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Determining and unravelling origins of reduced photoinactivation efficacy of bacteria in milk.
J Photochem Photobiol B 2019; 197:111554JP

Abstract

Bovine mastitis is an endemic disease of dairy cattle that is considered to be one of the most frequent and costly diseases in veterinary medicine. An increase in the incidence of disease results in the increased use of antibiotics, which in turn increases the potential of bacterial resistance. This study aimed to investigate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of bovine mastitis, as an alternative to systemic antibiotics. To identify the key factors affecting photoinactivation efficacy, realistic experiments in view of the end-use were conducted in milk samples using two different photosensitizers: methylene blue (MB) and silicon (IV) phthalocyanine derivative (SiPc). We explored the effects of divalent ions and fat content on the aPDT outcome and determined influence of different proteins on aPDT efficacy. Levels of bacterial sensitivity to PSs varied depending on the type of bacteria (Gram-positive vs. Gram-negative) and light exposure time. Critical interrelated factors affecting aPDT in milk were identified and an efficient combination of treatment conditions that can lead to a full photodynamic inactivation of bacteria was determined.

Authors+Show Affiliations

Center for Soft Nanoscience, University of Münster, Busso-Peus-Straβe 10, D-48149 Münster, Germany. Electronic address: anzhela.galstyan@wwu.de.Institute of Hygiene, University of Münster, Mendelstraβe 7, D-48149 Münster, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31326843

Citation

Galstyan, Anzhela, and Ulrich Dobrindt. "Determining and Unravelling Origins of Reduced Photoinactivation Efficacy of Bacteria in Milk." Journal of Photochemistry and Photobiology. B, Biology, vol. 197, 2019, p. 111554.
Galstyan A, Dobrindt U. Determining and unravelling origins of reduced photoinactivation efficacy of bacteria in milk. J Photochem Photobiol B, Biol. 2019;197:111554.
Galstyan, A., & Dobrindt, U. (2019). Determining and unravelling origins of reduced photoinactivation efficacy of bacteria in milk. Journal of Photochemistry and Photobiology. B, Biology, 197, p. 111554. doi:10.1016/j.jphotobiol.2019.111554.
Galstyan A, Dobrindt U. Determining and Unravelling Origins of Reduced Photoinactivation Efficacy of Bacteria in Milk. J Photochem Photobiol B, Biol. 2019;197:111554. PubMed PMID: 31326843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determining and unravelling origins of reduced photoinactivation efficacy of bacteria in milk. AU - Galstyan,Anzhela, AU - Dobrindt,Ulrich, Y1 - 2019/07/12/ PY - 2019/05/15/received PY - 2019/06/30/revised PY - 2019/07/10/accepted PY - 2019/7/22/pubmed PY - 2019/8/3/medline PY - 2019/7/22/entrez SP - 111554 EP - 111554 JF - Journal of photochemistry and photobiology. B, Biology JO - J. Photochem. Photobiol. B, Biol. VL - 197 N2 - Bovine mastitis is an endemic disease of dairy cattle that is considered to be one of the most frequent and costly diseases in veterinary medicine. An increase in the incidence of disease results in the increased use of antibiotics, which in turn increases the potential of bacterial resistance. This study aimed to investigate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of bovine mastitis, as an alternative to systemic antibiotics. To identify the key factors affecting photoinactivation efficacy, realistic experiments in view of the end-use were conducted in milk samples using two different photosensitizers: methylene blue (MB) and silicon (IV) phthalocyanine derivative (SiPc). We explored the effects of divalent ions and fat content on the aPDT outcome and determined influence of different proteins on aPDT efficacy. Levels of bacterial sensitivity to PSs varied depending on the type of bacteria (Gram-positive vs. Gram-negative) and light exposure time. Critical interrelated factors affecting aPDT in milk were identified and an efficient combination of treatment conditions that can lead to a full photodynamic inactivation of bacteria was determined. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/31326843/Determining_and_unravelling_origins_of_reduced_photoinactivation_efficacy_of_bacteria_in_milk L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(19)30620-7 DB - PRIME DP - Unbound Medicine ER -