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Hydrocortisone loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles for topical ophthalmic administration: Preparation, characterization and evaluation of ophthalmic toxicity.
Int J Pharm 2019; 568:118519IJ

Abstract

Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles (PHBV-NPs) to encapsulate hydrocortisone (HC) for topical ophthalmic administration were prepared and characterized. The technique used to prepare the nanoparticles (NPs) was emulsification/solvent evaporation. The obtained size was 237.3 ± 2.7 nm, suitable for topical ocular administration. The obtained results for the entrapment efficiency were between 1 and 2.5% and for the drug loading were around 0.5%. The release behaviour of HC from the PHBV-NPs was also analyzed, adjusting this to a Higuchi kinetic model. For the new drug delivery system developed the ocular toxicity profile was determined by viability studies carried out on bovine keratocytes, by a Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) and by a Bovine Corneal Opacity and Permeability assay (BCOP). The obtained results concluded that the new system is no cytotoxic on bovine keratocytes and is neither irritating nor produces any alteration in the transparency and in the permeability of the cornea. Confocal studies were also performed and confirmed that PHBV-NPs are able to penetrate efficiently into the corneal tissue. This novel PHBV-based drug delivery system could be a good option for topical ophthalmic administration of drugs.

Authors+Show Affiliations

Universidade da Coruña, Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Universitaria Politécnica, Serantes, Avda. 19 de Febrero s/n, 15471 Ferrol, Spain; Cellular and Molecular Cardiology Research Unit, Institute of Biomedical Research (IDIS-SERGAS), University Clinical Hospital, Santiago de Compostela, Spain.Universidade da Coruña, Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Universitaria Politécnica, Serantes, Avda. 19 de Febrero s/n, 15471 Ferrol, Spain.Universidade da Coruña, Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Universitaria Politécnica, Serantes, Avda. 19 de Febrero s/n, 15471 Ferrol, Spain. Electronic address: rebeca.bouza@udc.es.Universidade da Coruña, Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Universitaria Politécnica, Serantes, Avda. 19 de Febrero s/n, 15471 Ferrol, Spain.Pharmacy and Pharmaceutical Technology Department and Industrial Pharmacy Institute, Faculty of Pharmacy, University of Santiago de Compostela (USC), Campus Vida, Santiago de Compostela 15782, Spain.Cellular and Molecular Cardiology Research Unit, Institute of Biomedical Research (IDIS-SERGAS), University Clinical Hospital, Santiago de Compostela, Spain; Center for Biomedical Research Network in Cardiovascular Diseases (CIBERCV), Madrid, Spain.Cellular and Molecular Cardiology Research Unit, Institute of Biomedical Research (IDIS-SERGAS), University Clinical Hospital, Santiago de Compostela, Spain; Center for Biomedical Research Network in Cardiovascular Diseases (CIBERCV), Madrid, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31310791

Citation

Álvarez-Álvarez, Lucía, et al. "Hydrocortisone Loaded Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanoparticles for Topical Ophthalmic Administration: Preparation, Characterization and Evaluation of Ophthalmic Toxicity." International Journal of Pharmaceutics, vol. 568, 2019, p. 118519.
Álvarez-Álvarez L, Barral L, Bouza R, et al. Hydrocortisone loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles for topical ophthalmic administration: Preparation, characterization and evaluation of ophthalmic toxicity. Int J Pharm. 2019;568:118519.
Álvarez-Álvarez, L., Barral, L., Bouza, R., Farrag, Y., Otero-Espinar, F., Feijóo-Bandín, S., & Lago, F. (2019). Hydrocortisone loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles for topical ophthalmic administration: Preparation, characterization and evaluation of ophthalmic toxicity. International Journal of Pharmaceutics, 568, p. 118519. doi:10.1016/j.ijpharm.2019.118519.
Álvarez-Álvarez L, et al. Hydrocortisone Loaded Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanoparticles for Topical Ophthalmic Administration: Preparation, Characterization and Evaluation of Ophthalmic Toxicity. Int J Pharm. 2019 Sep 10;568:118519. PubMed PMID: 31310791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrocortisone loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles for topical ophthalmic administration: Preparation, characterization and evaluation of ophthalmic toxicity. AU - Álvarez-Álvarez,Lucía, AU - Barral,Luis, AU - Bouza,Rebeca, AU - Farrag,Yousof, AU - Otero-Espinar,Francisco, AU - Feijóo-Bandín,Sandra, AU - Lago,Francisca, Y1 - 2019/07/13/ PY - 2019/04/24/received PY - 2019/06/27/revised PY - 2019/07/12/accepted PY - 2019/7/17/pubmed PY - 2019/7/17/medline PY - 2019/7/17/entrez KW - Hydrocortisone KW - PHBV KW - Polymeric nanoparticles KW - Topical ophthalmic administration SP - 118519 EP - 118519 JF - International journal of pharmaceutics JO - Int J Pharm VL - 568 N2 - Poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles (PHBV-NPs) to encapsulate hydrocortisone (HC) for topical ophthalmic administration were prepared and characterized. The technique used to prepare the nanoparticles (NPs) was emulsification/solvent evaporation. The obtained size was 237.3 ± 2.7 nm, suitable for topical ocular administration. The obtained results for the entrapment efficiency were between 1 and 2.5% and for the drug loading were around 0.5%. The release behaviour of HC from the PHBV-NPs was also analyzed, adjusting this to a Higuchi kinetic model. For the new drug delivery system developed the ocular toxicity profile was determined by viability studies carried out on bovine keratocytes, by a Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) and by a Bovine Corneal Opacity and Permeability assay (BCOP). The obtained results concluded that the new system is no cytotoxic on bovine keratocytes and is neither irritating nor produces any alteration in the transparency and in the permeability of the cornea. Confocal studies were also performed and confirmed that PHBV-NPs are able to penetrate efficiently into the corneal tissue. This novel PHBV-based drug delivery system could be a good option for topical ophthalmic administration of drugs. SN - 1873-3476 UR - https://www.unboundmedicine.com/medline/citation/31310791/Hydrocortisone_loaded_poly-(3-hydroxybutyrate-co-3-hydroxyvalerate)_nanoparticles_for_topical_ophthalmic_administration:_Preparation,_characterization_and_evaluation_of_ophthalmic_toxicity L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(19)30563-0 DB - PRIME DP - Unbound Medicine ER -