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Effects of 2,4-D-based herbicide (DMA® 806) on sensitivity, respiration rates, energy reserves and behavior of tadpoles.
Ecotoxicol Environ Saf 2019; 182:109446EE

Abstract

Increased use of sugarcane pesticides and their destination to non-target environments in Brazil has generated concerns related to the conservation of more vulnerable groups, such as amphibians. Besides the high skin permeability, tadpoles are constantly restricted to small and ephemeral ponds, where exposure to high concentrations of pesticides in agricultural areas is inevitable. This study evaluated chronic effects caused by sub-lethal concentrations of 2,4-dichlorophenoxyacetic acid herbicide on energy storage, development, respiration rates, swimming performance and avoidance behavior of bullfrog tadpoles (Lithobates catesbeianus). Firstly, we conducted acute toxicity test (96 h) to estipulate sub-lethal concentrations of 2,4-D and evaluate the sensitivity of three tadpoles' species to this herbicide. Results showed that Leptodactylus fuscus presented the lowest LC50 96 h, 28.81 mg/L, followed by Physalaemus nattereri (143.08 mg/L) and L. catesbeianus (574.52 mg/L). Chronic exposure to 2,4-D (125, 250 and 500 μg/L) delayed metamorphosis and inhibited the growth of tadpoles at concentrations of 125 μg/L. Effects on biochemical reserves showed that 2,4-D increased total hepatic lipids in tadpoles, although some individual lipid classes (e.g. free fatty acids and triglycerides) were reduced. Protein and carbohydrates contents were also impaired by 2,4-D, suggesting a disruption on energy metabolism of amphibians by the herbicide. In addition to biochemical changes, respiration rates and swimming speed were also decreased after chronic exposure to 2,4-D, and these responses appeared to be correlated with the changes detected in the basic energy content. Avoidance test indicated that tadpoles of L. catesbeinus avoided the presence of 2,4-D, however they were unable to detect increasing gradients of the contaminant. Our data showed that chronic exposure to 2,4-D impaired biochemical, physiological and behavioral aspects of tadpoles, which may compromise their health and make them more vulnerable to environmental stressors in natural systems.

Authors+Show Affiliations

NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, SP, Brazil. Electronic address: julianefbio@gmail.com.NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, SP, Brazil.Analytical Chemistry Department, Institute of Chemistry, University of Campinas, Campinas, SP, Brazil.Department of Hydrobiology, Federal University of São Carlos, São Carlos, SP, Brazil.Department of Hydrobiology, Federal University of São Carlos, São Carlos, SP, Brazil.Analytical Chemistry Department, Institute of Chemistry, University of Campinas, Campinas, SP, Brazil.EACH, USP - School of Arts, Sciences and Humanities, São Paulo, SP, Brazil.NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, SP, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31323523

Citation

Freitas, Juliane Silberschmidt, et al. "Effects of 2,4-D-based Herbicide (DMA® 806) On Sensitivity, Respiration Rates, Energy Reserves and Behavior of Tadpoles." Ecotoxicology and Environmental Safety, vol. 182, 2019, p. 109446.
Freitas JS, Girotto L, Goulart BV, et al. Effects of 2,4-D-based herbicide (DMA® 806) on sensitivity, respiration rates, energy reserves and behavior of tadpoles. Ecotoxicol Environ Saf. 2019;182:109446.
Freitas, J. S., Girotto, L., Goulart, B. V., Alho, L. O. G., Gebara, R. C., Montagner, C. C., ... Espíndola, E. L. G. (2019). Effects of 2,4-D-based herbicide (DMA® 806) on sensitivity, respiration rates, energy reserves and behavior of tadpoles. Ecotoxicology and Environmental Safety, 182, p. 109446. doi:10.1016/j.ecoenv.2019.109446.
Freitas JS, et al. Effects of 2,4-D-based Herbicide (DMA® 806) On Sensitivity, Respiration Rates, Energy Reserves and Behavior of Tadpoles. Ecotoxicol Environ Saf. 2019 Oct 30;182:109446. PubMed PMID: 31323523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of 2,4-D-based herbicide (DMA® 806) on sensitivity, respiration rates, energy reserves and behavior of tadpoles. AU - Freitas,Juliane Silberschmidt, AU - Girotto,Laís, AU - Goulart,Bianca Veloso, AU - Alho,Lays de Oliveira Gonçalves, AU - Gebara,Renan Castelhano, AU - Montagner,Cassiana Carolina, AU - Schiesari,Luis, AU - Espíndola,Evaldo Luiz Gaeta, Y1 - 2019/07/16/ PY - 2019/02/19/received PY - 2019/06/22/revised PY - 2019/07/11/accepted PY - 2019/7/20/pubmed PY - 2019/7/20/medline PY - 2019/7/20/entrez KW - Avoidance KW - Behavior KW - Biochemical reserves KW - Sugarcane KW - Tadpoles SP - 109446 EP - 109446 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 182 N2 - Increased use of sugarcane pesticides and their destination to non-target environments in Brazil has generated concerns related to the conservation of more vulnerable groups, such as amphibians. Besides the high skin permeability, tadpoles are constantly restricted to small and ephemeral ponds, where exposure to high concentrations of pesticides in agricultural areas is inevitable. This study evaluated chronic effects caused by sub-lethal concentrations of 2,4-dichlorophenoxyacetic acid herbicide on energy storage, development, respiration rates, swimming performance and avoidance behavior of bullfrog tadpoles (Lithobates catesbeianus). Firstly, we conducted acute toxicity test (96 h) to estipulate sub-lethal concentrations of 2,4-D and evaluate the sensitivity of three tadpoles' species to this herbicide. Results showed that Leptodactylus fuscus presented the lowest LC50 96 h, 28.81 mg/L, followed by Physalaemus nattereri (143.08 mg/L) and L. catesbeianus (574.52 mg/L). Chronic exposure to 2,4-D (125, 250 and 500 μg/L) delayed metamorphosis and inhibited the growth of tadpoles at concentrations of 125 μg/L. Effects on biochemical reserves showed that 2,4-D increased total hepatic lipids in tadpoles, although some individual lipid classes (e.g. free fatty acids and triglycerides) were reduced. Protein and carbohydrates contents were also impaired by 2,4-D, suggesting a disruption on energy metabolism of amphibians by the herbicide. In addition to biochemical changes, respiration rates and swimming speed were also decreased after chronic exposure to 2,4-D, and these responses appeared to be correlated with the changes detected in the basic energy content. Avoidance test indicated that tadpoles of L. catesbeinus avoided the presence of 2,4-D, however they were unable to detect increasing gradients of the contaminant. Our data showed that chronic exposure to 2,4-D impaired biochemical, physiological and behavioral aspects of tadpoles, which may compromise their health and make them more vulnerable to environmental stressors in natural systems. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/31323523/Effects_of_2,4-D-based_herbicide_(DMA®_806)_on_sensitivity,_respiration_rates,_energy_reserves_and_behavior_of_tadpoles L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(19)30777-8 DB - PRIME DP - Unbound Medicine ER -