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Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks.
Int J Mol Sci. 2019 Jul 23; 20(14)IJ

Abstract

Rootstocks frequently exert detrimental effects on the fruit quality of grafted cucumber (Cucumis sativus L.) plants. To understand and ultimately correct this deficiency, a transcriptomic and metabolomic comparative analysis was performed among cucumber fruits from non-grafted plants (NG), and fruits from plants grafted onto different rootstocks of No.96 and No.45 (Cucurbita moschata. Duch), known to confer a different aroma and taste. We found remarkable changes in the primary metabolites of sugars, organic acids, amino acids, and alcohols in the fruit of the grafted cucumber plants with different rootstocks, compared to the non-grafted ones, especially No.45. We identified 140, 131, and 244 differentially expressed genes (DEGs) in the comparisons of GNo.96 vs. NG, GNo.45 vs. NG, and GNo.45 vs. GNo.96. The identified DEGs have functions involved in many metabolic processes, such as starch and sucrose metabolism; the biosynthesis of diterpenoid, carotenoid, and zeatin compounds; and plant hormone signal transduction. Members of the HSF, AP2/ERF-ERF, HB-HD-ZIP, and MYB transcription factor families were triggered in the grafted cucumbers, especially in the cucumber grafted on No.96. Based on a correlation analysis of the relationships between the metabolites and genes, we screened 10 candidate genes likely to be involved in sugar metabolism (Fructose-6-phosphate and trehalose), linoleic acid, and amino-acid (isoleucine, proline, and valine) biosynthesis in grafted cucumbers, and then confirmed the gene expression patterns of these genes by qRT-PCR. The levels of TPS15 (Csa3G040850) were remarkably increased in cucumber fruit with No.96 rootstock compared with No.45, suggesting changes in the volatile chemical production. Together, the results of this study improve our understanding of flavor changes in grafted cucumbers, and identify the candidate genes involved in this process.

Authors+Show Affiliations

Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China. Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China. wangchanglin@caas.cn.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China. yuxianchang@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31340498

Citation

Miao, Li, et al. "Integrated Metabolome and Transcriptome Analysis Provide Insights Into the Effects of Grafting On Fruit Flavor of Cucumber With Different Rootstocks." International Journal of Molecular Sciences, vol. 20, no. 14, 2019.
Miao L, Di Q, Sun T, et al. Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks. Int J Mol Sci. 2019;20(14).
Miao, L., Di, Q., Sun, T., Li, Y., Duan, Y., Wang, J., Yan, Y., He, C., Wang, C., & Yu, X. (2019). Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks. International Journal of Molecular Sciences, 20(14). https://doi.org/10.3390/ijms20143592
Miao L, et al. Integrated Metabolome and Transcriptome Analysis Provide Insights Into the Effects of Grafting On Fruit Flavor of Cucumber With Different Rootstocks. Int J Mol Sci. 2019 Jul 23;20(14) PubMed PMID: 31340498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks. AU - Miao,Li, AU - Di,Qinghua, AU - Sun,Tianshu, AU - Li,Yansu, AU - Duan,Ying, AU - Wang,Jun, AU - Yan,Yan, AU - He,Chaoxing, AU - Wang,Changlin, AU - Yu,Xianchang, Y1 - 2019/07/23/ PY - 2019/06/16/received PY - 2019/07/08/revised PY - 2019/07/16/accepted PY - 2019/7/26/entrez PY - 2019/7/26/pubmed PY - 2019/12/28/medline KW - flavor KW - grafted cucumber KW - metabolomic KW - rootstock KW - sugars KW - transcriptomic JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 14 N2 - Rootstocks frequently exert detrimental effects on the fruit quality of grafted cucumber (Cucumis sativus L.) plants. To understand and ultimately correct this deficiency, a transcriptomic and metabolomic comparative analysis was performed among cucumber fruits from non-grafted plants (NG), and fruits from plants grafted onto different rootstocks of No.96 and No.45 (Cucurbita moschata. Duch), known to confer a different aroma and taste. We found remarkable changes in the primary metabolites of sugars, organic acids, amino acids, and alcohols in the fruit of the grafted cucumber plants with different rootstocks, compared to the non-grafted ones, especially No.45. We identified 140, 131, and 244 differentially expressed genes (DEGs) in the comparisons of GNo.96 vs. NG, GNo.45 vs. NG, and GNo.45 vs. GNo.96. The identified DEGs have functions involved in many metabolic processes, such as starch and sucrose metabolism; the biosynthesis of diterpenoid, carotenoid, and zeatin compounds; and plant hormone signal transduction. Members of the HSF, AP2/ERF-ERF, HB-HD-ZIP, and MYB transcription factor families were triggered in the grafted cucumbers, especially in the cucumber grafted on No.96. Based on a correlation analysis of the relationships between the metabolites and genes, we screened 10 candidate genes likely to be involved in sugar metabolism (Fructose-6-phosphate and trehalose), linoleic acid, and amino-acid (isoleucine, proline, and valine) biosynthesis in grafted cucumbers, and then confirmed the gene expression patterns of these genes by qRT-PCR. The levels of TPS15 (Csa3G040850) were remarkably increased in cucumber fruit with No.96 rootstock compared with No.45, suggesting changes in the volatile chemical production. Together, the results of this study improve our understanding of flavor changes in grafted cucumbers, and identify the candidate genes involved in this process. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31340498/Integrated_Metabolome_and_Transcriptome_Analysis_Provide_Insights_into_the_Effects_of_Grafting_on_Fruit_Flavor_of_Cucumber_with_Different_Rootstocks_ L2 - https://www.mdpi.com/resolver?pii=ijms20143592 DB - PRIME DP - Unbound Medicine ER -