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Treponema pallidum enhances human monocyte migration and invasion by dysregulating the MMP/TIMP balance.
Int Immunopharmacol 2019; 75:105744II

Abstract

Although the infiltration of monocytes into local lesions is an obvious pathological manifestation in the pathogenesis of syphilis, little is known about the role of metalloproteinase (MMP)/tissue inhibitor of metalloproteinases (TIMP) imbalance in the migration/invasion of THP-1 cells induced by Treponema pallidum (T. pallidum). The influence of T. pallidum on the invasion and migration of THP-1 cells was evaluated. Changes in the MMP/TIMP balance and the mechanisms underlying the involvement of the MAPK and NF-κB signaling pathways in this process were explored. T. pallidum induced the migration/invasion of THP-1 cells and the mRNA and protein expression of MMP-1, MMP-9 and TIMP-1. The mRNA expression of TIMP-2 was reduced, and the protein expression of TIMP-2 was not changed. The MMP-1/TIMP-1, MMP-1/TIMP-2, MMP-9/TIMP-1 and MMP-9/TIMP-2 ratios were increased. Inhibition of JNK, MEK/ERK, p38 MAPK and NF-κB significantly decreased the MMP/TIMP ratio and ultimately suppressed the migration/invasion of THP-1 cells. These findings revealed that MMP/TIMP imbalances induced by T. pallidum enhanced THP-1 cell migration and invasion via MAPK and NF-κB signaling pathway activation, which revealed a novel step in syphilis pathophysiology.

Authors+Show Affiliations

Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China. Electronic address: liulili@xmu.edu.cn.Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China. Electronic address: yangtianci@xmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31319358

Citation

Lin, Shu-Wen, et al. "Treponema Pallidum Enhances Human Monocyte Migration and Invasion By Dysregulating the MMP/TIMP Balance." International Immunopharmacology, vol. 75, 2019, p. 105744.
Lin SW, Gao ZX, Lin LR, et al. Treponema pallidum enhances human monocyte migration and invasion by dysregulating the MMP/TIMP balance. Int Immunopharmacol. 2019;75:105744.
Lin, S. W., Gao, Z. X., Lin, L. R., Luo, X., Liu, L. L., & Yang, T. C. (2019). Treponema pallidum enhances human monocyte migration and invasion by dysregulating the MMP/TIMP balance. International Immunopharmacology, 75, p. 105744. doi:10.1016/j.intimp.2019.105744.
Lin SW, et al. Treponema Pallidum Enhances Human Monocyte Migration and Invasion By Dysregulating the MMP/TIMP Balance. Int Immunopharmacol. 2019;75:105744. PubMed PMID: 31319358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treponema pallidum enhances human monocyte migration and invasion by dysregulating the MMP/TIMP balance. AU - Lin,Shu-Wen, AU - Gao,Zheng-Xiang, AU - Lin,Li-Rong, AU - Luo,Xi, AU - Liu,Li-Li, AU - Yang,Tian-Ci, Y1 - 2019/07/15/ PY - 2019/05/19/received PY - 2019/06/24/revised PY - 2019/07/05/accepted PY - 2019/7/19/pubmed PY - 2019/7/19/medline PY - 2019/7/19/entrez KW - Invasion KW - MMP/TIMP imbalance KW - Migration KW - Monocyte KW - Treponema pallidum SP - 105744 EP - 105744 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 75 N2 - Although the infiltration of monocytes into local lesions is an obvious pathological manifestation in the pathogenesis of syphilis, little is known about the role of metalloproteinase (MMP)/tissue inhibitor of metalloproteinases (TIMP) imbalance in the migration/invasion of THP-1 cells induced by Treponema pallidum (T. pallidum). The influence of T. pallidum on the invasion and migration of THP-1 cells was evaluated. Changes in the MMP/TIMP balance and the mechanisms underlying the involvement of the MAPK and NF-κB signaling pathways in this process were explored. T. pallidum induced the migration/invasion of THP-1 cells and the mRNA and protein expression of MMP-1, MMP-9 and TIMP-1. The mRNA expression of TIMP-2 was reduced, and the protein expression of TIMP-2 was not changed. The MMP-1/TIMP-1, MMP-1/TIMP-2, MMP-9/TIMP-1 and MMP-9/TIMP-2 ratios were increased. Inhibition of JNK, MEK/ERK, p38 MAPK and NF-κB significantly decreased the MMP/TIMP ratio and ultimately suppressed the migration/invasion of THP-1 cells. These findings revealed that MMP/TIMP imbalances induced by T. pallidum enhanced THP-1 cell migration and invasion via MAPK and NF-κB signaling pathway activation, which revealed a novel step in syphilis pathophysiology. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/31319358/Treponema_pallidum_enhances_human_monocyte_migration_and_invasion_by_dysregulating_the_MMP/TIMP_balance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(19)31064-1 DB - PRIME DP - Unbound Medicine ER -