Tags

Type your tag names separated by a space and hit enter

In-house rapid colorimetric method for detection of colistin resistance in Enterobacterales: A significant impact on resistance rates.
J Chemother 2019; :1-4JC

Abstract

A rapid colorimetric method, the Andrade Screening Antimicrobial Test (ASAT) was evaluated to detect colistin resistance in Enterobacteriales clinical isolates. The sensitivity and specificity were 90.7% and 100%, respectively. In 10/26 E. coli isolates the automatized method failed to detect the resistance, whereas the ASAT detected it accurately. Most of these isolates showed COL MIC values in the range 4-8 µg mL-1 and carried mcr-1. As regards K. pneumoniae COL- resistant isolates, discrepancies between the Phoenix system and the ASAT were observed only in 3/44 isolates, most of them carried the blaKPC gene and showed COL MIC values >16 µg mL-1.

Authors+Show Affiliations

a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.a Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica , Universidad de Buenos Aires. Buenos Aires, INFIBIOC, UBA , Argentina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31311461

Citation

Rodriguez, Carlos Hernán, et al. "In-house Rapid Colorimetric Method for Detection of Colistin Resistance in Enterobacterales: a Significant Impact On Resistance Rates." Journal of Chemotherapy (Florence, Italy), 2019, pp. 1-4.
Rodriguez CH, Maza J, Tamarin S, et al. In-house rapid colorimetric method for detection of colistin resistance in Enterobacterales: A significant impact on resistance rates. J Chemother. 2019.
Rodriguez, C. H., Maza, J., Tamarin, S., Nastro, M., Vay, C., & Famiglietti, A. (2019). In-house rapid colorimetric method for detection of colistin resistance in Enterobacterales: A significant impact on resistance rates. Journal of Chemotherapy (Florence, Italy), pp. 1-4. doi:10.1080/1120009X.2019.1637557.
Rodriguez CH, et al. In-house Rapid Colorimetric Method for Detection of Colistin Resistance in Enterobacterales: a Significant Impact On Resistance Rates. J Chemother. 2019 Jul 17;1-4. PubMed PMID: 31311461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-house rapid colorimetric method for detection of colistin resistance in Enterobacterales: A significant impact on resistance rates. AU - Rodriguez,Carlos Hernán, AU - Maza,Jonathan, AU - Tamarin,Samanta, AU - Nastro,Marcela, AU - Vay,Carlos, AU - Famiglietti,Angela, Y1 - 2019/07/17/ PY - 2019/7/18/entrez KW - ASAT test KW - categorical agreement KW - colistin KW - colistin-resistance KW - essential agreement KW - mcr KW - rapid test SP - 1 EP - 4 JF - Journal of chemotherapy (Florence, Italy) JO - J Chemother N2 - A rapid colorimetric method, the Andrade Screening Antimicrobial Test (ASAT) was evaluated to detect colistin resistance in Enterobacteriales clinical isolates. The sensitivity and specificity were 90.7% and 100%, respectively. In 10/26 E. coli isolates the automatized method failed to detect the resistance, whereas the ASAT detected it accurately. Most of these isolates showed COL MIC values in the range 4-8 µg mL-1 and carried mcr-1. As regards K. pneumoniae COL- resistant isolates, discrepancies between the Phoenix system and the ASAT were observed only in 3/44 isolates, most of them carried the blaKPC gene and showed COL MIC values >16 µg mL-1. SN - 1973-9478 UR - https://www.unboundmedicine.com/medline/citation/31311461/In-house_rapid_colorimetric_method_for_detection_of_colistin_resistance_in_Enterobacterales:_A_significant_impact_on_resistance_rates L2 - http://www.tandfonline.com/doi/full/10.1080/1120009X.2019.1637557 DB - PRIME DP - Unbound Medicine ER -