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[Acupuncture up-regulates MCT2 expression of peri-ischemic cortex in middle cerebral artery occlusion rats].
Zhen Ci Yan Jiu 2019; 44(6):399-404ZC

Abstract

OBJECTIVE

To observe the effect of manual acupuncture (MA)+ electroacupuncture (EA) on changes of neurological function and expression of monocarboxylate transporter 2(MCT2)in cerebral ischemia (CI) rats, so as to explore its mechanism underlying improvement of ischemic cerebrovascular disease.

METHODS

Eighty male Wistar rats were equally randomized into four groups: normal control (normal), sham operation (sham), model and acupuncture. The CI model was induced by middle cerebral artery occlusion (MCAO) with a thread embolus. Manual acupuncture stimulation (mild twisting reinforcing-reducing method) was applied to "Baihui"(GV20)and "Fengfu"(GV16) for 10 min. EA (1 mA, 2 Hz /15 Hz) was respectively applied to bilateral "Quchi" (LI11) and "Zusanli"(ST36) for 20 min, once per day for 7 days. The neurological deficit severity was evaluated according to Zea Longa's methods. The activity of lactate dehydrogenase (LDH), fructose-6-phosphate kinase (PFK) and pyruvate kinase (PK) in the peri-ischemic cortex tissue was detected by enzymatic chemistry, and the expression of MCT2 detected by immunofluorescence histochemistry, Western blot and quantitative real-time PCR, separately.

RESULTS

After CI and in comparison with the normal and sham groups, the Zea Longa's score, the fluorescence intensity and the expression level of MCT2 protein were significantly increased (P<0.01), and the activity of LDH, PFK and PK in the peri-ischemic cortex was significantly decreased in the model group (P<0.01). There was no significant change in the relative expression of MCT2 mRNA (P>0.05). Following the intervention and in comparison with the model group, the Zea Longa's score was considerably decreased in the acupuncture group (P<0.01), the activity of LDH, PFK and PK,and the expression levels of MCT2 protein and mRNA were considerably or further up-regulated in the acupuncture group (P<0.01, P<0.05).

CONCLUSION

Acupuncture intervention can improve neurological function in CI rats, which is possibly related with its effects in up-regulating the expression of MCT2 and promoting the utilization of lactate in peri-ischemic cortex.

Authors+Show Affiliations

College of Acupuncture-moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.The First Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011.College of Chinese Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355.College of Chinese Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355.College of Chinese Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355.College of Chinese Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31368261

Citation

Lu, Yan, et al. "[Acupuncture Up-regulates MCT2 Expression of Peri-ischemic Cortex in Middle Cerebral Artery Occlusion Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 6, 2019, pp. 399-404.
Lu Y, Yin Y, Zhao HJ, et al. [Acupuncture up-regulates MCT2 expression of peri-ischemic cortex in middle cerebral artery occlusion rats]. Zhen Ci Yan Jiu. 2019;44(6):399-404.
Lu, Y., Yin, Y., Zhao, H. J., Wang, Y., Han, B. B., & Wang, S. J. (2019). [Acupuncture up-regulates MCT2 expression of peri-ischemic cortex in middle cerebral artery occlusion rats]. Zhen Ci Yan Jiu = Acupuncture Research, 44(6), pp. 399-404. doi:10.13702/j.1000-0607.180836.
Lu Y, et al. [Acupuncture Up-regulates MCT2 Expression of Peri-ischemic Cortex in Middle Cerebral Artery Occlusion Rats]. Zhen Ci Yan Jiu. 2019 Jun 25;44(6):399-404. PubMed PMID: 31368261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Acupuncture up-regulates MCT2 expression of peri-ischemic cortex in middle cerebral artery occlusion rats]. AU - Lu,Yan, AU - Yin,Ying, AU - Zhao,Hai-Jun, AU - Wang,Yuan, AU - Han,Bing-Bing, AU - Wang,Shi-Jun, PY - 2019/8/2/entrez PY - 2019/8/2/pubmed PY - 2019/8/8/medline KW - Acupuncture KW - Focal cerebral ischemia KW - Monocarboxylate transporter 2 KW - Neurological function SP - 399 EP - 404 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 6 N2 - OBJECTIVE: To observe the effect of manual acupuncture (MA)+ electroacupuncture (EA) on changes of neurological function and expression of monocarboxylate transporter 2(MCT2)in cerebral ischemia (CI) rats, so as to explore its mechanism underlying improvement of ischemic cerebrovascular disease. METHODS: Eighty male Wistar rats were equally randomized into four groups: normal control (normal), sham operation (sham), model and acupuncture. The CI model was induced by middle cerebral artery occlusion (MCAO) with a thread embolus. Manual acupuncture stimulation (mild twisting reinforcing-reducing method) was applied to "Baihui"(GV20)and "Fengfu"(GV16) for 10 min. EA (1 mA, 2 Hz /15 Hz) was respectively applied to bilateral "Quchi" (LI11) and "Zusanli"(ST36) for 20 min, once per day for 7 days. The neurological deficit severity was evaluated according to Zea Longa's methods. The activity of lactate dehydrogenase (LDH), fructose-6-phosphate kinase (PFK) and pyruvate kinase (PK) in the peri-ischemic cortex tissue was detected by enzymatic chemistry, and the expression of MCT2 detected by immunofluorescence histochemistry, Western blot and quantitative real-time PCR, separately. RESULTS: After CI and in comparison with the normal and sham groups, the Zea Longa's score, the fluorescence intensity and the expression level of MCT2 protein were significantly increased (P<0.01), and the activity of LDH, PFK and PK in the peri-ischemic cortex was significantly decreased in the model group (P<0.01). There was no significant change in the relative expression of MCT2 mRNA (P>0.05). Following the intervention and in comparison with the model group, the Zea Longa's score was considerably decreased in the acupuncture group (P<0.01), the activity of LDH, PFK and PK,and the expression levels of MCT2 protein and mRNA were considerably or further up-regulated in the acupuncture group (P<0.01, P<0.05). CONCLUSION: Acupuncture intervention can improve neurological function in CI rats, which is possibly related with its effects in up-regulating the expression of MCT2 and promoting the utilization of lactate in peri-ischemic cortex. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31368261/[Acupuncture_up_regulates_MCT2_expression_of_peri_ischemic_cortex_in_middle_cerebral_artery_occlusion_rats]_ L2 - https://medlineplus.gov/acupuncture.html DB - PRIME DP - Unbound Medicine ER -