Tags

Type your tag names separated by a space and hit enter

Site-Specific Incorporation of a Dithiolane Containing Amino Acid into Proteins.

Abstract

We have genetically encoded a dithiolane containing amino acid (dtF) in Escherichia coli (E. coli) using a polyspecific aminoacyl-tRNA synthetase (aaRS)/amber suppressor tRNA pair. To demonstrate the utility of dtF for bioapplications, we synthesized gold nanoparticle (AuNP) constructs with a mutant superfolder green fluorescent protein (sfGFP) [sfGFP-AuNP] as a model for the protein-metal conjugation. The resulting sfGFP-AuNP constructs show directional homogeneity and enhanced chemical durability compared to their cysteine analogues toward excess environmental 1,4-dithiothreitol (DTT).

Authors+Show Affiliations

Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Chemical Biology , Rutgers, The State University of New Jersey , Piscataway , New Jersey 08854 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.Department of Chemistry and Chemical Biology , Rutgers, The State University of New Jersey , Piscataway , New Jersey 08854 , United States. Department of Life and Nanopharmaceutical Science, College of Pharmacy , Kyung Hee University , Seoul 02447 , Republic of Korea.Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31319026

Citation

Koh, Minseob, et al. "Site-Specific Incorporation of a Dithiolane Containing Amino Acid Into Proteins." Bioconjugate Chemistry, 2019.
Koh M, Cho HY, Yu C, et al. Site-Specific Incorporation of a Dithiolane Containing Amino Acid into Proteins. Bioconjug Chem. 2019.
Koh, M., Cho, H. Y., Yu, C., Choi, S., Lee, K. B., & Schultz, P. G. (2019). Site-Specific Incorporation of a Dithiolane Containing Amino Acid into Proteins. Bioconjugate Chemistry, doi:10.1021/acs.bioconjchem.9b00413.
Koh M, et al. Site-Specific Incorporation of a Dithiolane Containing Amino Acid Into Proteins. Bioconjug Chem. 2019 Jul 23; PubMed PMID: 31319026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Site-Specific Incorporation of a Dithiolane Containing Amino Acid into Proteins. AU - Koh,Minseob, AU - Cho,Hyeon-Yeol, AU - Yu,Chenguang, AU - Choi,Seihyun, AU - Lee,Ki-Bum, AU - Schultz,Peter G, Y1 - 2019/07/23/ PY - 2019/7/19/pubmed PY - 2019/7/19/medline PY - 2019/7/19/entrez JF - Bioconjugate chemistry JO - Bioconjug. Chem. N2 - We have genetically encoded a dithiolane containing amino acid (dtF) in Escherichia coli (E. coli) using a polyspecific aminoacyl-tRNA synthetase (aaRS)/amber suppressor tRNA pair. To demonstrate the utility of dtF for bioapplications, we synthesized gold nanoparticle (AuNP) constructs with a mutant superfolder green fluorescent protein (sfGFP) [sfGFP-AuNP] as a model for the protein-metal conjugation. The resulting sfGFP-AuNP constructs show directional homogeneity and enhanced chemical durability compared to their cysteine analogues toward excess environmental 1,4-dithiothreitol (DTT). SN - 1520-4812 UR - https://www.unboundmedicine.com/medline/citation/31319026/Site-specific_Incorporation_of_a_Dithiolane_Containing_Amino_Acid_into_Proteins L2 - https://dx.doi.org/10.1021/acs.bioconjchem.9b00413 DB - PRIME DP - Unbound Medicine ER -