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Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorus concentrations.
J Phycol 2019JP

Abstract

A novel freshwater strain of Coccomyxa elongata (MZ-Ch64) was isolated from the Zaporizhia region, Ukraine. The identification is based on the phylogenetic analysis of SSU rDNA gene and ITS1-5.8S rDNA-ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis places this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ-Ch64 formed a strongly supported lineage closest with C. elongata. The MZ-Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus-depleted conditions, was oleic acid (to 48.0-54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7-25.6%), cis-7-hexadecenoic acid (to 14.8%) and α-linolenic acid (to 9.1-10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9-54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis-7-hexadecenoic and α-linolenic acids for biotechnological applications. This article is protected by copyright. All rights reserved.

Authors+Show Affiliations

К.A. Timiryazev Institute of Plant Physiology RAS, IPP RAS, Moscow, 127276, Russia.Bohdan Khmelnytskyi Melitopol State Pedagogical University, 72312, Melitopol, Ukraine.К.A. Timiryazev Institute of Plant Physiology RAS, IPP RAS, Moscow, 127276, Russia.Museum of Natural History and Department of Ecology and Evolutionary Biology, University of Colorado, 80309, Boulder, Colorado, USA.К.A. Timiryazev Institute of Plant Physiology RAS, IPP RAS, Moscow, 127276, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31318981

Citation

Maltsev, Yevhen, et al. "Fatty Acid Content and Profile of the Novel Strain of Coccomyxa Elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations." Journal of Phycology, 2019.
Maltsev Y, Maltseva I, Maltseva S, et al. Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorus concentrations. J Phycol. 2019.
Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., & Kulikovskiy, M. (2019). Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorus concentrations. Journal of Phycology, doi:10.1111/jpy.12903.
Maltsev Y, et al. Fatty Acid Content and Profile of the Novel Strain of Coccomyxa Elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations. J Phycol. 2019 Jul 18; PubMed PMID: 31318981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorus concentrations. AU - Maltsev,Yevhen, AU - Maltseva,Irina, AU - Maltseva,Svetlana, AU - Kociolek,John Patrick, AU - Kulikovskiy,Maxim, Y1 - 2019/07/18/ PY - 2019/7/19/entrez PY - 2019/7/19/pubmed PY - 2019/7/19/medline KW - Coccomyxa KW - Trebouxiophyceae KW - fatty acid profile KW - lipid KW - nitrogen and phosphorus deprivation JF - Journal of phycology JO - J. Phycol. N2 - A novel freshwater strain of Coccomyxa elongata (MZ-Ch64) was isolated from the Zaporizhia region, Ukraine. The identification is based on the phylogenetic analysis of SSU rDNA gene and ITS1-5.8S rDNA-ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis places this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ-Ch64 formed a strongly supported lineage closest with C. elongata. The MZ-Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus-depleted conditions, was oleic acid (to 48.0-54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7-25.6%), cis-7-hexadecenoic acid (to 14.8%) and α-linolenic acid (to 9.1-10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9-54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis-7-hexadecenoic and α-linolenic acids for biotechnological applications. This article is protected by copyright. All rights reserved. SN - 1529-8817 UR - https://www.unboundmedicine.com/medline/citation/31318981/Fatty_acid_content_and_profile_of_the_novel_strain_of_Coccomyxa_elongata_(Trebouxiophyceae,_Chlorophyta)_cultivated_at_reduced_nitrogen_and_phosphorus_concentrations L2 - https://doi.org/10.1111/jpy.12903 DB - PRIME DP - Unbound Medicine ER -