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First genotypic characterization of toxigenic Clostridioides difficile in Lithuanian hospitals reveals the prevalence of the hypervirulent ribotype 027/ST1.
Eur J Clin Microbiol Infect Dis. 2019 Oct; 38(10):1953-1959.EJ

Abstract

Clostridioides difficile has become the leading nosocomial Gram-positive pathogen in the developed countries. In Lithuania, the national surveillance program for C. difficile started in 2017. Enzyme immunoassay, the real-time PCR system, and culture are used for laboratory confirmation of C. difficile infection in Lithuanian clinical laboratories. No reference laboratory for C. difficile is present in Lithuania. Fifty-eight isolates of C. difficile were collected in 2016 and 2017 in two hospitals using real-time PCR and culture methods. Agarose gel-based PCR ribotyping, multilocus variable number tandem repeats analysis (MLVA), and multilocus sequence typing (MLST) were used for the genotypic characterization of 28 isolates. PCR ribotyping and MLST showed that 78.6% of the tested toxigenic isolates belong to the ribotype RT027/ST1. Using MLVA, 95.5% of RT027 isolates were genetically related. MLVA revealed three clonal complexes in RT027. Six non-RT027 isolates showed four different electrophoretic profiles in PCR ribotyping and were assigned to the MLST sequence types ST2, ST13, ST54, and ST63. The highest discriminatory power showed the genotyping by MLVA. In total, 20 MLVA profiles were identified. This genotyping technique allowed to identify four groups of RT027/ST1 isolates that were indistinguishable by PCR ribotyping and MLST. Our study is the first genotypic characterization of C. difficile isolates in Lithuania. We observed a high prevalence of presumptive RT027 that suggests unfavorable epidemiological situation in Lithuania. Our results stress for implementation of genotyping of C. difficile isolates in Lithuanian surveillance.

Authors+Show Affiliations

Vilnius City Clinical Hospital, Vilnius, Lithuania. Department of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania.Vilnius City Clinical Hospital, Vilnius, Lithuania.Department of Microbiology and Biotechnology, Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania. nomeda.kuisiene@gf.vu.lt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31327067

Citation

Tratulyte, Simona, et al. "First Genotypic Characterization of Toxigenic Clostridioides Difficile in Lithuanian Hospitals Reveals the Prevalence of the Hypervirulent Ribotype 027/ST1." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 38, no. 10, 2019, pp. 1953-1959.
Tratulyte S, Miciuleviciene J, Kuisiene N. First genotypic characterization of toxigenic Clostridioides difficile in Lithuanian hospitals reveals the prevalence of the hypervirulent ribotype 027/ST1. Eur J Clin Microbiol Infect Dis. 2019;38(10):1953-1959.
Tratulyte, S., Miciuleviciene, J., & Kuisiene, N. (2019). First genotypic characterization of toxigenic Clostridioides difficile in Lithuanian hospitals reveals the prevalence of the hypervirulent ribotype 027/ST1. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 38(10), 1953-1959. https://doi.org/10.1007/s10096-019-03633-9
Tratulyte S, Miciuleviciene J, Kuisiene N. First Genotypic Characterization of Toxigenic Clostridioides Difficile in Lithuanian Hospitals Reveals the Prevalence of the Hypervirulent Ribotype 027/ST1. Eur J Clin Microbiol Infect Dis. 2019;38(10):1953-1959. PubMed PMID: 31327067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First genotypic characterization of toxigenic Clostridioides difficile in Lithuanian hospitals reveals the prevalence of the hypervirulent ribotype 027/ST1. AU - Tratulyte,Simona, AU - Miciuleviciene,Jolanta, AU - Kuisiene,Nomeda, Y1 - 2019/07/20/ PY - 2019/05/18/received PY - 2019/07/08/accepted PY - 2019/7/22/pubmed PY - 2020/1/15/medline PY - 2019/7/22/entrez KW - Clostridioides difficile KW - Lithuania KW - MLST KW - MLVA KW - Ribotype 027 KW - Surveillance SP - 1953 EP - 1959 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 38 IS - 10 N2 - Clostridioides difficile has become the leading nosocomial Gram-positive pathogen in the developed countries. In Lithuania, the national surveillance program for C. difficile started in 2017. Enzyme immunoassay, the real-time PCR system, and culture are used for laboratory confirmation of C. difficile infection in Lithuanian clinical laboratories. No reference laboratory for C. difficile is present in Lithuania. Fifty-eight isolates of C. difficile were collected in 2016 and 2017 in two hospitals using real-time PCR and culture methods. Agarose gel-based PCR ribotyping, multilocus variable number tandem repeats analysis (MLVA), and multilocus sequence typing (MLST) were used for the genotypic characterization of 28 isolates. PCR ribotyping and MLST showed that 78.6% of the tested toxigenic isolates belong to the ribotype RT027/ST1. Using MLVA, 95.5% of RT027 isolates were genetically related. MLVA revealed three clonal complexes in RT027. Six non-RT027 isolates showed four different electrophoretic profiles in PCR ribotyping and were assigned to the MLST sequence types ST2, ST13, ST54, and ST63. The highest discriminatory power showed the genotyping by MLVA. In total, 20 MLVA profiles were identified. This genotyping technique allowed to identify four groups of RT027/ST1 isolates that were indistinguishable by PCR ribotyping and MLST. Our study is the first genotypic characterization of C. difficile isolates in Lithuania. We observed a high prevalence of presumptive RT027 that suggests unfavorable epidemiological situation in Lithuania. Our results stress for implementation of genotyping of C. difficile isolates in Lithuanian surveillance. SN - 1435-4373 UR - https://www.unboundmedicine.com/medline/citation/31327067/First_genotypic_characterization_of_toxigenic_Clostridioides_difficile_in_Lithuanian_hospitals_reveals_the_prevalence_of_the_hypervirulent_ribotype_027/ST1_ L2 - https://dx.doi.org/10.1007/s10096-019-03633-9 DB - PRIME DP - Unbound Medicine ER -