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Proteomics of Naja kaouthia venom from North East India and assessment of Indian polyvalent antivenom by third generation antivenomics.
J Proteomics. 2019 09 15; 207:103463.JP

Abstract

In the present study, venom composition, toxic effects, and immunological characteristics of Naja kaouthia venom from North East India has been studied. Using RP-HPLC, venom components were separated and proteins in the fractions were identified using ESI-LC MS/MS. Proteins identified belong to 9 different snake venom protein families. Three finger toxins and PLA2 were the most abundant protein families detected by mass spectrometry analysis. The other minor proteins families identified in the venom were kunitz-type serine inhibitors, waprin, L-amino acid oxidase, CRISP, vespyrn, nerve growth factor and metalloproteinase. This proteome composition correlated with the tested enzymatic and toxic activities of the venom. Western blot and third generation antivenomics analysis using Vins polyvalent antivenom revealed immunoreactivity towards Naja kaouthia venom of North East India. Concentration-dependent immunocapturing profile carried out using RP-HPLC displayed immunerecognition of majority of venom proteins of Naja kaouthia except few three-finger toxins. Presence of such non-immunodepleted toxins apparently may affect the performance of Vins polyvalent antivenom. Thus, inclusion of antibodies of most relevant non-immunorecognized toxins in antivenom might help to improve the quality of antivenom. BIOLOGICAL

SIGNIFICANCE:

Envenomings by genus Naja, represent a serious medical problem in Asian countries including North east India. In North East India, Naja kaouthia is most prevalent cobra species causing a large number of fatalities. To gain deeper insight into the spectrum of medically relevant toxins, we applied proteomics approach to unveil the proteome profile of Naja kaouthia venom. The proteomic analysis divulged the presence of two major protein families: three finger toxins and phospholipases A2. In general, polyvalent antivenom is administered for Naja kaouthia envenomings, however, this venom is not included in the immunization mixtures (only Indian Big Four venoms) for production of these polyvalent antivenoms. For the first time, third generation antivenomics approach was used to decipher maximal binding capacity of Indian polyvalent antivenom against Naja kaouthia venom. Although Vins polyvalent antivenom was effective in immunocapturing majority of venom components, however, large amount of antivenom was required to immunocapture the venom proteins. Moreover, the study revealed poor immunorecognition capacity of Vins antivenom towards four three finger toxin subtypes. This may have significant impact on antivenom efficacy in treating Naja kaouthia envenomings.

Authors+Show Affiliations

Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India.Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India.Department of Neurological Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.Help Earth, RNC Path, Lachitnagar, Guwahati, Assam 781007, India.Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India. Electronic address: doley@tezu.ernet.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31344496

Citation

Deka, Archana, et al. "Proteomics of Naja Kaouthia Venom From North East India and Assessment of Indian Polyvalent Antivenom By Third Generation Antivenomics." Journal of Proteomics, vol. 207, 2019, p. 103463.
Deka A, Gogoi A, Das D, et al. Proteomics of Naja kaouthia venom from North East India and assessment of Indian polyvalent antivenom by third generation antivenomics. J Proteomics. 2019;207:103463.
Deka, A., Gogoi, A., Das, D., Purkayastha, J., & Doley, R. (2019). Proteomics of Naja kaouthia venom from North East India and assessment of Indian polyvalent antivenom by third generation antivenomics. Journal of Proteomics, 207, 103463. https://doi.org/10.1016/j.jprot.2019.103463
Deka A, et al. Proteomics of Naja Kaouthia Venom From North East India and Assessment of Indian Polyvalent Antivenom By Third Generation Antivenomics. J Proteomics. 2019 09 15;207:103463. PubMed PMID: 31344496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomics of Naja kaouthia venom from North East India and assessment of Indian polyvalent antivenom by third generation antivenomics. AU - Deka,Archana, AU - Gogoi,Aditi, AU - Das,Diganta, AU - Purkayastha,Jayaditya, AU - Doley,Robin, Y1 - 2019/07/22/ PY - 2019/05/24/received PY - 2019/07/15/revised PY - 2019/07/21/accepted PY - 2019/7/26/pubmed PY - 2020/9/4/medline PY - 2019/7/26/entrez KW - Antivenom KW - Antivenomics KW - Naja kaouthia KW - Snake venom KW - Third-generation antivenomics KW - Venomics SP - 103463 EP - 103463 JF - Journal of proteomics JO - J Proteomics VL - 207 N2 - In the present study, venom composition, toxic effects, and immunological characteristics of Naja kaouthia venom from North East India has been studied. Using RP-HPLC, venom components were separated and proteins in the fractions were identified using ESI-LC MS/MS. Proteins identified belong to 9 different snake venom protein families. Three finger toxins and PLA2 were the most abundant protein families detected by mass spectrometry analysis. The other minor proteins families identified in the venom were kunitz-type serine inhibitors, waprin, L-amino acid oxidase, CRISP, vespyrn, nerve growth factor and metalloproteinase. This proteome composition correlated with the tested enzymatic and toxic activities of the venom. Western blot and third generation antivenomics analysis using Vins polyvalent antivenom revealed immunoreactivity towards Naja kaouthia venom of North East India. Concentration-dependent immunocapturing profile carried out using RP-HPLC displayed immunerecognition of majority of venom proteins of Naja kaouthia except few three-finger toxins. Presence of such non-immunodepleted toxins apparently may affect the performance of Vins polyvalent antivenom. Thus, inclusion of antibodies of most relevant non-immunorecognized toxins in antivenom might help to improve the quality of antivenom. BIOLOGICAL SIGNIFICANCE: Envenomings by genus Naja, represent a serious medical problem in Asian countries including North east India. In North East India, Naja kaouthia is most prevalent cobra species causing a large number of fatalities. To gain deeper insight into the spectrum of medically relevant toxins, we applied proteomics approach to unveil the proteome profile of Naja kaouthia venom. The proteomic analysis divulged the presence of two major protein families: three finger toxins and phospholipases A2. In general, polyvalent antivenom is administered for Naja kaouthia envenomings, however, this venom is not included in the immunization mixtures (only Indian Big Four venoms) for production of these polyvalent antivenoms. For the first time, third generation antivenomics approach was used to decipher maximal binding capacity of Indian polyvalent antivenom against Naja kaouthia venom. Although Vins polyvalent antivenom was effective in immunocapturing majority of venom components, however, large amount of antivenom was required to immunocapture the venom proteins. Moreover, the study revealed poor immunorecognition capacity of Vins antivenom towards four three finger toxin subtypes. This may have significant impact on antivenom efficacy in treating Naja kaouthia envenomings. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/31344496/Proteomics_of_Naja_kaouthia_venom_from_North_East_India_and_assessment_of_Indian_polyvalent_antivenom_by_third_generation_antivenomics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(19)30235-0 DB - PRIME DP - Unbound Medicine ER -