Tags

Type your tag names separated by a space and hit enter

Circular RNA circ_0008450 upregulates CXCL9 expression by targeting miR-577 to regulate cell proliferation and invasion in nasopharyngeal carcinoma.
Exp Mol Pathol 2019; 110:104288EM

Abstract

As a kind of malignant tumor, nasopharyngeal carcinoma (NPC) has attracted increasing attention from researchers. As a member of the circular RNA (circRNA) family, circ_0008450 has been investigated in hepatocellular carcinoma but not in NPC. This study aims to reveal the special biologic role and mechanism of circ_0008450 in NPC. qRT-PCR analysis was conducted to test the level of circ_0008450 in different tissues and cells. Loss/Gain of function assay was utilized to detect the influence of silenced/overexpressed circ_0008450 on the proliferation, apoptosis, migration, and invasion of NPC cells. The mechanism of circ_0008450 was assessed by performing qRT-PCR and luciferase reporter experiments. The results showed that circ_0008450 was elevated in NPC tissues and cells. Silenced circ_0008450 could inhibit cell proliferation, and metastatic properties and increased the number of apoptotic cells. Ectopically expressed circ_0008450 strengthened the abovementioned malignant biological behaviors. Mechanistically, circ_0008450 reduced miR-577-mediated repression of CXCL9, resulting in facilitating the oncogenic functions of NPC. In conclusion, circ_0008450 acts as an oncogene in NPC cells through regulating miR-577/CXCL9 signaling. Our findings might provide a new therapeutic target for treating NPC.

Authors+Show Affiliations

Department of Gynecology and Pediatrics Nursing, Qiqihar Medical University, Qiqihar 161000, China.Department of Biobank, Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161000, China.Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161000, China.Department of Pharmacy, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.Department of Medical Service, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161000, China.Department of Microform Experiment Center, College of Pathology, Qiqihar Medical University, Qiqihar 161000, China.Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161000, China. Electronic address: galaxyun@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31344361

Citation

Wei, Hongjuan, et al. "Circular RNA Circ_0008450 Upregulates CXCL9 Expression By Targeting miR-577 to Regulate Cell Proliferation and Invasion in Nasopharyngeal Carcinoma." Experimental and Molecular Pathology, vol. 110, 2019, p. 104288.
Wei H, Liu D, Sun J, et al. Circular RNA circ_0008450 upregulates CXCL9 expression by targeting miR-577 to regulate cell proliferation and invasion in nasopharyngeal carcinoma. Exp Mol Pathol. 2019;110:104288.
Wei, H., Liu, D., Sun, J., Mao, Y., Zhao, L., Zhu, W., ... Gao, Z. (2019). Circular RNA circ_0008450 upregulates CXCL9 expression by targeting miR-577 to regulate cell proliferation and invasion in nasopharyngeal carcinoma. Experimental and Molecular Pathology, 110, p. 104288. doi:10.1016/j.yexmp.2019.104288.
Wei H, et al. Circular RNA Circ_0008450 Upregulates CXCL9 Expression By Targeting miR-577 to Regulate Cell Proliferation and Invasion in Nasopharyngeal Carcinoma. Exp Mol Pathol. 2019;110:104288. PubMed PMID: 31344361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circular RNA circ_0008450 upregulates CXCL9 expression by targeting miR-577 to regulate cell proliferation and invasion in nasopharyngeal carcinoma. AU - Wei,Hongjuan, AU - Liu,Deshui, AU - Sun,Jia, AU - Mao,Ying, AU - Zhao,Lihui, AU - Zhu,Wenbin, AU - Xu,Guangyou, AU - Gao,Zhiying, Y1 - 2019/07/22/ PY - 2019/04/11/received PY - 2019/07/14/revised PY - 2019/07/19/accepted PY - 2019/7/26/pubmed PY - 2019/7/26/medline PY - 2019/7/26/entrez KW - CXCL9 KW - Circ_0008450 KW - Circular RNA KW - Nasopharyngeal carcinoma KW - miR-577 SP - 104288 EP - 104288 JF - Experimental and molecular pathology JO - Exp. Mol. Pathol. VL - 110 N2 - As a kind of malignant tumor, nasopharyngeal carcinoma (NPC) has attracted increasing attention from researchers. As a member of the circular RNA (circRNA) family, circ_0008450 has been investigated in hepatocellular carcinoma but not in NPC. This study aims to reveal the special biologic role and mechanism of circ_0008450 in NPC. qRT-PCR analysis was conducted to test the level of circ_0008450 in different tissues and cells. Loss/Gain of function assay was utilized to detect the influence of silenced/overexpressed circ_0008450 on the proliferation, apoptosis, migration, and invasion of NPC cells. The mechanism of circ_0008450 was assessed by performing qRT-PCR and luciferase reporter experiments. The results showed that circ_0008450 was elevated in NPC tissues and cells. Silenced circ_0008450 could inhibit cell proliferation, and metastatic properties and increased the number of apoptotic cells. Ectopically expressed circ_0008450 strengthened the abovementioned malignant biological behaviors. Mechanistically, circ_0008450 reduced miR-577-mediated repression of CXCL9, resulting in facilitating the oncogenic functions of NPC. In conclusion, circ_0008450 acts as an oncogene in NPC cells through regulating miR-577/CXCL9 signaling. Our findings might provide a new therapeutic target for treating NPC. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/31344361/Circular_RNA_circ_0008450_upregulates_CXCL9_expression_by_targeting_miR-577_to_regulate_cell_proliferation_and_invasion_in_nasopharyngeal_carcinoma L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(19)30263-1 DB - PRIME DP - Unbound Medicine ER -