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A miniaturized device for biomembrane permeation analysis.

Abstract

Transdermal drug delivery is widely investigated as an alternative drug administration route to oral delivery and hypodermic injections. Owing to the availability of human skin samples, in vitro tests are used to predict the in vivo delivery of transdermal drugs. The most widely used validation method is skin permeation using diffusion cells. Traditional diffusion cells, however, are capacious and often require large amounts of skin sample and drugs, which is undesirable, given the scarcity of new drug entities and the limitation of skin sample supply. In this study, we fabricated miniaturized multichannel devices (MCDs) by 3D printing, to minimize the use of skin and drug samples. The MCDs were compared with conventional static diffusion cells and achieved comparable drug permeation profiles. The finite element method-based simulation revealed the efficient carry-off of permeated ingredients by the multichannel devices, and a critical role of distance between the buffer stream and skin sample in determining the flow velocity inside the chamber. The results support these devices as qualified alternatives to Franz cells for in vitro permeation studies using biomembranes, with reduced use of skin and drug samples.

Authors+Show Affiliations

College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai Road, Suzhou 215123, China.Skinetrate Pte Ltd, 79 Ayer Rajah Crescent, Singapore 139955, Singapore.Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore.Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore.Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore.Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China. Electronic address: cywu@cpu.edu.cn.Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore.School of Pharmacy, University of Sydney, Pharmacy and Bank Building A15, NSW 2006, Australia. Electronic address: lifeng.kang@sydney.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31349518

Citation

Ding, Dawei, et al. "A Miniaturized Device for Biomembrane Permeation Analysis." Materials Science & Engineering. C, Materials for Biological Applications, vol. 103, 2019, p. 109772.
Ding D, Pan J, Yeo SH, et al. A miniaturized device for biomembrane permeation analysis. Mater Sci Eng C Mater Biol Appl. 2019;103:109772.
Ding, D., Pan, J., Yeo, S. H., Wagholikar, V., Lim, S. H., Wu, C., ... Kang, L. (2019). A miniaturized device for biomembrane permeation analysis. Materials Science & Engineering. C, Materials for Biological Applications, 103, p. 109772. doi:10.1016/j.msec.2019.109772.
Ding D, et al. A Miniaturized Device for Biomembrane Permeation Analysis. Mater Sci Eng C Mater Biol Appl. 2019;103:109772. PubMed PMID: 31349518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A miniaturized device for biomembrane permeation analysis. AU - Ding,Dawei, AU - Pan,Jing, AU - Yeo,Shih Hui, AU - Wagholikar,Vishal, AU - Lim,Seng Han, AU - Wu,Chunyong, AU - Fuh,Jerry Y H, AU - Kang,Lifeng, Y1 - 2019/05/18/ PY - 2018/12/14/received PY - 2019/04/18/revised PY - 2019/05/17/accepted PY - 2019/7/28/entrez KW - 3D printing KW - Diffusion cell KW - Finite element method KW - Membrane KW - Transdermal drug delivery SP - 109772 EP - 109772 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 103 N2 - Transdermal drug delivery is widely investigated as an alternative drug administration route to oral delivery and hypodermic injections. Owing to the availability of human skin samples, in vitro tests are used to predict the in vivo delivery of transdermal drugs. The most widely used validation method is skin permeation using diffusion cells. Traditional diffusion cells, however, are capacious and often require large amounts of skin sample and drugs, which is undesirable, given the scarcity of new drug entities and the limitation of skin sample supply. In this study, we fabricated miniaturized multichannel devices (MCDs) by 3D printing, to minimize the use of skin and drug samples. The MCDs were compared with conventional static diffusion cells and achieved comparable drug permeation profiles. The finite element method-based simulation revealed the efficient carry-off of permeated ingredients by the multichannel devices, and a critical role of distance between the buffer stream and skin sample in determining the flow velocity inside the chamber. The results support these devices as qualified alternatives to Franz cells for in vitro permeation studies using biomembranes, with reduced use of skin and drug samples. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/31349518/A_miniaturized_device_for_biomembrane_permeation_analysis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(18)33679-8 DB - PRIME DP - Unbound Medicine ER -