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A calmodulin-gated calcium channel links pathogen patterns to plant immunity.
Nature 2019; 572(7767):131-135Nat

Abstract

Pathogen-associated molecular patterns (PAMPs) activate innate immunity in both animals and plants. Although calcium has long been recognized as an essential signal for PAMP-triggered immunity in plants, the mechanism of PAMP-induced calcium signalling remains unknown1,2. Here we report that calcium nutrient status is critical for calcium-dependent PAMP-triggered immunity in plants. When calcium supply is sufficient, two genes that encode cyclic nucleotide-gated channel (CNGC) proteins, CNGC2 and CNGC4, are essential for PAMP-induced calcium signalling in Arabidopsis3-7. In a reconstitution system, we find that the CNGC2 and CNGC4 proteins together-but neither alone-assemble into a functional calcium channel that is blocked by calmodulin in the resting state. Upon pathogen attack, the channel is phosphorylated and activated by the effector kinase BOTRYTIS-INDUCED KINASE1 (BIK1) of the pattern-recognition receptor complex, and this triggers an increase in the concentration of cytosolic calcium8-10. The CNGC-mediated calcium entry thus provides a critical link between the pattern-recognition receptor complex and calcium-dependent immunity programs in the PAMP-triggered immunity signalling pathway in plants.

Authors+Show Affiliations

Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.College of Life Sciences, Capital Normal University, Beijing, China.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology, College of Life Sciences, Nanjing University, Nanjing, China.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.College of Life Sciences, Capital Normal University, Beijing, China.College of Life Sciences, Capital Normal University, Beijing, China.College of Life Sciences, Capital Normal University, Beijing, China.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA. sluan@berkeley.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31316205

Citation

Tian, Wang, et al. "A Calmodulin-gated Calcium Channel Links Pathogen Patterns to Plant Immunity." Nature, vol. 572, no. 7767, 2019, pp. 131-135.
Tian W, Hou C, Ren Z, et al. A calmodulin-gated calcium channel links pathogen patterns to plant immunity. Nature. 2019;572(7767):131-135.
Tian, W., Hou, C., Ren, Z., Wang, C., Zhao, F., Dahlbeck, D., ... Luan, S. (2019). A calmodulin-gated calcium channel links pathogen patterns to plant immunity. Nature, 572(7767), pp. 131-135. doi:10.1038/s41586-019-1413-y.
Tian W, et al. A Calmodulin-gated Calcium Channel Links Pathogen Patterns to Plant Immunity. Nature. 2019;572(7767):131-135. PubMed PMID: 31316205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A calmodulin-gated calcium channel links pathogen patterns to plant immunity. AU - Tian,Wang, AU - Hou,Congcong, AU - Ren,Zhijie, AU - Wang,Chao, AU - Zhao,Fugeng, AU - Dahlbeck,Douglas, AU - Hu,Songping, AU - Zhang,Liying, AU - Niu,Qi, AU - Li,Legong, AU - Staskawicz,Brian J, AU - Luan,Sheng, Y1 - 2019/07/17/ PY - 2017/12/30/received PY - 2019/06/25/accepted PY - 2019/7/19/pubmed PY - 2019/7/19/medline PY - 2019/7/19/entrez SP - 131 EP - 135 JF - Nature JO - Nature VL - 572 IS - 7767 N2 - Pathogen-associated molecular patterns (PAMPs) activate innate immunity in both animals and plants. Although calcium has long been recognized as an essential signal for PAMP-triggered immunity in plants, the mechanism of PAMP-induced calcium signalling remains unknown1,2. Here we report that calcium nutrient status is critical for calcium-dependent PAMP-triggered immunity in plants. When calcium supply is sufficient, two genes that encode cyclic nucleotide-gated channel (CNGC) proteins, CNGC2 and CNGC4, are essential for PAMP-induced calcium signalling in Arabidopsis3-7. In a reconstitution system, we find that the CNGC2 and CNGC4 proteins together-but neither alone-assemble into a functional calcium channel that is blocked by calmodulin in the resting state. Upon pathogen attack, the channel is phosphorylated and activated by the effector kinase BOTRYTIS-INDUCED KINASE1 (BIK1) of the pattern-recognition receptor complex, and this triggers an increase in the concentration of cytosolic calcium8-10. The CNGC-mediated calcium entry thus provides a critical link between the pattern-recognition receptor complex and calcium-dependent immunity programs in the PAMP-triggered immunity signalling pathway in plants. SN - 1476-4687 UR - https://www.unboundmedicine.com/medline/citation/31316205/A_calmodulin-gated_calcium_channel_links_pathogen_patterns_to_plant_immunity L2 - https://doi.org/10.1038/s41586-019-1413-y DB - PRIME DP - Unbound Medicine ER -