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Activated T cells and monocytes have characteristic patterns of class II antigen expression.
J Immunol. 1988 Aug 15; 141(4):1281-7.JI

Abstract

The expression of human histocompatibility class II Ag was measured on activated T cells and monocytes by quantitative mAb binding in direct two-color immunofluorescence. Monocytes activated by IFN-gamma bound an average of 2 x 10(6) DR-specific mAb, 3 x 10(5) DQ-specific mAb, and 7 x 10(5) DP-specific mAb per cell. For T cells activated by anti-CD3, a subpopulation bound 1 x 10(5) DR-specific mAb, 5 x 10(4) DQ-specific mAb and 5 x 10(4) DP-specific mAb per cell. These measurements were obtained after establishing a base line of class II Ag expression on resting B cells and monocytes. Resting B cells and those monocytes that were positive for class II Ag bound identical amounts of mAb; 3 x 10(4) DR-specific mAb, 3 x 10(3) DQ-specific mAb and 2 x 10(4) DP-specific mAb. However, most resting monocytes (75%) expressed only DR Ag. In the process of studying the expression of class II Ag on T cells, it was necessary to define and analyze the activated T cell state. Cell cultures activated with 0.3 ng/ml anti-CD3 had the highest expression of class II Ag on T cells, whereas those activated with 3.0 ng/ml anti-CD3 had the highest expression of IL-2R on T cells. Addition of IL-2 had no further effect on DR Ag expression on T cells but did up-regulate IL-2R expression. Reducing the initial monocyte concentration before activating T cells increased class II Ag expression on T cells without affecting IL-2R expression. The results obtained on T cell activation suggest that perhaps a lymphokine may be made by CD3-activated T cells which induces class II Ag expression on T cells.

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Authors+Show Affiliations

Robbins PA
Becton Dickinson Immunocytometry Systems, Mountain View, CA 94043.
Maino VC
No affiliation info available
Warner NL
No affiliation info available
Brodsky FM
No affiliation info available

MeSH

Antibodies, MonoclonalFluorescein-5-isothiocyanateFluoresceinsHLA-D AntigensHLA-DP AntigensHLA-DQ AntigensHLA-DR AntigensHumansInterphaseLymphocyte ActivationMacrophage ActivationMonocytesPhenotypeT-LymphocytesThiocyanates

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3135327

Citation

Robbins, P A., et al. "Activated T Cells and Monocytes Have Characteristic Patterns of Class II Antigen Expression." Journal of Immunology (Baltimore, Md. : 1950), vol. 141, no. 4, 1988, pp. 1281-7.
Robbins PA, Maino VC, Warner NL, et al. Activated T cells and monocytes have characteristic patterns of class II antigen expression. J Immunol. 1988;141(4):1281-7.
Robbins, P. A., Maino, V. C., Warner, N. L., & Brodsky, F. M. (1988). Activated T cells and monocytes have characteristic patterns of class II antigen expression. Journal of Immunology (Baltimore, Md. : 1950), 141(4), 1281-7.
Robbins PA, et al. Activated T Cells and Monocytes Have Characteristic Patterns of Class II Antigen Expression. J Immunol. 1988 Aug 15;141(4):1281-7. PubMed PMID: 3135327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activated T cells and monocytes have characteristic patterns of class II antigen expression. AU - Robbins,P A, AU - Maino,V C, AU - Warner,N L, AU - Brodsky,F M, PY - 1988/8/15/pubmed PY - 1988/8/15/medline PY - 1988/8/15/entrez SP - 1281 EP - 7 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 141 IS - 4 N2 - The expression of human histocompatibility class II Ag was measured on activated T cells and monocytes by quantitative mAb binding in direct two-color immunofluorescence. Monocytes activated by IFN-gamma bound an average of 2 x 10(6) DR-specific mAb, 3 x 10(5) DQ-specific mAb, and 7 x 10(5) DP-specific mAb per cell. For T cells activated by anti-CD3, a subpopulation bound 1 x 10(5) DR-specific mAb, 5 x 10(4) DQ-specific mAb and 5 x 10(4) DP-specific mAb per cell. These measurements were obtained after establishing a base line of class II Ag expression on resting B cells and monocytes. Resting B cells and those monocytes that were positive for class II Ag bound identical amounts of mAb; 3 x 10(4) DR-specific mAb, 3 x 10(3) DQ-specific mAb and 2 x 10(4) DP-specific mAb. However, most resting monocytes (75%) expressed only DR Ag. In the process of studying the expression of class II Ag on T cells, it was necessary to define and analyze the activated T cell state. Cell cultures activated with 0.3 ng/ml anti-CD3 had the highest expression of class II Ag on T cells, whereas those activated with 3.0 ng/ml anti-CD3 had the highest expression of IL-2R on T cells. Addition of IL-2 had no further effect on DR Ag expression on T cells but did up-regulate IL-2R expression. Reducing the initial monocyte concentration before activating T cells increased class II Ag expression on T cells without affecting IL-2R expression. The results obtained on T cell activation suggest that perhaps a lymphokine may be made by CD3-activated T cells which induces class II Ag expression on T cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3135327/Activated_T_cells_and_monocytes_have_characteristic_patterns_of_class_II_antigen_expression_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3135327 DB - PRIME DP - Unbound Medicine ER -