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Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY21 gene in resistance to abiotic stresses.
Genome 2019; 62(10):689-703G

Abstract

Sea buckthorn (Hippophae rhamnoides L.) is a plant with economic and ecological value. It is uniquely capable of growing well under salt and drought stress. WRKY transcription factors play important roles in the ability of plants to resist stress. In this study, 48 HrWRKY genes were identified based on RNA sequencing of H. rhamnoides. Evaluation of expression pattern of HrWRKY1, HrWRKY17, HrWRKY18, HrWRKY21, HrWRKY33-2, HrWRKY40-2, HrWRKY41, and HrWRKY71 suggested that they were involved in abiotic stress. Interestingly, HrWRKY21, one of eight HrWRKY genes, was a positive regulator of abiotic stress tolerance in H. rhamnoides. In addition, most morphological attributes of roots in transgenic Nicotiana tabacum lines (overexpressing HrWRKY21) were also markedly increased compared with the wild-type (WT), including total lengths, specific root lengths, and surface areas. Stress tolerance of transgenic lines was also correlated with higher antioxidant activity (SOD and POD), lower percentage of relative conductivity (REC), and lower activity of malondialdehyde (MDA) under stress conditions. These findings represent a foundation of knowledge about the molecular mechanisms driving resistance to adverse conditions in plants; they are a promising step towards development of tree cultivars with improved tolerance to abiotic stress.

Authors+Show Affiliations

College of Life Science, Hebei University, Baoding, China.College of Life Science, Hebei University, Baoding, China.College of Life Science, Hebei University, Baoding, China.Desert Forest Experimental Center, Chinese Academy of Forestry, Dengkou, China.College of Life Science, Hebei University, Baoding, China.College of Life Science, Hebei University, Baoding, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31315001

Citation

Wang, Zhaoyu, et al. "Characterization of the Hippophae Rhamnoides WRKY Gene Family and Functional Analysis of the Role of the HrWRKY21 Gene in Resistance to Abiotic Stresses." Genome, vol. 62, no. 10, 2019, pp. 689-703.
Wang Z, Feng R, Zhang X, et al. Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY21 gene in resistance to abiotic stresses. Genome. 2019;62(10):689-703.
Wang, Z., Feng, R., Zhang, X., Su, Z., Wei, J., & Liu, J. (2019). Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY21 gene in resistance to abiotic stresses. Genome, 62(10), pp. 689-703. doi:10.1139/gen-2019-0024.
Wang Z, et al. Characterization of the Hippophae Rhamnoides WRKY Gene Family and Functional Analysis of the Role of the HrWRKY21 Gene in Resistance to Abiotic Stresses. Genome. 2019;62(10):689-703. PubMed PMID: 31315001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY21 gene in resistance to abiotic stresses. AU - Wang,Zhaoyu, AU - Feng,Runxia, AU - Zhang,Xue, AU - Su,Zhi, AU - Wei,Jianrong, AU - Liu,Jianfeng, Y1 - 2019/07/17/ PY - 2019/7/18/pubmed PY - 2019/7/18/medline PY - 2019/7/18/entrez KW - HrWRKY21 transcription factor KW - abiotic stresses KW - argousier KW - facteur de transcription HrWRKY21 KW - overexpression KW - sea buckthorn KW - stress abiotiques KW - surexpression SP - 689 EP - 703 JF - Genome JO - Genome VL - 62 IS - 10 N2 - Sea buckthorn (Hippophae rhamnoides L.) is a plant with economic and ecological value. It is uniquely capable of growing well under salt and drought stress. WRKY transcription factors play important roles in the ability of plants to resist stress. In this study, 48 HrWRKY genes were identified based on RNA sequencing of H. rhamnoides. Evaluation of expression pattern of HrWRKY1, HrWRKY17, HrWRKY18, HrWRKY21, HrWRKY33-2, HrWRKY40-2, HrWRKY41, and HrWRKY71 suggested that they were involved in abiotic stress. Interestingly, HrWRKY21, one of eight HrWRKY genes, was a positive regulator of abiotic stress tolerance in H. rhamnoides. In addition, most morphological attributes of roots in transgenic Nicotiana tabacum lines (overexpressing HrWRKY21) were also markedly increased compared with the wild-type (WT), including total lengths, specific root lengths, and surface areas. Stress tolerance of transgenic lines was also correlated with higher antioxidant activity (SOD and POD), lower percentage of relative conductivity (REC), and lower activity of malondialdehyde (MDA) under stress conditions. These findings represent a foundation of knowledge about the molecular mechanisms driving resistance to adverse conditions in plants; they are a promising step towards development of tree cultivars with improved tolerance to abiotic stress. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/31315001/Characterization_of_the_Hippophae_rhamnoides_WRKY_gene_family_and_functional_analysis_of_the_role_of_the_HrWRKY_21_gene_in_resistance_to_abiotic_stresses L2 - http://www.nrcresearchpress.com/doi/full/10.1139/gen-2019-0024?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -