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Transforming compound leaf patterning by manipulating REVOLUTA in Medicago truncatula.
Plant J 2019PJ

Abstract

Leaves are derived from the shoot apical meristem with three distinct axes: dorsoventral, proximodistal and mediolateral. Different regulators are involved in the establishment of leaf polarity. Members of the class III homeodomain-leucine zipper (HD-ZIPIII) gene family are critical players in the determination of leaf adaxial identity mediated by microRNA165/166. However, their roles in compound leaf development are still unclear. By screening of a retrotransposon-tagged mutant population of the model legume plant Medicago truncatula, a mutant line with altered leaflet numbers was isolated and characterized. Mutant leaves partially lost their adaxial identity. Leaflet numbers in the mutant were increased along the proximodistal axis, showing pinnate pentafoliate leaves in most cases, in contrast to the trifoliate leaves of the wild type. Detailed characterization revealed that a lesion in a HD-ZIPIII gene, REVOLUTA (MtREV1), resulted in the defects of the mutant. Overexpression of MtMIR166-insensitive MtREV1 led to adaxialized leaves and ectopic leaflets along the dorsoventral axis. Accompanying the abnormal leaf patterning, the free auxin content was affected. Our results demonstrate that MtREV1 plays a key role in determination of leaf adaxial-abaxial polarity and compound leaf patterning, which is associated with proper auxin homeostasis.

Authors+Show Affiliations

The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.Noble Research Institute, LLC, Ardmore, OK, 73401, USA.Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, Hunan, 410128, China.Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, Hunan, 410128, China.Noble Research Institute, LLC, Ardmore, OK, 73401, USA. Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31350797

Citation

Zhou, Chuanen, et al. "Transforming Compound Leaf Patterning By Manipulating REVOLUTA in Medicago Truncatula." The Plant Journal : for Cell and Molecular Biology, 2019.
Zhou C, Han L, Zhao Y, et al. Transforming compound leaf patterning by manipulating REVOLUTA in Medicago truncatula. Plant J. 2019.
Zhou, C., Han, L., Zhao, Y., Wang, H., Nakashima, J., Tong, J., ... Wang, Z. Y. (2019). Transforming compound leaf patterning by manipulating REVOLUTA in Medicago truncatula. The Plant Journal : for Cell and Molecular Biology, doi:10.1111/tpj.14469.
Zhou C, et al. Transforming Compound Leaf Patterning By Manipulating REVOLUTA in Medicago Truncatula. Plant J. 2019 Jul 26; PubMed PMID: 31350797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transforming compound leaf patterning by manipulating REVOLUTA in Medicago truncatula. AU - Zhou,Chuanen, AU - Han,Lu, AU - Zhao,Yang, AU - Wang,Hongfeng, AU - Nakashima,Jin, AU - Tong,Jianhua, AU - Xiao,Langtao, AU - Wang,Zeng-Yu, Y1 - 2019/07/26/ PY - 2019/02/25/received PY - 2019/06/27/accepted PY - 2019/7/28/pubmed PY - 2019/7/28/medline PY - 2019/7/28/entrez KW - Medicago KW - REVOLUTA KW - HD-ZIPIII gene family KW - compound leaf patterning KW - leaf polarity JF - The Plant journal : for cell and molecular biology JO - Plant J. N2 - Leaves are derived from the shoot apical meristem with three distinct axes: dorsoventral, proximodistal and mediolateral. Different regulators are involved in the establishment of leaf polarity. Members of the class III homeodomain-leucine zipper (HD-ZIPIII) gene family are critical players in the determination of leaf adaxial identity mediated by microRNA165/166. However, their roles in compound leaf development are still unclear. By screening of a retrotransposon-tagged mutant population of the model legume plant Medicago truncatula, a mutant line with altered leaflet numbers was isolated and characterized. Mutant leaves partially lost their adaxial identity. Leaflet numbers in the mutant were increased along the proximodistal axis, showing pinnate pentafoliate leaves in most cases, in contrast to the trifoliate leaves of the wild type. Detailed characterization revealed that a lesion in a HD-ZIPIII gene, REVOLUTA (MtREV1), resulted in the defects of the mutant. Overexpression of MtMIR166-insensitive MtREV1 led to adaxialized leaves and ectopic leaflets along the dorsoventral axis. Accompanying the abnormal leaf patterning, the free auxin content was affected. Our results demonstrate that MtREV1 plays a key role in determination of leaf adaxial-abaxial polarity and compound leaf patterning, which is associated with proper auxin homeostasis. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/31350797/Transforming_compound_leaf_patterning_by_manipulating_REVOLUTA_in_Medicago_truncatula L2 - https://doi.org/10.1111/tpj.14469 DB - PRIME DP - Unbound Medicine ER -