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Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers.
Theor Appl Genet. 2019 Oct; 132(10):2881-2898.TA

Abstract

KEY MESSAGE

Using COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat-A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P-6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.

Authors+Show Affiliations

Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic. Field Crops Research Institute, Agricultural Research Centre, 9 Gamma Street, Giza, Cairo, 12619, Egypt.Genetics Department, ETSIAM, Agrifood Campus of International Excellence (ceiA3), University of Córdoba, 14071, Córdoba, Spain.Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic. Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary.Genetics Department, ETSIAM, Agrifood Campus of International Excellence (ceiA3), University of Córdoba, 14071, Córdoba, Spain.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic.Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 78371, Olomouc, Czech Republic. vrana@ueb.cas.cz.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31312850

Citation

Said, Mahmoud, et al. "Uncovering Homeologous Relationships Between Tetraploid Agropyron Cristatum and Bread Wheat Genomes Using COS Markers." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 132, no. 10, 2019, pp. 2881-2898.
Said M, Parada AC, Gaál E, et al. Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers. Theor Appl Genet. 2019;132(10):2881-2898.
Said, M., Parada, A. C., Gaál, E., Molnár, I., Cabrera, A., Doležel, J., & Vrána, J. (2019). Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 132(10), 2881-2898. https://doi.org/10.1007/s00122-019-03394-1
Said M, et al. Uncovering Homeologous Relationships Between Tetraploid Agropyron Cristatum and Bread Wheat Genomes Using COS Markers. Theor Appl Genet. 2019;132(10):2881-2898. PubMed PMID: 31312850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers. AU - Said,Mahmoud, AU - Parada,Alejandro Copete, AU - Gaál,Eszter, AU - Molnár,István, AU - Cabrera,Adoración, AU - Doležel,Jaroslav, AU - Vrána,Jan, Y1 - 2019/07/16/ PY - 2018/10/09/received PY - 2019/07/06/accepted PY - 2019/7/18/pubmed PY - 2020/2/27/medline PY - 2019/7/18/entrez SP - 2881 EP - 2898 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 132 IS - 10 N2 - KEY MESSAGE: Using COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat-A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P-6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31312850/Uncovering_homeologous_relationships_between_tetraploid_Agropyron_cristatum_and_bread_wheat_genomes_using_COS_markers_ L2 - https://dx.doi.org/10.1007/s00122-019-03394-1 DB - PRIME DP - Unbound Medicine ER -