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[Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation].
Sheng Wu Gong Cheng Xue Bao 2019; 35(7):1266-1276SW

Abstract

In a one-step fermentation system of vitamin C production with Gluconobacter oxydans and Ketogulonicigenium vulgare, a functional module of α-lipoic acid biosynthesis was constructed in G. oxydans. The engineered G. oxydans was co-cultured with K. vulgare to enhance the growth and 2-keto-L-gulonic acid (2-KGA) production of K. vulgare. This one-step fermentation system alleviated the growth inhibition during the mono-culture of K. vulgare and strengthened the interaction between the two bacteria. Moreover, the yield of vitamin C precursor (2-KGA) increased to 73.34 g/L (the control group was 59.09 g/L), and the conversion of D-sorbitol to 2-KGA increased to 86.0%. This study provides a new idea for further optimizing the one-step fermentation system of vitamin C production.

Authors+Show Affiliations

Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31328483

Citation

Liu, Yu, et al. "[Biosynthesis of Α-lipoic Acid in Gluconobacter Oxydans Increases the Production of Vitamin C By One-step Fermentation]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 35, no. 7, 2019, pp. 1266-1276.
Liu Y, Wang E, Pan C, et al. [Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation]. Sheng Wu Gong Cheng Xue Bao. 2019;35(7):1266-1276.
Liu, Y., Wang, E., Pan, C., Dong, X., & Ding, M. (2019). [Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 35(7), pp. 1266-1276. doi:10.13345/j.cjb.190005.
Liu Y, et al. [Biosynthesis of Α-lipoic Acid in Gluconobacter Oxydans Increases the Production of Vitamin C By One-step Fermentation]. Sheng Wu Gong Cheng Xue Bao. 2019 Jul 25;35(7):1266-1276. PubMed PMID: 31328483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation]. AU - Liu,Yu, AU - Wang,Enxu, AU - Pan,Caihui, AU - Dong,Xiutao, AU - Ding,Mingzhu, PY - 2019/7/23/entrez PY - 2019/7/23/pubmed PY - 2019/8/30/medline KW - Gluconobacter oxydans KW - Ketogulonicigenium vulgare KW - one-step fermentation KW - α-lipoic acid SP - 1266 EP - 1276 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 35 IS - 7 N2 - In a one-step fermentation system of vitamin C production with Gluconobacter oxydans and Ketogulonicigenium vulgare, a functional module of α-lipoic acid biosynthesis was constructed in G. oxydans. The engineered G. oxydans was co-cultured with K. vulgare to enhance the growth and 2-keto-L-gulonic acid (2-KGA) production of K. vulgare. This one-step fermentation system alleviated the growth inhibition during the mono-culture of K. vulgare and strengthened the interaction between the two bacteria. Moreover, the yield of vitamin C precursor (2-KGA) increased to 73.34 g/L (the control group was 59.09 g/L), and the conversion of D-sorbitol to 2-KGA increased to 86.0%. This study provides a new idea for further optimizing the one-step fermentation system of vitamin C production. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/31328483/[Biosynthesis_of_α-lipoic_acid_in_Gluconobacter_oxydans_increases_the_production_of_vitamin_C_by_one-step_fermentation] L2 - https://doi.org/10.13345/j.cjb.190005 DB - PRIME DP - Unbound Medicine ER -