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Geographic Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) genotype diversity in India.
Acta Trop 2019; :105095AT

Abstract

Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) is sarcoplasmic reticulum membrane bound transporter to regulate cytosol Ca2+ ions. Ca2+ act as secondary messenger and play important role in differentiation of parasite during its life cycle. Present study is epidemiological surveillance of PfSERCA (Pf3D7_0106300) gene fragment harboring 263, 402, 431 codon to look for its single nucleotide polymorphism which is well documented to be associated with Artemisinin tolerance. Filter paper with finger pricked blood samples for Plasmodium falciparum infected uncomplicated malaria patients were obtained for region as diverse as down the longitude from east to west of India i.e. Mizoram, Tripura, Meghalaya, Jharkhand, Odhisa. There observed no mutation for codon 263 at all study sites. Mizoram showed highest PfSERCA diversity with well known SNPs of L402 V, E431 K, A438 V and novel mutations as well i.e. A338 V, S357Y, S379Y. Tripura reported highest proportion of Plasmodium isolates (18.5%) with E431 K single nucleotide polymorphism. Moving towards the west i.e. Meghalaya, Jharkhand, Odhisa showed no occurrence of most prevalent PfSERCA 431, 402 polymorphism worldwide but some novel mutations and its haplotypes. In present study, significantly increased proportion of novel PfSERCA polymorphism among children suggests the susceptibility of these Plasmodium falciparum strains to acquired immunity. Mizoram, sharing open international border with south east asia, demonstrated highest PfSERCA diversity. Spatial PfSERCA diversity from far north east India to moving towards west implies its association with antimalarial susceptibility.

Authors+Show Affiliations

National Institute of Malaria Research (Indian Council of Medical Research), Sector - 8, Dwarka, New Delhi 110 077, India. Electronic address: shelly.sahni@rediffmail.com.National Institute of Malaria Research (Indian Council of Medical Research), Sector - 8, Dwarka, New Delhi 110 077, India.National Institute of Malaria Research (Indian Council of Medical Research), Sector - 8, Dwarka, New Delhi 110 077, India.National Institute of Malaria Research (Indian Council of Medical Research), Sector - 8, Dwarka, New Delhi 110 077, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31323193

Citation

Goomber, Shelly, et al. "Geographic Plasmodium Falciparum Sarcoplasmic Reticulum Ca2+ ATPase (PfSERCA) Genotype Diversity in India." Acta Tropica, 2019, p. 105095.
Goomber S, Mishra N, Anvikar A, et al. Geographic Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) genotype diversity in India. Acta Trop. 2019.
Goomber, S., Mishra, N., Anvikar, A., & Valecha, N. (2019). Geographic Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) genotype diversity in India. Acta Tropica, p. 105095. doi:10.1016/j.actatropica.2019.105095.
Goomber S, et al. Geographic Plasmodium Falciparum Sarcoplasmic Reticulum Ca2+ ATPase (PfSERCA) Genotype Diversity in India. Acta Trop. 2019 Jul 16;105095. PubMed PMID: 31323193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Geographic Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) genotype diversity in India. AU - Goomber,Shelly, AU - Mishra,Neelima, AU - Anvikar,Anup, AU - Valecha,Neena, Y1 - 2019/07/16/ PY - 2018/03/30/received PY - 2018/11/18/revised PY - 2019/07/15/accepted PY - 2019/7/20/entrez PY - 2019/7/20/pubmed PY - 2019/7/20/medline KW - Artemisinin KW - Plasmodium falciparum KW - Southeast Asia KW - single nucleotide polymorphism SP - 105095 EP - 105095 JF - Acta tropica JO - Acta Trop. N2 - Plasmodium falciparum sarcoplasmic reticulum Ca2+ ATPase (PfSERCA) is sarcoplasmic reticulum membrane bound transporter to regulate cytosol Ca2+ ions. Ca2+ act as secondary messenger and play important role in differentiation of parasite during its life cycle. Present study is epidemiological surveillance of PfSERCA (Pf3D7_0106300) gene fragment harboring 263, 402, 431 codon to look for its single nucleotide polymorphism which is well documented to be associated with Artemisinin tolerance. Filter paper with finger pricked blood samples for Plasmodium falciparum infected uncomplicated malaria patients were obtained for region as diverse as down the longitude from east to west of India i.e. Mizoram, Tripura, Meghalaya, Jharkhand, Odhisa. There observed no mutation for codon 263 at all study sites. Mizoram showed highest PfSERCA diversity with well known SNPs of L402 V, E431 K, A438 V and novel mutations as well i.e. A338 V, S357Y, S379Y. Tripura reported highest proportion of Plasmodium isolates (18.5%) with E431 K single nucleotide polymorphism. Moving towards the west i.e. Meghalaya, Jharkhand, Odhisa showed no occurrence of most prevalent PfSERCA 431, 402 polymorphism worldwide but some novel mutations and its haplotypes. In present study, significantly increased proportion of novel PfSERCA polymorphism among children suggests the susceptibility of these Plasmodium falciparum strains to acquired immunity. Mizoram, sharing open international border with south east asia, demonstrated highest PfSERCA diversity. Spatial PfSERCA diversity from far north east India to moving towards west implies its association with antimalarial susceptibility. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/31323193/Geographic_Plasmodium_falciparum_sarcoplasmic_reticulum_Ca2+_ATPase_(PfSERCA)_genotype_diversity_in_India L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(18)30405-4 DB - PRIME DP - Unbound Medicine ER -