Tags

Type your tag names separated by a space and hit enter

Differences in protein profiles between Malassezia pachydermatis strains obtained from healthy and infected dogs.
Mycologia. 2019 Jul-Aug; 111(4):624-631.M

Abstract

Malassezia pachydermatis causes infections of the skin and mucous membranes, especially in individuals with metabolic, hormonal, and immunological disorders. The search for M. pachydermatis properties that differentiate isolates from healthy and infected animals may result in the identification of typically commensal and potentially pathogenic strains within the entire species. We aimed to determine and compare protein profiles of M. pachydermatis strains isolated from 30 dogs with clinical symptoms of otitis externa and 34 dogs without symptoms of any disease. Two-dimensional gel electrophoresis was applied, and proteins distinguishing the two groups of strains were identified by liquid chromatography coupled with tandem mass spectrometry. Significant differences were found between potentially pathogenic and commensal isolates. The most significant finding was the presence of nicotinamide adenine dinucleotide phosphate (NADP)-dependent mannitol dehydrogenase and ketol-acid reductoisomerase among M. pachydermatis strains obtained from dogs with otitis externa. Nevertheless, it is not clear whether they are associated directly with the pathogenicity or they play the role of fungal allergen. On the basis of these findings, we can conclude that there may be two distinct groups of M. pachydermatis strains-one typically commensal and the other with properties that enhance the infection process. These results may be used for more precise diagnosis and identification of potentially pathogenic strains in the future.

Authors+Show Affiliations

a Department of Cytobiochemistry, Institute of Biology, Faculty of Biology and Chemistry, University of Bialystok , Ciolkowskiego 1J, 15-245 Bialystok , Poland.b Department of Comparative Biochemistry and Bioanalytics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Gronostajowa 7, 30-387 Cracow , Poland.c Department of Microbiology, Institute of Biology, Faculty of Biology and Chemistry, University of Bialystok , Ciolkowskiego 1J, 15-245 Bialystok , Poland.a Department of Cytobiochemistry, Institute of Biology, Faculty of Biology and Chemistry, University of Bialystok , Ciolkowskiego 1J, 15-245 Bialystok , Poland.a Department of Cytobiochemistry, Institute of Biology, Faculty of Biology and Chemistry, University of Bialystok , Ciolkowskiego 1J, 15-245 Bialystok , Poland.a Department of Cytobiochemistry, Institute of Biology, Faculty of Biology and Chemistry, University of Bialystok , Ciolkowskiego 1J, 15-245 Bialystok , Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31322986

Citation

Czyżewska, Urszula, et al. "Differences in Protein Profiles Between Malassezia Pachydermatis Strains Obtained From Healthy and Infected Dogs." Mycologia, vol. 111, no. 4, 2019, pp. 624-631.
Czyżewska U, Karkowska-Kuleta J, Bartoszewicz M, et al. Differences in protein profiles between Malassezia pachydermatis strains obtained from healthy and infected dogs. Mycologia. 2019;111(4):624-631.
Czyżewska, U., Karkowska-Kuleta, J., Bartoszewicz, M., Siemieniuk, M., Zambrzycka, A., & Tylicki, A. (2019). Differences in protein profiles between Malassezia pachydermatis strains obtained from healthy and infected dogs. Mycologia, 111(4), 624-631. https://doi.org/10.1080/00275514.2019.1630244
Czyżewska U, et al. Differences in Protein Profiles Between Malassezia Pachydermatis Strains Obtained From Healthy and Infected Dogs. Mycologia. 2019;111(4):624-631. PubMed PMID: 31322986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in protein profiles between Malassezia pachydermatis strains obtained from healthy and infected dogs. AU - Czyżewska,Urszula, AU - Karkowska-Kuleta,Justyna, AU - Bartoszewicz,Marek, AU - Siemieniuk,Magdalena, AU - Zambrzycka,Aneta, AU - Tylicki,Adam, Y1 - 2019/07/19/ PY - 2019/7/20/pubmed PY - 2019/7/20/medline PY - 2019/7/20/entrez KW - Characteristic proteins KW - otitis externa KW - two-dimensional electrophoresis KW - yeasts SP - 624 EP - 631 JF - Mycologia JO - Mycologia VL - 111 IS - 4 N2 - Malassezia pachydermatis causes infections of the skin and mucous membranes, especially in individuals with metabolic, hormonal, and immunological disorders. The search for M. pachydermatis properties that differentiate isolates from healthy and infected animals may result in the identification of typically commensal and potentially pathogenic strains within the entire species. We aimed to determine and compare protein profiles of M. pachydermatis strains isolated from 30 dogs with clinical symptoms of otitis externa and 34 dogs without symptoms of any disease. Two-dimensional gel electrophoresis was applied, and proteins distinguishing the two groups of strains were identified by liquid chromatography coupled with tandem mass spectrometry. Significant differences were found between potentially pathogenic and commensal isolates. The most significant finding was the presence of nicotinamide adenine dinucleotide phosphate (NADP)-dependent mannitol dehydrogenase and ketol-acid reductoisomerase among M. pachydermatis strains obtained from dogs with otitis externa. Nevertheless, it is not clear whether they are associated directly with the pathogenicity or they play the role of fungal allergen. On the basis of these findings, we can conclude that there may be two distinct groups of M. pachydermatis strains-one typically commensal and the other with properties that enhance the infection process. These results may be used for more precise diagnosis and identification of potentially pathogenic strains in the future. SN - 1557-2536 UR - https://www.unboundmedicine.com/medline/citation/31322986/Differences_in_protein_profiles_between_Malassezia_pachydermatis_strains_obtained_from_healthy_and_infected_dogs L2 - http://www.tandfonline.com/doi/full/10.1080/00275514.2019.1630244 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.