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Transcriptome profiling of Puccinellia tenuiflora during seed germination under a long-term saline-alkali stress.
BMC Genomics 2019; 20(1):589BG

Abstract

BACKGROUND

Puccinellia tenuiflora is the most saline-alkali tolerant plant in the Songnen Plain, one of the three largest soda saline-alkali lands worldwide. Here, we investigated the physicochemical properties of saline-alkali soils from the Songnen Plain and sequenced the transcriptomes of germinated P. tenuiflora seedlings under long-term treatment (from seed soaking) with saline-alkali soil extracts.

RESULTS

We found that the soils from Songnen Plain were reasonably rich in salts and alkali; moreover, the soils were severely deficient in nitrogen [N], phosphorus [P], potassium [K] and several other mineral elements. This finding demonstrated that P. tenuiflora can survive from not only high saline-alkali stress but also a lack of essential mineral elements. To explore the saline-alkali tolerance mechanism, transcriptional analyses of P. tenuiflora plants treated with water extracts from the saline-alkali soils was performed. Interestingly, unigenes involved in the uptake of N, P, K and the micronutrients were found to be significantly upregulated, which indicated the existence of an efficient nutrition-uptake system in P. tenuiflora. Compared with P. tenuiflora, the rice Oryza sativa was hypersensitive to saline-alkali stress. The results obtained using a noninvasive microtest techniques confirmed that the uptake of NO3- and NH4+ and the regulatory flux of Na+ and H+ were significantly higher in the roots of P. tenuiflora than in those of O. sativa. In the corresponding physiological experiments, the application of additional nutrition elements significantly eliminated the sensitive symptoms of rice to saline-alkali soil extracts.

CONCLUSIONS

Our results imply that the survival of P. tenuiflora in saline-alkali soils is due to a combination of at least two regulatory mechanisms and the high nutrient uptake capacity of P. tenuiflora plays a pivotal role in its adaptation to those stress. Taken together, our results highlight the role of nutrition uptake in saline-alkali stress tolerance in plants.

Authors+Show Affiliations

Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China.Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China.Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China.Asian Natural Environmental Science Center (ANESC), the University of Tokyo, Nishitokyo-shi, Tokyo, 188-0002, Japan.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Hangzhou, 311300, China. shenkuiliu@nefu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31315555

Citation

Ye, Xiaoxue, et al. "Transcriptome Profiling of Puccinellia Tenuiflora During Seed Germination Under a Long-term Saline-alkali Stress." BMC Genomics, vol. 20, no. 1, 2019, p. 589.
Ye X, Wang H, Cao X, et al. Transcriptome profiling of Puccinellia tenuiflora during seed germination under a long-term saline-alkali stress. BMC Genomics. 2019;20(1):589.
Ye, X., Wang, H., Cao, X., Jin, X., Cui, F., Bu, Y., ... Liu, S. (2019). Transcriptome profiling of Puccinellia tenuiflora during seed germination under a long-term saline-alkali stress. BMC Genomics, 20(1), p. 589. doi:10.1186/s12864-019-5860-5.
Ye X, et al. Transcriptome Profiling of Puccinellia Tenuiflora During Seed Germination Under a Long-term Saline-alkali Stress. BMC Genomics. 2019 Jul 17;20(1):589. PubMed PMID: 31315555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome profiling of Puccinellia tenuiflora during seed germination under a long-term saline-alkali stress. AU - Ye,Xiaoxue, AU - Wang,Hao, AU - Cao,Xiuling, AU - Jin,Xuejiao, AU - Cui,Fuqiang, AU - Bu,Yuanyuan, AU - Liu,Hua, AU - Wu,Wenwu, AU - Takano,Tetsuo, AU - Liu,Shenkui, Y1 - 2019/07/17/ PY - 2018/11/13/received PY - 2019/05/29/accepted PY - 2019/7/19/entrez PY - 2019/7/19/pubmed PY - 2019/7/19/medline KW - Puccinellia tenuiflora KW - abiotic stress KW - mineral elements KW - noninvasive microtest technique KW - saline-alkali KW - transcriptome SP - 589 EP - 589 JF - BMC genomics JO - BMC Genomics VL - 20 IS - 1 N2 - BACKGROUND: Puccinellia tenuiflora is the most saline-alkali tolerant plant in the Songnen Plain, one of the three largest soda saline-alkali lands worldwide. Here, we investigated the physicochemical properties of saline-alkali soils from the Songnen Plain and sequenced the transcriptomes of germinated P. tenuiflora seedlings under long-term treatment (from seed soaking) with saline-alkali soil extracts. RESULTS: We found that the soils from Songnen Plain were reasonably rich in salts and alkali; moreover, the soils were severely deficient in nitrogen [N], phosphorus [P], potassium [K] and several other mineral elements. This finding demonstrated that P. tenuiflora can survive from not only high saline-alkali stress but also a lack of essential mineral elements. To explore the saline-alkali tolerance mechanism, transcriptional analyses of P. tenuiflora plants treated with water extracts from the saline-alkali soils was performed. Interestingly, unigenes involved in the uptake of N, P, K and the micronutrients were found to be significantly upregulated, which indicated the existence of an efficient nutrition-uptake system in P. tenuiflora. Compared with P. tenuiflora, the rice Oryza sativa was hypersensitive to saline-alkali stress. The results obtained using a noninvasive microtest techniques confirmed that the uptake of NO3- and NH4+ and the regulatory flux of Na+ and H+ were significantly higher in the roots of P. tenuiflora than in those of O. sativa. In the corresponding physiological experiments, the application of additional nutrition elements significantly eliminated the sensitive symptoms of rice to saline-alkali soil extracts. CONCLUSIONS: Our results imply that the survival of P. tenuiflora in saline-alkali soils is due to a combination of at least two regulatory mechanisms and the high nutrient uptake capacity of P. tenuiflora plays a pivotal role in its adaptation to those stress. Taken together, our results highlight the role of nutrition uptake in saline-alkali stress tolerance in plants. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/31315555/Transcriptome_profiling_of_Puccinellia_tenuiflora_during_seed_germination_under_a_long-term_saline-alkali_stress L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5860-5 DB - PRIME DP - Unbound Medicine ER -