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Development of a bio-MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair.

Abstract

Here we propose a bio-MEMS device designed to evaluate contractile force and conduction velocity of cell sheets in response to mechanical and electrical stimulation of the cell source as it grows to form a cellular sheet. Moreover, the design allows for the incorporation of patient-specific data and cell sources. An optimized device would allow cell sheets to be cultured, characterized, and conditioned to be compatible with a specific patient's cardiac environment in vitro, before implantation. This design draws upon existing methods in the literature but makes an important advance by combining the mechanical and electrical stimulation into a single system for optimized cell sheet growth. The device has been designed to achieve cellular alignment, electrical stimulation, mechanical stimulation, conduction velocity readout, contraction force readout, and eventually cell sheet release. The platform is a set of comb electrical contacts consisting of three-dimensional walls made of polydimethylsiloxane and coated with electrically conductive metals on the tops of the walls. Not only do the walls serve as a method for stimulating cells that are attached to the top, but their geometry is tailored such that they are flexible enough to be bent by the cells and used to measure force. The platform can be stretched via a linear actuator setup, allowing for simultaneous electrical and mechanical stimulation that can be derived from patient-specific clinical data.

Authors+Show Affiliations

Division of Materials Science and Engineering, Boston University, Boston, Massachusetts. Department of Cardiac Surgery, Boston Children's Hospital, Boston, Massachusetts.Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts.Department of Applied Physics and Materials Science, California Institute of Technology, Pasadena, California.Department of Biomedical Engineering, Boston University, Boston, Massachusetts.Harvard Medical School, Massachusetts General Hospital, Cardiovascular Research Center, Boston, Massachusetts.Harvard Medical School, Massachusetts General Hospital, Cardiovascular Research Center, Boston, Massachusetts.Department of Cardiac Surgery, Boston Children's Hospital, Boston, Massachusetts.Division of Materials Science and Engineering, Boston University, Boston, Massachusetts. Department of Biomedical Engineering, Boston University, Boston, Massachusetts.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31317531

Citation

Roberts, Erin G., et al. "Development of a bio-MEMS Device for Electrical and Mechanical Conditioning and Characterization of Cell Sheets for Myocardial Repair." Biotechnology and Bioengineering, 2019.
Roberts EG, Kleptsyn VF, Roberts GD, et al. Development of a bio-MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair. Biotechnol Bioeng. 2019.
Roberts, E. G., Kleptsyn, V. F., Roberts, G. D., Mossburg, K. J., Feng, B., Domian, I. J., ... Wong, J. Y. (2019). Development of a bio-MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair. Biotechnology and Bioengineering, doi:10.1002/bit.27123.
Roberts EG, et al. Development of a bio-MEMS Device for Electrical and Mechanical Conditioning and Characterization of Cell Sheets for Myocardial Repair. Biotechnol Bioeng. 2019 Jul 18; PubMed PMID: 31317531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a bio-MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair. AU - Roberts,Erin G, AU - Kleptsyn,Vladimir F, AU - Roberts,Gregory D, AU - Mossburg,Katherine J, AU - Feng,Bei, AU - Domian,Ibrahim J, AU - Emani,Sitaram M, AU - Wong,Joyce Y, Y1 - 2019/07/18/ PY - 2019/01/26/received PY - 2019/05/09/revised PY - 2019/07/09/accepted PY - 2019/7/19/pubmed PY - 2019/7/19/medline PY - 2019/7/19/entrez KW - beat contractility KW - bio-MEMS KW - conduction velocity KW - electromechanical stimulation KW - myocardium JF - Biotechnology and bioengineering JO - Biotechnol. Bioeng. N2 - Here we propose a bio-MEMS device designed to evaluate contractile force and conduction velocity of cell sheets in response to mechanical and electrical stimulation of the cell source as it grows to form a cellular sheet. Moreover, the design allows for the incorporation of patient-specific data and cell sources. An optimized device would allow cell sheets to be cultured, characterized, and conditioned to be compatible with a specific patient's cardiac environment in vitro, before implantation. This design draws upon existing methods in the literature but makes an important advance by combining the mechanical and electrical stimulation into a single system for optimized cell sheet growth. The device has been designed to achieve cellular alignment, electrical stimulation, mechanical stimulation, conduction velocity readout, contraction force readout, and eventually cell sheet release. The platform is a set of comb electrical contacts consisting of three-dimensional walls made of polydimethylsiloxane and coated with electrically conductive metals on the tops of the walls. Not only do the walls serve as a method for stimulating cells that are attached to the top, but their geometry is tailored such that they are flexible enough to be bent by the cells and used to measure force. The platform can be stretched via a linear actuator setup, allowing for simultaneous electrical and mechanical stimulation that can be derived from patient-specific clinical data. SN - 1097-0290 UR - https://www.unboundmedicine.com/medline/citation/31317531/Development_of_a_bio-MEMS_Device_for_Electrical_and_Mechanical_Conditioning_and_Characterization_of_Cell_Sheets_for_Myocardial_Repair L2 - https://doi.org/10.1002/bit.27123 DB - PRIME DP - Unbound Medicine ER -