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Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY 21 gene in resistance to abiotic stresses.
Genome 2019G

Abstract

Seabuckthorn (Hippophae rhamnoides L.) is a plant with economic and ecological value. It is uniquely capable of growing well under salt and drought stress. WRKY transcription factors play important roles in the ability of plants to resist stress. In this study, 48 HrWRKY genes were identified based on RNA sequencing of H. rhamnoides. Evaluation of expression pattern of HrWRKY 1, HrWRKY 17, HrWRKY 18, HrWRKY 21, HrWRKY 33-2, HrWRKY 40-2, HrWRKY 41 and HrWRKY 71 suggested that they were involved in abiotic stress. Interestingly, HrWRKY 21, one of eight HrWRKY genes, was a positive regulator of abiotic stress tolerance in H. rhamnoides. In addition, most morphological attributes of roots in transgenic Nicotiana tabacum lines (overexpressing HrWRKY 21) were also markedly increased compared with the wild-type (WT), including total lengths, specific root lengths and surface areas. Stress tolerance of transgenic lines was also correlated with higher antioxidant activity (SOD and POD), lower percentage of relative conductivity (REC) and lower activity of malondialdehyde (MDA) under stress conditions. These findings represent a foundation of knowledge about the molecular mechanisms driving resistance to adverse conditions in plants; they are a promising step towards development of tree cultivars with improved tolerance to abiotic stress.

Authors+Show Affiliations

Hebei University, 56667, Baoding, Hebei, China ; zhaoyuwang1105@163.com.Hebei University, 56667, Baoding, Hebei, China ; 2292751960@qq.com.Hebei University, 56667, Baoding, Hebei, China ; 772597541@qq.com.Chinese Academy of Forestry, 74640, Haidian District, Beijing, China ; dksuzhi@sina.com.Hebei University, 56667, Baoding, Hebei, China ; jrwei9@126.com.Hebei University, 56667, Baoding, Hebei, China ; jianfengliu@hbu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31315001

Citation

Wang, Zhaoyu, et al. "Characterization of the Hippophae Rhamnoides WRKY Gene Family and Functional Analysis of the Role of the HrWRKY 21 Gene in Resistance to Abiotic Stresses." Genome, 2019.
Wang Z, Feng R, Zhang X, et al. Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY 21 gene in resistance to abiotic stresses. Genome. 2019.
Wang, Z., Feng, R., Zhang, X., Su, Z., Wei, J., & Liu, J. (2019). Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY 21 gene in resistance to abiotic stresses. Genome, doi:10.1139/gen-2019-0024.
Wang Z, et al. Characterization of the Hippophae Rhamnoides WRKY Gene Family and Functional Analysis of the Role of the HrWRKY 21 Gene in Resistance to Abiotic Stresses. Genome. 2019 Jul 17; PubMed PMID: 31315001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the Hippophae rhamnoides WRKY gene family and functional analysis of the role of the HrWRKY 21 gene in resistance to abiotic stresses. AU - Wang,Zhaoyu, AU - Feng,Runxia, AU - Zhang,Xue, AU - Su,Zhi, AU - Wei,Jianrong, AU - Liu,Jianfeng, Y1 - 2019/07/17/ PY - 2019/7/18/entrez PY - 2019/7/18/pubmed PY - 2019/7/18/medline JF - Genome JO - Genome N2 - Seabuckthorn (Hippophae rhamnoides L.) is a plant with economic and ecological value. It is uniquely capable of growing well under salt and drought stress. WRKY transcription factors play important roles in the ability of plants to resist stress. In this study, 48 HrWRKY genes were identified based on RNA sequencing of H. rhamnoides. Evaluation of expression pattern of HrWRKY 1, HrWRKY 17, HrWRKY 18, HrWRKY 21, HrWRKY 33-2, HrWRKY 40-2, HrWRKY 41 and HrWRKY 71 suggested that they were involved in abiotic stress. Interestingly, HrWRKY 21, one of eight HrWRKY genes, was a positive regulator of abiotic stress tolerance in H. rhamnoides. In addition, most morphological attributes of roots in transgenic Nicotiana tabacum lines (overexpressing HrWRKY 21) were also markedly increased compared with the wild-type (WT), including total lengths, specific root lengths and surface areas. Stress tolerance of transgenic lines was also correlated with higher antioxidant activity (SOD and POD), lower percentage of relative conductivity (REC) and lower activity of malondialdehyde (MDA) under stress conditions. These findings represent a foundation of knowledge about the molecular mechanisms driving resistance to adverse conditions in plants; they are a promising step towards development of tree cultivars with improved tolerance to abiotic stress. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/31315001/Characterization_of_the_Hippophae_rhamnoides_WRKY_gene_family_and_functional_analysis_of_the_role_of_the_HrWRKY_21_gene_in_resistance_to_abiotic_stresses L2 - http://www.nrcresearchpress.com/doi/full/10.1139/gen-2019-0024?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -