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Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii.
Cells 2019; 8(7)C

Abstract

Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.

Authors+Show Affiliations

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo 04023-062, Brazil.Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo 04023-062, Brazil.Laboratório Especial de Ciclo Celular-Center of Toxins, Immune Response and Cell Signaling-Center (CeTICS), Butantan Institute, São Paulo 05503-900, Brazil.Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA.Instituto Carlos Chagas-FIOCRUZ PR, Curitiba 81350-010, Brazil. Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.Instituto Carlos Chagas-FIOCRUZ PR, Curitiba 81350-010, Brazil.Instituto Carlos Chagas-FIOCRUZ PR, Curitiba 81350-010, Brazil.Instituto Carlos Chagas-FIOCRUZ PR, Curitiba 81350-010, Brazil. lys.alves@gmail.com.Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo 04023-062, Brazil. ropuccia@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31340551

Citation

Peres da Silva, Roberta, et al. "Comparison of the RNA Content of Extracellular Vesicles Derived From Paracoccidioides Brasiliensis and Paracoccidioides Lutzii." Cells, vol. 8, no. 7, 2019.
Peres da Silva R, Longo LGV, Cunha JPCD, et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells. 2019;8(7).
Peres da Silva, R., Longo, L. G. V., Cunha, J. P. C. D., Sobreira, T. J. P., Rodrigues, M. L., Faoro, H., ... Puccia, R. (2019). Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells, 8(7), doi:10.3390/cells8070765.
Peres da Silva R, et al. Comparison of the RNA Content of Extracellular Vesicles Derived From Paracoccidioides Brasiliensis and Paracoccidioides Lutzii. Cells. 2019 07 23;8(7) PubMed PMID: 31340551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. AU - Peres da Silva,Roberta, AU - Longo,Larissa G V, AU - Cunha,Julia P C da, AU - Sobreira,Tiago J P, AU - Rodrigues,Marcio L, AU - Faoro,Helisson, AU - Goldenberg,Samuel, AU - Alves,Lysangela R, AU - Puccia,Rosana, Y1 - 2019/07/23/ PY - 2019/05/30/received PY - 2019/07/09/revised PY - 2019/07/13/accepted PY - 2019/7/26/entrez PY - 2019/7/26/pubmed PY - 2019/7/26/medline KW - Paracoccidiodes KW - RNA-seq KW - extracellular vesicles KW - mRNA KW - sRNA JF - Cells JO - Cells VL - 8 IS - 7 N2 - Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/31340551/Comparison_of_the_RNA_Content_of_Extracellular_Vesicles_Derived_from_Paracoccidioides_brasiliensis_and_Paracoccidioides_lutzii L2 - http://www.mdpi.com/resolver?pii=cells8070765 DB - PRIME DP - Unbound Medicine ER -