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MicroRNA-152-3p protects neurons from oxygen-glucose-deprivation/reoxygenation-induced injury through upregulation of Nrf2/ARE antioxidant signaling by targeting PSD-93.
Biochem Biophys Res Commun. 2019 09 10; 517(1):69-76.BB

Abstract

MicroRNAs (miRNAs) have emerged as critical regulators of ischemic stroke, a condition that affects neuronal survival. However, the precise role of miRNAs in regulating neuronal injury during ischemic stroke remains largely unknown. In this study, we investigated the potential role of miR-152-3p in regulating oxygen-glucose-deprivation/reoxygenation (OGD/R)-induced neuronal injury in vitro. We found that OGD/R-exposed neurons expressed less miR-152-3p. Functional analysis revealed that miR-152-3p overexpression increased the viability and reduced the apoptosis and reactive oxygen species (ROS) production of OGD/R-exposed neurons. By contrast, miR-152-3p inhibition exacerbated OGD/R-induced injury. Notably, we identified postsynaptic density protein-93 (PSD-93), an important regulator of neuroprotection during ischemic stroke, as a miR-152-3p target gene. PSD-93 inhibition by small interfering RNA (siRNA) or miR-152-3p reinforced the activation of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) antioxidant signaling in OGD/R-exposed neurons. However, PSD-93 overexpression or Nrf2 silencing partially reversed miR-152-3p-mediated neuroprotection in OGD/R-exposed neurons. Overall, these results demonstrated that miR-152-3p protected neurons from OGD/R-induced apoptosis and ROS production by reinforcing Nrf2/ARE antioxidant signaling through targeting and inhibiting PSD-93, findings that suggest miR-152-3p is a potential target for neuroprotection during ischemic stroke.

Authors+Show Affiliations

Department of Neurology, Xi'an No.4 Hospital, Xi'an, 710004, China.Department of Clinical Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, China.Department of Neurology, Xi'an No.4 Hospital, Xi'an, 710004, China. Electronic address: qj_xp@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31326116

Citation

Zhang, Aixiang, et al. "MicroRNA-152-3p Protects Neurons From Oxygen-glucose-deprivation/reoxygenation-induced Injury Through Upregulation of Nrf2/ARE Antioxidant Signaling By Targeting PSD-93." Biochemical and Biophysical Research Communications, vol. 517, no. 1, 2019, pp. 69-76.
Zhang A, Qian Y, Qian J. MicroRNA-152-3p protects neurons from oxygen-glucose-deprivation/reoxygenation-induced injury through upregulation of Nrf2/ARE antioxidant signaling by targeting PSD-93. Biochem Biophys Res Commun. 2019;517(1):69-76.
Zhang, A., Qian, Y., & Qian, J. (2019). MicroRNA-152-3p protects neurons from oxygen-glucose-deprivation/reoxygenation-induced injury through upregulation of Nrf2/ARE antioxidant signaling by targeting PSD-93. Biochemical and Biophysical Research Communications, 517(1), 69-76. https://doi.org/10.1016/j.bbrc.2019.07.012
Zhang A, Qian Y, Qian J. MicroRNA-152-3p Protects Neurons From Oxygen-glucose-deprivation/reoxygenation-induced Injury Through Upregulation of Nrf2/ARE Antioxidant Signaling By Targeting PSD-93. Biochem Biophys Res Commun. 2019 09 10;517(1):69-76. PubMed PMID: 31326116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-152-3p protects neurons from oxygen-glucose-deprivation/reoxygenation-induced injury through upregulation of Nrf2/ARE antioxidant signaling by targeting PSD-93. AU - Zhang,Aixiang, AU - Qian,Yuanjie, AU - Qian,Jian, Y1 - 2019/07/17/ PY - 2019/06/25/received PY - 2019/07/04/accepted PY - 2019/7/22/pubmed PY - 2020/5/27/medline PY - 2019/7/22/entrez KW - Ischemic stroke KW - Nrf2 KW - Oxygen-glucose deprivation/reoxygenation KW - PSD-93 KW - miR-152-3p SP - 69 EP - 76 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 517 IS - 1 N2 - MicroRNAs (miRNAs) have emerged as critical regulators of ischemic stroke, a condition that affects neuronal survival. However, the precise role of miRNAs in regulating neuronal injury during ischemic stroke remains largely unknown. In this study, we investigated the potential role of miR-152-3p in regulating oxygen-glucose-deprivation/reoxygenation (OGD/R)-induced neuronal injury in vitro. We found that OGD/R-exposed neurons expressed less miR-152-3p. Functional analysis revealed that miR-152-3p overexpression increased the viability and reduced the apoptosis and reactive oxygen species (ROS) production of OGD/R-exposed neurons. By contrast, miR-152-3p inhibition exacerbated OGD/R-induced injury. Notably, we identified postsynaptic density protein-93 (PSD-93), an important regulator of neuroprotection during ischemic stroke, as a miR-152-3p target gene. PSD-93 inhibition by small interfering RNA (siRNA) or miR-152-3p reinforced the activation of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) antioxidant signaling in OGD/R-exposed neurons. However, PSD-93 overexpression or Nrf2 silencing partially reversed miR-152-3p-mediated neuroprotection in OGD/R-exposed neurons. Overall, these results demonstrated that miR-152-3p protected neurons from OGD/R-induced apoptosis and ROS production by reinforcing Nrf2/ARE antioxidant signaling through targeting and inhibiting PSD-93, findings that suggest miR-152-3p is a potential target for neuroprotection during ischemic stroke. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/31326116/MicroRNA_152_3p_protects_neurons_from_oxygen_glucose_deprivation/reoxygenation_induced_injury_through_upregulation_of_Nrf2/ARE_antioxidant_signaling_by_targeting_PSD_93_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(19)31341-5 DB - PRIME DP - Unbound Medicine ER -