Tags

Type your tag names separated by a space and hit enter

Exploring the enzymatic degradation of poly(glycerol adipate).
Eur J Pharm Biopharm. 2019 Sep; 142:377-386.EJ

Abstract

Poly(glycerol adipate) (PGA) is a biodegradable, biocompatible, polymer with a great deal of potential in the field of drug delivery. Active drug molecules can be conjugated to the polymer backbone or encapsulated in self-assembled nanoparticles for targeted and systemic delivery. Here, a range of techniques have been used to characterise the enzymatic degradation of PGA extensively for the first time and to provide an indication of the way the polymer will behave and release drug payloads in vivo. Dynamic Light Scattering was used to monitor change in nanoparticle size, indicative of degradation. The release of a fluorescent dye, coupled to PGA, upon incubation with enzymes was measured over a 96 h period as a model of drug release from polymer drug conjugates. The changes to the chemical structure and molecular weight of PGA following enzyme exposure were characterised using FTIR, NMR and GPC. These techniques provided evidence of the biodegradability of PGA, its susceptibility to degradation by a range of enzymes commonly found in the human body and the polymer's potential as a drug delivery platform.

Authors+Show Affiliations

School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.Department of Medicine, University College London, London WC1E 6JF, United Kingdom.Drug Product Design, Pfizer Ltd, Sandwich CT13 9ND, United Kingdom.Drug Product Design, Pfizer Ltd, Sandwich CT13 9ND, United Kingdom.School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom. Electronic address: martin.garnett@nottingham.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31319123

Citation

Swainson, Sadie M E., et al. "Exploring the Enzymatic Degradation of Poly(glycerol Adipate)." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 142, 2019, pp. 377-386.
Swainson SME, Taresco V, Pearce AK, et al. Exploring the enzymatic degradation of poly(glycerol adipate). Eur J Pharm Biopharm. 2019;142:377-386.
Swainson, S. M. E., Taresco, V., Pearce, A. K., Clapp, L. H., Ager, B., McAllister, M., Bosquillon, C., & Garnett, M. C. (2019). Exploring the enzymatic degradation of poly(glycerol adipate). European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 142, 377-386. https://doi.org/10.1016/j.ejpb.2019.07.015
Swainson SME, et al. Exploring the Enzymatic Degradation of Poly(glycerol Adipate). Eur J Pharm Biopharm. 2019;142:377-386. PubMed PMID: 31319123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring the enzymatic degradation of poly(glycerol adipate). AU - Swainson,Sadie M E, AU - Taresco,Vincenzo, AU - Pearce,Amanda K, AU - Clapp,Lucie H, AU - Ager,Barry, AU - McAllister,Mark, AU - Bosquillon,Cynthia, AU - Garnett,Martin C, Y1 - 2019/07/15/ PY - 2019/05/24/received PY - 2019/06/24/revised PY - 2019/07/14/accepted PY - 2019/7/19/pubmed PY - 2019/11/26/medline PY - 2019/7/19/entrez KW - Biodegradable KW - Breakdown KW - Enzymatic degradation KW - Poly(glycerol adipate) KW - Polyester KW - Polymer modification SP - 377 EP - 386 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 142 N2 - Poly(glycerol adipate) (PGA) is a biodegradable, biocompatible, polymer with a great deal of potential in the field of drug delivery. Active drug molecules can be conjugated to the polymer backbone or encapsulated in self-assembled nanoparticles for targeted and systemic delivery. Here, a range of techniques have been used to characterise the enzymatic degradation of PGA extensively for the first time and to provide an indication of the way the polymer will behave and release drug payloads in vivo. Dynamic Light Scattering was used to monitor change in nanoparticle size, indicative of degradation. The release of a fluorescent dye, coupled to PGA, upon incubation with enzymes was measured over a 96 h period as a model of drug release from polymer drug conjugates. The changes to the chemical structure and molecular weight of PGA following enzyme exposure were characterised using FTIR, NMR and GPC. These techniques provided evidence of the biodegradability of PGA, its susceptibility to degradation by a range of enzymes commonly found in the human body and the polymer's potential as a drug delivery platform. SN - 1873-3441 UR - https://www.unboundmedicine.com/medline/citation/31319123/Exploring_the_enzymatic_degradation_of_poly_glycerol_adipate__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(19)30604-6 DB - PRIME DP - Unbound Medicine ER -