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High sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolates.
PLoS One. 2019; 14(7):e0220671.Plos

Abstract

Clostridium [Clostridioides] difficile infection (CDI) is one of the leading causes of diarrhea associated with medical care worldwide, and up to 60% of patients with CDI can develop a recurrent infection (R-CDI). A multi-species microbiota biofilm model of C. difficile was designed to evaluate the differences in the production of biofilms, sporulation, susceptibility to drugs, expression of sporulating (sigH, spo0A), quorum sensing (agrD1, and luxS), and adhesion-associated (slpA and cwp84) pathway genes between selected C. difficile isolates from R-CDI and non-recurrent patients (NR-CDI). We obtained 102 C. difficile isolates from 254 patients with confirmed CDI (66 from NR-CDI and 36 from R-CDI). Most of the isolates were biofilm producers, and most of the strains were ribotype 027 (81.374%, 83/102). Most C. difficile isolates were producers of biofilm (100/102), and most were strongly adherent. Sporulation was higher in the R-CDI than in the NR-CDI isolates (p = 0.015). The isolates from R-CDI patients more frequently demonstrated reduced susceptibility to vancomycin than isolates of NR-CDI patients (27.78% [10/36] and 9.09% [6/66], respectively, p = 0.013). The minimum inhibitory concentrations for vancomycin and linezolid against biofilms (BMIC) were up to 100 times and 20 times higher, respectively, than the corresponding planktonic MICs. Expression of sigH, spo0A, cwp84, and agrD1 was higher in R-CDI than in NR-CDI isolates. Most of the C. difficile isolates were producers of biofilms with no correlation with the ribotype. Sporulation was greater in R-CDI than in NR-CDI isolates in the biofilm model of C. difficile. The R-CDI isolates more frequently demonstrated reduced susceptibility to vancomycin and linezolid than the NR-CDI isolates in both planktonic cells and biofilm isolates. A higher expression of sporulating pathway (sigH, spo0A), quorum sensing (agrD1), and adhesion-associated (cwp84) genes was found in R-CDI than in NR-CDI isolates. All of these factors can have effect on the recurrence of the infection.

Authors+Show Affiliations

Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.Department of Biological and Environmental Sciences, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom.Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital Civil de Guadalajara "Fray Antonio Alcalde", Guadalajara, Mexico.Hospital Universitario "Dr. José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Mexico.Hospital Universitario "Dr. José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Mexico.Hospital Universitario "Dr. José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Mexico.Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Hospital Civil de Guadalajara "Fray Antonio Alcalde", Guadalajara, Mexico.Hospital Universitario "Dr. José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Mexico.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31365590

Citation

Tijerina-Rodríguez, Laura, et al. "High Sporulation and Overexpression of Virulence Factors in Biofilms and Reduced Susceptibility to Vancomycin and Linezolid in Recurrent Clostridium [Clostridioides] Difficile Infection Isolates." PloS One, vol. 14, no. 7, 2019, pp. e0220671.
Tijerina-Rodríguez L, Villarreal-Treviño L, Baines SD, et al. High sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolates. PLoS ONE. 2019;14(7):e0220671.
Tijerina-Rodríguez, L., Villarreal-Treviño, L., Baines, S. D., Morfín-Otero, R., Camacho-Ortíz, A., Flores-Treviño, S., Maldonado-Garza, H., Rodríguez-Noriega, E., & Garza-González, E. (2019). High sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolates. PloS One, 14(7), e0220671. https://doi.org/10.1371/journal.pone.0220671
Tijerina-Rodríguez L, et al. High Sporulation and Overexpression of Virulence Factors in Biofilms and Reduced Susceptibility to Vancomycin and Linezolid in Recurrent Clostridium [Clostridioides] Difficile Infection Isolates. PLoS ONE. 2019;14(7):e0220671. PubMed PMID: 31365590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolates. AU - Tijerina-Rodríguez,Laura, AU - Villarreal-Treviño,Licet, AU - Baines,Simon D, AU - Morfín-Otero,Rayo, AU - Camacho-Ortíz,Adrián, AU - Flores-Treviño,Samantha, AU - Maldonado-Garza,Héctor, AU - Rodríguez-Noriega,Eduardo, AU - Garza-González,Elvira, Y1 - 2019/07/31/ PY - 2019/05/30/received PY - 2019/07/20/accepted PY - 2019/8/1/entrez PY - 2019/8/1/pubmed PY - 2020/3/5/medline SP - e0220671 EP - e0220671 JF - PloS one JO - PLoS ONE VL - 14 IS - 7 N2 - Clostridium [Clostridioides] difficile infection (CDI) is one of the leading causes of diarrhea associated with medical care worldwide, and up to 60% of patients with CDI can develop a recurrent infection (R-CDI). A multi-species microbiota biofilm model of C. difficile was designed to evaluate the differences in the production of biofilms, sporulation, susceptibility to drugs, expression of sporulating (sigH, spo0A), quorum sensing (agrD1, and luxS), and adhesion-associated (slpA and cwp84) pathway genes between selected C. difficile isolates from R-CDI and non-recurrent patients (NR-CDI). We obtained 102 C. difficile isolates from 254 patients with confirmed CDI (66 from NR-CDI and 36 from R-CDI). Most of the isolates were biofilm producers, and most of the strains were ribotype 027 (81.374%, 83/102). Most C. difficile isolates were producers of biofilm (100/102), and most were strongly adherent. Sporulation was higher in the R-CDI than in the NR-CDI isolates (p = 0.015). The isolates from R-CDI patients more frequently demonstrated reduced susceptibility to vancomycin than isolates of NR-CDI patients (27.78% [10/36] and 9.09% [6/66], respectively, p = 0.013). The minimum inhibitory concentrations for vancomycin and linezolid against biofilms (BMIC) were up to 100 times and 20 times higher, respectively, than the corresponding planktonic MICs. Expression of sigH, spo0A, cwp84, and agrD1 was higher in R-CDI than in NR-CDI isolates. Most of the C. difficile isolates were producers of biofilms with no correlation with the ribotype. Sporulation was greater in R-CDI than in NR-CDI isolates in the biofilm model of C. difficile. The R-CDI isolates more frequently demonstrated reduced susceptibility to vancomycin and linezolid than the NR-CDI isolates in both planktonic cells and biofilm isolates. A higher expression of sporulating pathway (sigH, spo0A), quorum sensing (agrD1), and adhesion-associated (cwp84) genes was found in R-CDI than in NR-CDI isolates. All of these factors can have effect on the recurrence of the infection. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31365590/High_sporulation_and_overexpression_of_virulence_factors_in_biofilms_and_reduced_susceptibility_to_vancomycin_and_linezolid_in_recurrent_Clostridium_[Clostridioides]_difficile_infection_isolates_ L2 - http://dx.plos.org/10.1371/journal.pone.0220671 DB - PRIME DP - Unbound Medicine ER -