Tags

Type your tag names separated by a space and hit enter

MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism.
J Agric Food Chem 2019; 67(32):8986-8993JA

Abstract

Trehalose plays a crucial role in response to freezing stress in baker's yeast. MAL62, a gene involved in the adenosine diphosphoglucose-dependent trehalose synthesis pathway, can increase trehalose content. However, the difference between MAL62-related trehalose synthesis and traditional uridine diphosphoglucose-dependent trehalose synthesis is not well-understood. MAL62 overexpression showed less effect in enhancing intracellular trehalose compared to TPS1 overexpression. However, MAL62 overexpression elicited trehalose synthesis before fermentation with enhanced maltose metabolism and had a similar effect on cell viability after freezing. Furthermore, MAL62 and TPS1 overexpression in the NTH1 deletion background further strengthened freezing tolerance and improved leavening ability. Our results suggest that the enhancement in freezing tolerance by MAL62 overexpression may involve multiple pathways rather than simply enhancing trehalose synthesis. The results reveal valuable insights into the relationship between maltose metabolism and freezing tolerance and may help to develop better yeast strains for enhancing fermentation characteristics of frozen dough.

Authors+Show Affiliations

Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.Tianjin Engineering Research Center of Agricultural Products Processing , Tianjin 300384 , People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31347835

Citation

Sun, Xi, et al. "MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough By Enhancing Tps1 Activity and Maltose Metabolism." Journal of Agricultural and Food Chemistry, vol. 67, no. 32, 2019, pp. 8986-8993.
Sun X, Zhang J, Fan ZH, et al. MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism. J Agric Food Chem. 2019;67(32):8986-8993.
Sun, X., Zhang, J., Fan, Z. H., Xiao, P., Liu, S. N., Li, R. P., ... Huang, L. (2019). MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism. Journal of Agricultural and Food Chemistry, 67(32), pp. 8986-8993. doi:10.1021/acs.jafc.9b03790.
Sun X, et al. MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough By Enhancing Tps1 Activity and Maltose Metabolism. J Agric Food Chem. 2019 Aug 14;67(32):8986-8993. PubMed PMID: 31347835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism. AU - Sun,Xi, AU - Zhang,Jun, AU - Fan,Zhi-Hua, AU - Xiao,Ping, AU - Liu,Shan-Na, AU - Li,Rui-Peng, AU - Zhu,Wen-Bi, AU - Huang,Liang, Y1 - 2019/08/06/ PY - 2019/7/28/pubmed PY - 2019/8/27/medline PY - 2019/7/27/entrez KW - KW - baker’s yeast KW - cell viability KW - freezing tolerance KW - leavening ability SP - 8986 EP - 8993 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 67 IS - 32 N2 - Trehalose plays a crucial role in response to freezing stress in baker's yeast. MAL62, a gene involved in the adenosine diphosphoglucose-dependent trehalose synthesis pathway, can increase trehalose content. However, the difference between MAL62-related trehalose synthesis and traditional uridine diphosphoglucose-dependent trehalose synthesis is not well-understood. MAL62 overexpression showed less effect in enhancing intracellular trehalose compared to TPS1 overexpression. However, MAL62 overexpression elicited trehalose synthesis before fermentation with enhanced maltose metabolism and had a similar effect on cell viability after freezing. Furthermore, MAL62 and TPS1 overexpression in the NTH1 deletion background further strengthened freezing tolerance and improved leavening ability. Our results suggest that the enhancement in freezing tolerance by MAL62 overexpression may involve multiple pathways rather than simply enhancing trehalose synthesis. The results reveal valuable insights into the relationship between maltose metabolism and freezing tolerance and may help to develop better yeast strains for enhancing fermentation characteristics of frozen dough. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/31347835/MAL62_Overexpression_Enhances_Freezing_Tolerance_of_Baker's_Yeast_in_Lean_Dough_by_Enhancing_Tps1_Activity_and_Maltose_Metabolism L2 - https://dx.doi.org/10.1021/acs.jafc.9b03790 DB - PRIME DP - Unbound Medicine ER -