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Is there any relationship between human sperm parameters and protamine deficiency in different groups of infertile men?
Rev Int Androl. 2019 Jul 18 [Online ahead of print]RI

Abstract

OBJECTIVE

Abnormality in Histone-Protamine replacements has been indicated to cause sperm DNA damage and infertility. The aim of the present study was to investigate the relationships between sperm parameters in oligospermia, asthenospermia, and teratospermia with protamine deficiency in infertile men.

MATERIAL AND METHOD

In this case-control study, we had three experimental groups including oligospermia (n=100), asthenospermia (n=100), and teratospermia (n=100) as well as normospermia (n=100) as controls. Sperm analyses were performed according to the recommendations of the World Health Organization (WHO, 2010) and sperm chromatin quality was assessed using Chromomycin A3 (CMA3) staining for each sample.

RESULTS

The comparison of the data between groups indicated that the percentage of spermatozoa with protamine deficiency was significantly different in patients with oligospermia, asthenospermia, and teratospermia when compared with control ones. However, there was no significant correlation between sperm nuclear protamine deficiency and their parameters of the men with teratospermia using CMA3 test. Regarding the oligospermia and asthenospermia semen samples, the findings showed the negative correlations between the sperm nuclear protamine deficiency and progressive motility as well as immobility (p<0.001).

CONCLUSION

The higher proportion of spermatozoa with abnormal chromatin packaging was observed in asthenospermic samples than those from other experimental groups as well as controls. It seems that normal morphology cannot have a valuable predictive value for good chromatin quality of spermatozoa, as much as normal motility characteristics, since samples with high mobility rates often have lower protamine deficiencies. The findings may provide a supportable promoting the future wider clinical application of chromatin/DNA integrity testing along with the semen analysis in male infertility.

Authors+Show Affiliations

Andrology Department, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Andrology Department, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Abortion Research Center, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Andrology Department, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Andrology Department, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Abortion Research Center, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Electronic address: prof_talebi@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31326281

Citation

Dehghanpour, Fatemeh, et al. "Is There Any Relationship Between Human Sperm Parameters and Protamine Deficiency in Different Groups of Infertile Men?" Revista Internacional De Andrologia, 2019.
Dehghanpour F, Fesahat F, Yazdinejad F, et al. Is there any relationship between human sperm parameters and protamine deficiency in different groups of infertile men? Rev Int Androl. 2019.
Dehghanpour, F., Fesahat, F., Yazdinejad, F., Motamedzadeh, L., & Talebi, A. R. (2019). Is there any relationship between human sperm parameters and protamine deficiency in different groups of infertile men? Revista Internacional De Andrologia. https://doi.org/10.1016/j.androl.2019.04.003
Dehghanpour F, et al. Is There Any Relationship Between Human Sperm Parameters and Protamine Deficiency in Different Groups of Infertile Men. Rev Int Androl. 2019 Jul 18; PubMed PMID: 31326281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is there any relationship between human sperm parameters and protamine deficiency in different groups of infertile men? AU - Dehghanpour,Fatemeh, AU - Fesahat,Farzaneh, AU - Yazdinejad,Fatemeh, AU - Motamedzadeh,Leila, AU - Talebi,Ali Reza, Y1 - 2019/07/18/ PY - 2018/06/18/received PY - 2019/01/19/revised PY - 2019/04/15/accepted PY - 2019/7/22/entrez PY - 2019/7/22/pubmed PY - 2019/7/22/medline KW - Astenospermia KW - Asthenospermia KW - Deficiencia de protamina KW - Esperma KW - Oligospermia KW - Protamine deficiency KW - Sperm KW - Teratozoospermia JF - Revista internacional de andrologia JO - Rev Int Androl N2 - OBJECTIVE: Abnormality in Histone-Protamine replacements has been indicated to cause sperm DNA damage and infertility. The aim of the present study was to investigate the relationships between sperm parameters in oligospermia, asthenospermia, and teratospermia with protamine deficiency in infertile men. MATERIAL AND METHOD: In this case-control study, we had three experimental groups including oligospermia (n=100), asthenospermia (n=100), and teratospermia (n=100) as well as normospermia (n=100) as controls. Sperm analyses were performed according to the recommendations of the World Health Organization (WHO, 2010) and sperm chromatin quality was assessed using Chromomycin A3 (CMA3) staining for each sample. RESULTS: The comparison of the data between groups indicated that the percentage of spermatozoa with protamine deficiency was significantly different in patients with oligospermia, asthenospermia, and teratospermia when compared with control ones. However, there was no significant correlation between sperm nuclear protamine deficiency and their parameters of the men with teratospermia using CMA3 test. Regarding the oligospermia and asthenospermia semen samples, the findings showed the negative correlations between the sperm nuclear protamine deficiency and progressive motility as well as immobility (p<0.001). CONCLUSION: The higher proportion of spermatozoa with abnormal chromatin packaging was observed in asthenospermic samples than those from other experimental groups as well as controls. It seems that normal morphology cannot have a valuable predictive value for good chromatin quality of spermatozoa, as much as normal motility characteristics, since samples with high mobility rates often have lower protamine deficiencies. The findings may provide a supportable promoting the future wider clinical application of chromatin/DNA integrity testing along with the semen analysis in male infertility. SN - 1698-0409 UR - https://www.unboundmedicine.com/medline/citation/31326281/Is_there_any_relationship_between_human_sperm_parameters_and_protamine_deficiency_in_different_groups_of_infertile_men L2 - https://linkinghub.elsevier.com/retrieve/pii/S1698-031X(19)30039-1 DB - PRIME DP - Unbound Medicine ER -
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