Tags

Type your tag names separated by a space and hit enter

The extract from Agkistrodon halys venom protects against lipopolysaccharide (LPS)-induced myocardial injury.
BMC Complement Altern Med. 2019 Jul 17; 19(1):176.BC

Abstract

BACKGROUND

Snake venoms contain various bioactive constituents which possess potential therapeutic effects. The aim of this work was to investigate the effect of the extract from Agkistrodon halys venom on lipopolysaccharide (LPS)-induced myocardial injury.

METHODS

Thirty male Sprague-Dawley rats were randomly assigned to three groups (10 rats per group): control group, LPS group and LPS + extract group. Rats in control and the LPS groups were intravenously injected with sterile saline solution, and rats in the LPS + extract group with the extract. After 2 h, rats of the control group were intraperitoneally injected sterile saline solution, and rats in the LPS and the LPS + extract groups were treated with LPS (20 mg per kg body weight). Levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum were determined. Anti-inflammation of the extract was analyzed via determination of TNF-α and IL-6 in serum, and expression of TNF-α, IL-6, COX-2 and p-ERK protein in hearts. Heme oxygenase-1 (HO-1) and p-NF-κB protein expression in hearts, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in serum were used to evaluate the anti-oxidative properties of the extract.

RESULTS

Extract pretreatment significantly decreased the level of serum CK and LDH, reduced the generation of inflammatory cytokines such as TNF-α and IL-6, and also reduced serum level of MDA in the LPS + extract group compared with the LPS group. In addition, the extract increased SOD activity in serum, HO-1 protein expression in hearts, and decreased TNF-α, IL-6, COX-2, p-NF-κB and p-ERK1/2 protein expression.

CONCLUSION

Our results suggested that beneficial effect of this extract might be associated with an improved anti-oxidation and anti-inflammatory effect via downregulation of NF-κB/COX-2 signaling by activating HO-1/CO in hearts.

Authors+Show Affiliations

Department of Histology and Embryology, Wannan Medical College, Wuhu, 241002, People's Republic of China.Department of Pathophysiology, Wannan Medical College, 22 West Wenchang Road, Yijiang District, Wuhu, 241002, China.Department of Physiology, Wannan Medical College, Wuhu, 241002, People's Republic of China.Department of Pathophysiology, Wannan Medical College, 22 West Wenchang Road, Yijiang District, Wuhu, 241002, China.Department of Pathophysiology, Wannan Medical College, 22 West Wenchang Road, Yijiang District, Wuhu, 241002, China. guoguangw1226@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31315617

Citation

Wang, Quan-Hai, et al. "The Extract From Agkistrodon Halys Venom Protects Against Lipopolysaccharide (LPS)-induced Myocardial Injury." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 176.
Wang QH, Li W, Jiang YX, et al. The extract from Agkistrodon halys venom protects against lipopolysaccharide (LPS)-induced myocardial injury. BMC Complement Altern Med. 2019;19(1):176.
Wang, Q. H., Li, W., Jiang, Y. X., Lu, X. H., & Wang, G. G. (2019). The extract from Agkistrodon halys venom protects against lipopolysaccharide (LPS)-induced myocardial injury. BMC Complementary and Alternative Medicine, 19(1), 176. https://doi.org/10.1186/s12906-019-2595-4
Wang QH, et al. The Extract From Agkistrodon Halys Venom Protects Against Lipopolysaccharide (LPS)-induced Myocardial Injury. BMC Complement Altern Med. 2019 Jul 17;19(1):176. PubMed PMID: 31315617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The extract from Agkistrodon halys venom protects against lipopolysaccharide (LPS)-induced myocardial injury. AU - Wang,Quan-Hai, AU - Li,Wei, AU - Jiang,Yu-Xin, AU - Lu,Xiao-Hua, AU - Wang,Guo-Guang, Y1 - 2019/07/17/ PY - 2017/03/22/received PY - 2019/07/09/accepted PY - 2019/7/19/entrez PY - 2019/7/19/pubmed PY - 2019/7/19/medline KW - Agkistrodon halys venom KW - LPS KW - Myocardial injury SP - 176 EP - 176 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Snake venoms contain various bioactive constituents which possess potential therapeutic effects. The aim of this work was to investigate the effect of the extract from Agkistrodon halys venom on lipopolysaccharide (LPS)-induced myocardial injury. METHODS: Thirty male Sprague-Dawley rats were randomly assigned to three groups (10 rats per group): control group, LPS group and LPS + extract group. Rats in control and the LPS groups were intravenously injected with sterile saline solution, and rats in the LPS + extract group with the extract. After 2 h, rats of the control group were intraperitoneally injected sterile saline solution, and rats in the LPS and the LPS + extract groups were treated with LPS (20 mg per kg body weight). Levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum were determined. Anti-inflammation of the extract was analyzed via determination of TNF-α and IL-6 in serum, and expression of TNF-α, IL-6, COX-2 and p-ERK protein in hearts. Heme oxygenase-1 (HO-1) and p-NF-κB protein expression in hearts, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in serum were used to evaluate the anti-oxidative properties of the extract. RESULTS: Extract pretreatment significantly decreased the level of serum CK and LDH, reduced the generation of inflammatory cytokines such as TNF-α and IL-6, and also reduced serum level of MDA in the LPS + extract group compared with the LPS group. In addition, the extract increased SOD activity in serum, HO-1 protein expression in hearts, and decreased TNF-α, IL-6, COX-2, p-NF-κB and p-ERK1/2 protein expression. CONCLUSION: Our results suggested that beneficial effect of this extract might be associated with an improved anti-oxidation and anti-inflammatory effect via downregulation of NF-κB/COX-2 signaling by activating HO-1/CO in hearts. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31315617/The_extract_from_Agkistrodon_halys_venom_protects_against_lipopolysaccharide_(LPS)-induced_myocardial_injury L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2595-4 DB - PRIME DP - Unbound Medicine ER -