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A direct interaction between survivin and myosin II is required for cytokinesis.
J Cell Sci 2019; 132(14)JC

Abstract

An acto-myosin contractile ring, which forms after anaphase onset and is highly regulated in time and space, mediates cytokinesis, the final step of mitosis. The chromosomal passenger complex (CPC), composed of Aurora-B kinase, INCENP, borealin and survivin (also known as BIRC5), regulates various processes during mitosis, including cytokinesis. It is not understood, however, how CPC regulates cytokinesis. We show that survivin binds to non-muscle myosin II (NMII), regulating its filament assembly. Survivin and NMII interact mainly in telophase, and Cdk1 regulates their interaction in a mitotic-phase-specific manner, revealing the mechanism for the specific timing of survivin-NMII interaction during mitosis. The survivin-NMII interaction is indispensable for cytokinesis, and its disruption leads to multiple mitotic defects. We further show that only the survivin homodimer binds to NMII, attesting to the biological importance for survivin homodimerization. We suggest a novel function for survivin in regulating the spatio-temporal formation of the acto-NMII contractile ring during cytokinesis and we elucidate the role of Cdk1 in regulating this process.This article has an associated First Person interview with the first author of the paper.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.Department of Biochemistry and Molecular Biology, The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel shoshr@ekmd.huji.ac.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31315909

Citation

Babkoff, Aryeh, et al. "A Direct Interaction Between Survivin and Myosin II Is Required for Cytokinesis." Journal of Cell Science, vol. 132, no. 14, 2019.
Babkoff A, Cohen-Kfir E, Aharon H, et al. A direct interaction between survivin and myosin II is required for cytokinesis. J Cell Sci. 2019;132(14).
Babkoff, A., Cohen-Kfir, E., Aharon, H., Ronen, D., Rosenberg, M., Wiener, R., & Ravid, S. (2019). A direct interaction between survivin and myosin II is required for cytokinesis. Journal of Cell Science, 132(14), doi:10.1242/jcs.233130.
Babkoff A, et al. A Direct Interaction Between Survivin and Myosin II Is Required for Cytokinesis. J Cell Sci. 2019 Jul 17;132(14) PubMed PMID: 31315909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A direct interaction between survivin and myosin II is required for cytokinesis. AU - Babkoff,Aryeh, AU - Cohen-Kfir,Einav, AU - Aharon,Hananel, AU - Ronen,Daniel, AU - Rosenberg,Michael, AU - Wiener,Reuven, AU - Ravid,Shoshana, Y1 - 2019/07/17/ PY - 2019/04/10/received PY - 2019/06/14/accepted PY - 2019/7/19/entrez KW - Cytokinesis KW - Non-muscle myosin II KW - Survivin JF - Journal of cell science JO - J. Cell. Sci. VL - 132 IS - 14 N2 - An acto-myosin contractile ring, which forms after anaphase onset and is highly regulated in time and space, mediates cytokinesis, the final step of mitosis. The chromosomal passenger complex (CPC), composed of Aurora-B kinase, INCENP, borealin and survivin (also known as BIRC5), regulates various processes during mitosis, including cytokinesis. It is not understood, however, how CPC regulates cytokinesis. We show that survivin binds to non-muscle myosin II (NMII), regulating its filament assembly. Survivin and NMII interact mainly in telophase, and Cdk1 regulates their interaction in a mitotic-phase-specific manner, revealing the mechanism for the specific timing of survivin-NMII interaction during mitosis. The survivin-NMII interaction is indispensable for cytokinesis, and its disruption leads to multiple mitotic defects. We further show that only the survivin homodimer binds to NMII, attesting to the biological importance for survivin homodimerization. We suggest a novel function for survivin in regulating the spatio-temporal formation of the acto-NMII contractile ring during cytokinesis and we elucidate the role of Cdk1 in regulating this process.This article has an associated First Person interview with the first author of the paper. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/31315909/A_direct_interaction_between_survivin_and_myosin_II_is_required_for_cytokinesis L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=31315909 DB - PRIME DP - Unbound Medicine ER -