sugars containing xylose.

Authors+Show Affiliations

Research Institute for Sustainable Chemistry (ISC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan.Research Institute for Sustainable Chemistry (ISC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan; Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8530, Japan.Research Institute for Sustainable Chemistry (ISC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan; Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8530, Japan. Electronic address: a-matsushika@aist.go.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31307575

Citation

Suzuki, Toshihiro, et al. "High-temperature Ethanol Production By a Series of Recombinant Xylose-fermenting Kluyveromyces Marxianus Strains." Enzyme and Microbial Technology, vol. 129, 2019, p. 109359.
Suzuki T, Hoshino T, Matsushika A. High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains. Enzyme Microb Technol. 2019;129:109359.
Suzuki, T., Hoshino, T., & Matsushika, A. (2019). High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains. Enzyme and Microbial Technology, 129, p. 109359. doi:10.1016/j.enzmictec.2019.109359.
Suzuki T, Hoshino T, Matsushika A. High-temperature Ethanol Production By a Series of Recombinant Xylose-fermenting Kluyveromyces Marxianus Strains. Enzyme Microb Technol. 2019;129:109359. PubMed PMID: 31307575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains. AU - Suzuki,Toshihiro, AU - Hoshino,Tamotsu, AU - Matsushika,Akinori, Y1 - 2019/06/14/ PY - 2019/04/15/received PY - 2019/06/11/revised PY - 2019/06/13/accepted PY - 2019/7/17/entrez KW - Coenzyme specificity KW - Ethanol production KW - Kluyveromyces marxianus KW - Xylitol dehydrogenase KW - Xylose fermentation SP - 109359 EP - 109359 JF - Enzyme and microbial technology JO - Enzyme Microb. Technol. VL - 129 N2 - Thermotolerant yeast Kluyveromyces marxianus can assimilate xylose but cannot produce ethanol from xylose under anaerobic conditions. Here, we constructed two recombinant K. marxianus strains, DMB5 and DMB13, that express xylose reductase (XR), NAD+- or protein-engineered NADP+-dependent xylitol dehydrogenase (XDH), and xylulokinase (XK) from K. marxianus. These strains, together with previously reported strain DMB3-7, which expresses Scheffersomyces stipitis XR and NAD+-dependent XDH and Saccharomyces cerevisiae XK, were compared to evaluate enzymatic activities and ethanol productivities at 30 °C and 40 °C. Unlike the activities of xylose metabolic enzymes in DMB3-7, enzymatic activities of XR, XDH, and XK in both DMB5 and DMB13 hardly decreased even at 40 °C, suggesting that these enzymes from K. marxianus are highly thermostable. The most efficient glucose/xylose co-fermentation at 40 °C was found in DMB13; namely, DMB13 rapidly converted xylose to ethanol, especially after glucose depletion, and showed the highest ethanol yield (0.402 g/g). These findings support the view that alteration of coenzyme specificity of XDH expressed in K. marxianus will be efficacious for high-temperature ethanol production from mixed sugars containing xylose. SN - 1879-0909 UR - https://www.unboundmedicine.com/medline/citation/31307575/High-temperature_ethanol_production_by_a_series_of_recombinant_xylose-fermenting_Kluyveromyces_marxianus_strains L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-0229(19)30096-1 DB - PRIME DP - Unbound Medicine ER -