Tags

Type your tag names separated by a space and hit enter

Propionate alleviates high-fat diet-induced lipid dysmetabolism by modulating gut microbiota in mice.
J Appl Microbiol. 2019 Nov; 127(5):1546-1555.JA

Abstract

AIMS

The aims were to examine whether oral sodium propionate supplementation regulate lipid metabolism through modulating gut microbiota.

METHODS AND RESULTS

ICR male mice (26·98 ± 0·30 g) were randomly assigned to three groups (n = 10) and fed control diet (Con), high-fat diet (HFD) and HFD plus propionate (Pro) respectively. In this study, we found that HFD increased the weight of final body, inguinal white adipose tissues (iWAT), epididymal white adipose tissue (eWAT) and perirenal white adipose tissue (pWAT), as well as the adipocyte mean area of iWAT and eWAT in mice (P < 0·05), whereas sodium propionate treatment reduced the weight of iWAT and pWAT as well as adipocyte mean area of iWAT in mice fed a HFD (P < 0·05). Moreover, in the iWAT, the mRNA expression of lipogenesis genes, including peroxisome proliferator activated receptor γ, acetyl-CoA carboxylase and carnitine palmitoyl transferase-1β, was upregulated by HFD challenge (P < 0·05), and the elevation of these genes was nearly reversed to the level of control diet-fed mice by sodium propionate treatment. Meanwhile, sodium propionate treatment increased the hormone-sensitive lipase mRNA expression in the iWAT of HFD-fed mice (P < 0·05). High-throughput pyrosequencing of the 16S rRNA demonstrated that sodium propionate treatment significantly recovered the gut microbiota dysbiosis in HFD-fed mice, including the richness and diversity of microbiota and the ratio of Firmicutes to Bacteroidetes. Furthermore, the HFD-induced reductions in colonic levels of butyrate and valerate were reversed by sodium propionate treatment, which also normalized the serum LPS level seen in HFD-fed mice to the levels of the control diet-fed mice.

CONCLUSIONS

Collectively, these results indicated that sodium propionate treatment could improve lipid metabolism in HFD-fed mice, and the potential mechanisms might be via regulating gut microbiota.

SIGNIFICANCE AND IMPACT OF THE STUDY

We demonstrated for the first time that oral sodium propionate significantly improved HFD-induced dysbiosis of gut microbiota, indicating that the mitigative effect of propionate for HFD-induced lipid dysmetabolism might be mediated by gut microbiota in mice.

Authors+Show Affiliations

Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China. Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China. Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China. Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, China. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China. Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, China.Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31325215

Citation

Song, B, et al. "Propionate Alleviates High-fat Diet-induced Lipid Dysmetabolism By Modulating Gut Microbiota in Mice." Journal of Applied Microbiology, vol. 127, no. 5, 2019, pp. 1546-1555.
Song B, Zhong YZ, Zheng CB, et al. Propionate alleviates high-fat diet-induced lipid dysmetabolism by modulating gut microbiota in mice. J Appl Microbiol. 2019;127(5):1546-1555.
Song, B., Zhong, Y. Z., Zheng, C. B., Li, F. N., Duan, Y. H., & Deng, J. P. (2019). Propionate alleviates high-fat diet-induced lipid dysmetabolism by modulating gut microbiota in mice. Journal of Applied Microbiology, 127(5), 1546-1555. https://doi.org/10.1111/jam.14389
Song B, et al. Propionate Alleviates High-fat Diet-induced Lipid Dysmetabolism By Modulating Gut Microbiota in Mice. J Appl Microbiol. 2019;127(5):1546-1555. PubMed PMID: 31325215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Propionate alleviates high-fat diet-induced lipid dysmetabolism by modulating gut microbiota in mice. AU - Song,B, AU - Zhong,Y Z, AU - Zheng,C B, AU - Li,F N, AU - Duan,Y H, AU - Deng,J P, Y1 - 2019/08/15/ PY - 2019/06/05/received PY - 2019/07/04/revised PY - 2019/07/06/accepted PY - 2019/7/22/pubmed PY - 2019/12/18/medline PY - 2019/7/21/entrez KW - gut microbiota KW - high-fat diet KW - lipid metabolism KW - oral administration KW - sodium propionate SP - 1546 EP - 1555 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 127 IS - 5 N2 - AIMS: The aims were to examine whether oral sodium propionate supplementation regulate lipid metabolism through modulating gut microbiota. METHODS AND RESULTS: ICR male mice (26·98 ± 0·30 g) were randomly assigned to three groups (n = 10) and fed control diet (Con), high-fat diet (HFD) and HFD plus propionate (Pro) respectively. In this study, we found that HFD increased the weight of final body, inguinal white adipose tissues (iWAT), epididymal white adipose tissue (eWAT) and perirenal white adipose tissue (pWAT), as well as the adipocyte mean area of iWAT and eWAT in mice (P < 0·05), whereas sodium propionate treatment reduced the weight of iWAT and pWAT as well as adipocyte mean area of iWAT in mice fed a HFD (P < 0·05). Moreover, in the iWAT, the mRNA expression of lipogenesis genes, including peroxisome proliferator activated receptor γ, acetyl-CoA carboxylase and carnitine palmitoyl transferase-1β, was upregulated by HFD challenge (P < 0·05), and the elevation of these genes was nearly reversed to the level of control diet-fed mice by sodium propionate treatment. Meanwhile, sodium propionate treatment increased the hormone-sensitive lipase mRNA expression in the iWAT of HFD-fed mice (P < 0·05). High-throughput pyrosequencing of the 16S rRNA demonstrated that sodium propionate treatment significantly recovered the gut microbiota dysbiosis in HFD-fed mice, including the richness and diversity of microbiota and the ratio of Firmicutes to Bacteroidetes. Furthermore, the HFD-induced reductions in colonic levels of butyrate and valerate were reversed by sodium propionate treatment, which also normalized the serum LPS level seen in HFD-fed mice to the levels of the control diet-fed mice. CONCLUSIONS: Collectively, these results indicated that sodium propionate treatment could improve lipid metabolism in HFD-fed mice, and the potential mechanisms might be via regulating gut microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated for the first time that oral sodium propionate significantly improved HFD-induced dysbiosis of gut microbiota, indicating that the mitigative effect of propionate for HFD-induced lipid dysmetabolism might be mediated by gut microbiota in mice. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/31325215/Propionate_alleviates_high_fat_diet_induced_lipid_dysmetabolism_by_modulating_gut_microbiota_in_mice_ L2 - https://doi.org/10.1111/jam.14389 DB - PRIME DP - Unbound Medicine ER -