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Video-tracking and high-speed bright field microscopy allow the determination of swimming and cardiac effects of D. magna exposed to local anaesthetics.
Sci Total Environ 2019; 691:278-283ST

Abstract

Local anaesthetics are among the most used drugs in clinical practice, but once they are released to the environment, the effects on the aquatic fauna remain uncertain. This study evaluates, for the first time, the impact of tetracaine, lidocaine and bupivacaine on the survival rate and physiological effects of cladocera Daphnia magna. Video-tracking and image processing allowed us to obtain changes in behaviour parameters like swimming average velocity and mean square displacement. We found that tetracaine shows the most severe effect. A high-speed microscopy system was also used to determine the response of D. magna heart to these drugs. Our results show that tetracaine presents dose-dependent area reduction during all cardiac cycle, while bupivacaine and lidocaine did not present significative effects on heart size. The tested drugs, at environmental high concentrations, altered behaviour, heart function and survival of D. magna.

Authors+Show Affiliations

Unidad Monterrey, Centro de Investigación y de Estudios Avanzados, Vía del Conocimiento 201, PIIT, Autopista al Aeropuerto, Km. 9.5, Apodaca, Nuevo León, Mexico.Unidad Monterrey, Centro de Investigación y de Estudios Avanzados, Vía del Conocimiento 201, PIIT, Autopista al Aeropuerto, Km. 9.5, Apodaca, Nuevo León, Mexico.Unidad Monterrey, Centro de Investigación y de Estudios Avanzados, Vía del Conocimiento 201, PIIT, Autopista al Aeropuerto, Km. 9.5, Apodaca, Nuevo León, Mexico. Electronic address: cruiz@cinvestav.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31323573

Citation

Robledo-Sánchez, K Carlo Martín, et al. "Video-tracking and High-speed Bright Field Microscopy Allow the Determination of Swimming and Cardiac Effects of D. Magna Exposed to Local Anaesthetics." The Science of the Total Environment, vol. 691, 2019, pp. 278-283.
Robledo-Sánchez KCM, Guevara-Pantoja FJ, Ruiz-Suárez JC. Video-tracking and high-speed bright field microscopy allow the determination of swimming and cardiac effects of D. magna exposed to local anaesthetics. Sci Total Environ. 2019;691:278-283.
Robledo-Sánchez, K. C. M., Guevara-Pantoja, F. J., & Ruiz-Suárez, J. C. (2019). Video-tracking and high-speed bright field microscopy allow the determination of swimming and cardiac effects of D. magna exposed to local anaesthetics. The Science of the Total Environment, 691, pp. 278-283. doi:10.1016/j.scitotenv.2019.06.538.
Robledo-Sánchez KCM, Guevara-Pantoja FJ, Ruiz-Suárez JC. Video-tracking and High-speed Bright Field Microscopy Allow the Determination of Swimming and Cardiac Effects of D. Magna Exposed to Local Anaesthetics. Sci Total Environ. 2019 Jul 2;691:278-283. PubMed PMID: 31323573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Video-tracking and high-speed bright field microscopy allow the determination of swimming and cardiac effects of D. magna exposed to local anaesthetics. AU - Robledo-Sánchez,K Carlo Martín, AU - Guevara-Pantoja,F J, AU - Ruiz-Suárez,J C, Y1 - 2019/07/02/ PY - 2019/05/03/received PY - 2019/06/27/revised PY - 2019/06/30/accepted PY - 2019/7/20/pubmed PY - 2019/7/20/medline PY - 2019/7/20/entrez KW - Bupivacaine KW - Daphnia magna KW - Ecotoxicity KW - Environmental risk KW - Heart rate KW - Lethal concentration KW - Lidocaine KW - Local anaesthetics KW - Swimming performance KW - Tetracaine SP - 278 EP - 283 JF - The Science of the total environment JO - Sci. Total Environ. VL - 691 N2 - Local anaesthetics are among the most used drugs in clinical practice, but once they are released to the environment, the effects on the aquatic fauna remain uncertain. This study evaluates, for the first time, the impact of tetracaine, lidocaine and bupivacaine on the survival rate and physiological effects of cladocera Daphnia magna. Video-tracking and image processing allowed us to obtain changes in behaviour parameters like swimming average velocity and mean square displacement. We found that tetracaine shows the most severe effect. A high-speed microscopy system was also used to determine the response of D. magna heart to these drugs. Our results show that tetracaine presents dose-dependent area reduction during all cardiac cycle, while bupivacaine and lidocaine did not present significative effects on heart size. The tested drugs, at environmental high concentrations, altered behaviour, heart function and survival of D. magna. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/31323573/Video-tracking_and_high-speed_bright_field_microscopy_allow_the_determination_of_swimming_and_cardiac_effects_of_D._magna_exposed_to_local_anaesthetics L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(19)33097-9 DB - PRIME DP - Unbound Medicine ER -