Tags

Type your tag names separated by a space and hit enter

tRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis.
Biomolecules 2019; 9(7)B

Abstract

tRNA-derived fragments (tRFs), a novel type of non-coding RNA derived from tRNAs, play an important part in governing gene expressions at a post-transcriptional level. To date, the regulatory mechanism of tRFs governing fat deposition and adipogenesis is completely unknown. In this study, high fat diet was employed to induce an obese rat model, and tRFs transcriptome sequencing was conducted to identify differentially expressed tRFs that response to obesity. We found out that tRFGluTTC, which promoted preadipocyte proliferation by increasing expressions of cell cycle regulatory factors, had the highest fold change in the 296 differentially expressed tRFs. Moreover, tRFGluTTC also suppressed preadipocyte differentiation by reducing triglyceride content and lipid accumulation, and by decreasing expressions of genes that related to fatty acid synthesis. According to results of luciferase activity analysis, tRFGluTTC directly targeted Kruppel-like factor (KLF) 9, KLF11, and KLF12, thus significantly suppressing mRNA expressions of these target genes. Moreover, tRFGluTTC suppressed adipogenesis, accompanying by suppressing expressions of adipogenic transcription factors (aP2, PPARγ, and C/EBPα). In conclusion, these results imply that tRFGluTTC may act as a novel epigenetic molecule regulating adipogenesis and could provide a new strategy for the intervention treatment of obesity.

Authors+Show Affiliations

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.Sichuan Province General Station of Animal Husbandry, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.Chongqing Academy of Animal Science, Chongqing 402460, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. zhangsh1919@163.com. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. zhangsh1919@163.com.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. zhuli7508@163.com. Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. zhuli7508@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31336727

Citation

Shen, Linyuan, et al. "TRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis." Biomolecules, vol. 9, no. 7, 2019.
Shen L, Tan Z, Gan M, et al. TRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis. Biomolecules. 2019;9(7).
Shen, L., Tan, Z., Gan, M., Li, Q., Chen, L., Niu, L., ... Zhu, L. (2019). TRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis. Biomolecules, 9(7), doi:10.3390/biom9070274.
Shen L, et al. TRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis. Biomolecules. 2019 07 11;9(7) PubMed PMID: 31336727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - tRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis. AU - Shen,Linyuan, AU - Tan,Zhendong, AU - Gan,Mailin, AU - Li,Qiang, AU - Chen,Lei, AU - Niu,Lili, AU - Jiang,Dongmei, AU - Zhao,Ye, AU - Wang,Jinyong, AU - Li,Xuewei, AU - Zhang,Shunhua, AU - Zhu,Li, Y1 - 2019/07/11/ PY - 2019/06/13/received PY - 2019/06/29/revised PY - 2019/07/08/accepted PY - 2019/7/25/entrez PY - 2019/7/25/pubmed PY - 2019/7/25/medline KW - 3T3-L1 KW - KLF family KW - adipogenesis KW - tRFs KW - transcriptome JF - Biomolecules JO - Biomolecules VL - 9 IS - 7 N2 - tRNA-derived fragments (tRFs), a novel type of non-coding RNA derived from tRNAs, play an important part in governing gene expressions at a post-transcriptional level. To date, the regulatory mechanism of tRFs governing fat deposition and adipogenesis is completely unknown. In this study, high fat diet was employed to induce an obese rat model, and tRFs transcriptome sequencing was conducted to identify differentially expressed tRFs that response to obesity. We found out that tRFGluTTC, which promoted preadipocyte proliferation by increasing expressions of cell cycle regulatory factors, had the highest fold change in the 296 differentially expressed tRFs. Moreover, tRFGluTTC also suppressed preadipocyte differentiation by reducing triglyceride content and lipid accumulation, and by decreasing expressions of genes that related to fatty acid synthesis. According to results of luciferase activity analysis, tRFGluTTC directly targeted Kruppel-like factor (KLF) 9, KLF11, and KLF12, thus significantly suppressing mRNA expressions of these target genes. Moreover, tRFGluTTC suppressed adipogenesis, accompanying by suppressing expressions of adipogenic transcription factors (aP2, PPARγ, and C/EBPα). In conclusion, these results imply that tRFGluTTC may act as a novel epigenetic molecule regulating adipogenesis and could provide a new strategy for the intervention treatment of obesity. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/31336727/tRNA_Derived_Small_Non_Coding_RNAs_as_Novel_Epigenetic_Molecules_Regulating_Adipogenesis_ L2 - http://www.mdpi.com/resolver?pii=biom9070274 DB - PRIME DP - Unbound Medicine ER -